A5027819245- Microbial Natural Products and Biosynthesis
- Genomics and Phylogenetic Studies
- RNA and protein synthesis mechanisms
- Actinomycetales infections and treatment
- Protein Kinase Regulation and GTPase Signaling
- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- Fungal Biology and Applications
- Cellular Mechanics and Interactions
- Antimicrobial Peptides and Activities
- Plant biochemistry and biosynthesis
- Cancer Treatment and Pharmacology
- Biochemical and Structural Characterization
- Cell Adhesion Molecules Research
- Microbial Metabolic Engineering and Bioproduction
- Radiopharmaceutical Chemistry and Applications
- Synthetic Organic Chemistry Methods
- Marine Sponges and Natural Products
- Physiological and biochemical adaptations
- Chemical Synthesis and Analysis
- PI3K/AKT/mTOR signaling in cancer
- DNA Repair Mechanisms
- Hearing, Cochlea, Tinnitus, Genetics
- Cellular transport and secretion
- Neurobiology and Insect Physiology Research
Mylan (Switzerland)
2019
Biogen (Italy)
2015
IFOM
2006-2013
University of Gothenburg
2003
European Institute of Oncology
2001-2002
Ripamonti
2002
University of Milan
1998-2001
Signaling from receptor tyrosine kinases (RTKs)* requires the sequential activation of small GTPases Ras and Rac. Son sevenless (Sos-1), a bifunctional guanine nucleotide exchange factor (GEF), activates in vivo displays Rac-GEF activity vitro, when engaged tricomplex with Eps8 E3b1–Abi-1, RTK substrate an adaptor protein, respectively. A mechanistic understanding how Sos-1 coordinates Rac is, however, still missing. Here, we demonstrate that (a) Sos-1, E3b1, assemble into under...
Mechanotransduction in the mammalian auditory system depends on mechanosensitive channels hair bundles that project from apical surface of sensory cells. Individual stereocilia within each bundle contain a core tightly packed actin filaments, whose length is dynamically regulated during development and adult. We show actin-binding protein epidermal growth factor receptor pathway substrate 8 (Eps8)L2, member Eps8-like family, newly identified localized at tips both cochlear vestibular It has...
Abstract The glycopeptide A40926, produced by the actinomycete Nonomuraea gerenzanensis , is precursor of dalbavancin, a second-generation antibiotic approved for clinical use in USA and Europe 2014 2015, respectively. final product biosynthetic pathway an O- acetylated form A40926 (acA40926). Glycopeptide biosynthesis N. dependent upon dbv gene cluster that encodes, addition to two essential positive regulators Dbv3 Dbv4, putative members two-component signal transduction system,...
Genetic and biochemical evidence demonstrated that Eps8 is involved in the routing of signals from Ras to Rac. This achieved through formation a tricomplex consisting Eps8-E3b1-Sos-1, which endowed with Rac guanine nucleotide exchange activity. The catalytic subunit this complex represented by Sos-1, bifunctional molecule capable catalyzing on mechanism Sos-1 activity specifically directed toward remains be established. Here, performing structure-function analysis we show output function...
Background In a variety of organisms, including mammals, caloric restriction improves metabolic status and lowers the incidence chronic-degenerative diseases, ultimately leading to increased lifespan. Methodology/Principal Findings Here we show that knockout mice for Eps8, regulator actin dynamics, display reduced body weight, partial resistance age- or diet-induced obesity, overall improved status. Alteration in liver gene expression profile, behavior metabolism point calorie...
GE2270 is a thiopeptide antibiotic generated by extensive posttranslational modifications of ribosomally precursor peptide. Thiopeptides are especially active against Gram-positive bacteria, including methicillin resistant Staphylococcus aureus (MRSA). In this study the biosynthetic gene cluster (pbt) from Planobispora rosea ATCC 53733 was successfully expressed in heterologous host strain Streptomyces coelicolor M1146. Notably, exconjugants containing pbt could only be obtained after...
We report the genome sequence of Planobispora rosea ATCC 53733, a mycelium-forming soil-dweller belonging to one lesser studied genera Actinobacteria and producing thiopeptide GE2270. The P. presents considerable convergence in gene organization function with other members family Streptosporangiaceae, significant number (44%) shared orthologs. Patterns expression cultures during exponential stationary phase have been analyzed using whole transcriptome shotgun sequencing by proteome analysis....
In screening for novel antibiotics, an attractive element of novelty can be represented by previously underexplored groups microorganisms. We report the results 200 strains belonging to actinobacterial genus Actinoallomurus their production antibacterial compounds. When grown under just one condition, about half produced extract that was able inhibit growth Staphylococcus aureus. here on metabolites 37 strains. addition reported aminocoumarins, lantibiotics and aromatic polyketides, we...
NAI-112, a glycosylated, labionine-containing lanthipeptide with weak antibacterial activity, has demonstrated analgesic activity in relevant mouse models of nociceptive and neuropathic pain. However, the mechanism(s) through which NAI-112 exerts its activities is not known. In this study, we analyzed changes spinal cord lipidome resulting from treatment naive in-pain mice. Notably, led to an increase phosphatidic acid levels both no-pain pain decrease lysophosphatidic model only. We also...
In the search for structurally novel metabolites with antibacterial activity, innovative approaches must be implemented to increase probability of discovering chemistry from microbial sources. Here we report on application metabolomic tools genus Actinoallomurus, a poorly explored member Actinobacteria. From examining extracts derived 88 isolates belonging this genus, identified family cyclodepsipeptides acylated C20 polyketide chain, which named allopeptimicins. These molecules possess...
In the Escherichia coli phage-plasmid P4, two partially overlapping replicons with bipartite ori sites coexist. The essential components of oriI replicon are alpha and cnr genes ori1 crr sites; oriII is composed gene, internal ori2 site, region. P4 protein has primase helicase activities specifically binds type I iterons, present in crr. Using a complementation test for plasmid replication, we demonstrated that depend on both protein. Moreover, neither requires host DnaA, DnaG, Rep...
ABSTRACT Natural products are a sustainable resource for drug discovery, but their identification in complex mixtures remains daunting task. We present an automated pipeline that compares, harmonizes and ranks the annotations of LC-HRMS data by different tools. When applied to 7,400 extracts derived from 6,566 strains belonging 86 actinomycete genera, it yielded 150,000 molecules after processing over 50 million MS features. The web-based Molecules Gateway provides highly interactive access...
Natural products are a sustainable resource for drug discovery, but their identification in complex mixtures remains daunting task. We present an automated pipeline that compares, harmonizes and ranks the annotations of LC-HRMS data by different tools. When applied to 7,400 extracts derived from 6,566 strains belonging 86 actinomycete genera, it yielded 150,000 molecules after processing over 50 million MS features. The web-based Molecules Gateway provides highly interactive access...
DNA replication of phage-plasmid P4 in its host Escherichia coli depends on protein α. In the plasmid state, copy number is controlled by regulator Cnr (copy regulation). Mutations α (αcr) that prevent regulation cause over-replication and cell death. Using two-hybrid system Saccharomyces cerevisiae a based λ immunity E.coli for vivo detection protein–protein interactions, we found that: (i) interacts with Cnr, whereas αcr proteins do not; (ii) both α–α αcr–αcr interactions occur interaction...
Abstract In the search for structurally novel metabolites with antibacterial activity, innovative approaches must be implemented to increase probability of discovering chemistry from microbial sources. Here we report on application metabolomic tools genus Actinoallomurus , a poorly explored member Actinobacteria. From examining extracts derived 88 isolates belonging this genus, identified family cyclodepsipeptides acylated C 20 polyketide chain, which named allopeptimicins. These molecules...