A5029587764- Organ Transplantation Techniques and Outcomes
- Liver Disease and Transplantation
- Cancer, Hypoxia, and Metabolism
- Photodynamic Therapy Research Studies
- Liver Disease Diagnosis and Treatment
- Nanoplatforms for cancer theranostics
- Porphyrin and Phthalocyanine Chemistry
- Heat shock proteins research
- Liver physiology and pathology
- Cancer Cells and Metastasis
- thermodynamics and calorimetric analyses
- Cardiac Ischemia and Reperfusion
- Erythrocyte Function and Pathophysiology
- Angiogenesis and VEGF in Cancer
- Porphyrin Metabolism and Disorders
- Immune cells in cancer
- Ultrasound and Hyperthermia Applications
- Lipid metabolism and biosynthesis
- Gastroesophageal reflux and treatments
- Organ Donation and Transplantation
- Autophagy in Disease and Therapy
- Coffee research and impacts
- Neuropeptides and Animal Physiology
- Mitochondrial Function and Pathology
- Sulfur Compounds in Biology
Universidade Estadual Paulista (Unesp)
2025
Universidade Federal de Minas Gerais
2024
Universidade do Estado de Minas Gerais
2024
Hospital Universitário Walter Cantídio
2021
University of Pavia
2007-2017
Istituto di Genetica Molecolare
2008-2014
Istituto Nazionale per le Malattie Infettive Lazzaro Spallanzani
2013
Hospital Dr. Nélio Mendonça
2012-2013
Universidade da Madeira
2013
Fresenius Medical Care (United States)
2012
We tested whether rat liver preservation performed by machine perfusion (MP) at 20 degrees C can enhance the functional integrity of steatotic livers versus simple cold storage. also compared MP with hypothermic 8 C, and 4 C. Obese lean male Zucker rats were used as donors. was for 6 hours a glucose N-acetylcysteine-supplemented Krebs-Henseleit solution. Both storage preserved reperfused solution (2 37 C). reduced fatty necrosis but we further protected organs using Necrosis did not differ...
Ischemia/reperfusion injury (IRI) associated with liver transplantation plays an important role in the induction of graft injury. Prolonged cold storage remains a risk factor for outcome, especially when steatosis is present. Steatotic livers exhibit exacerbated endoplasmic reticulum (ER) stress that occurs response to IRI. In addition, defective autophagy correlates well damage. Here, we evaluated combined effect melatonin and trimetazidine as additives IGL-1 solution modulation ER...
Abstract: Chronic organ‐donor shortage has required the acceptance of steatotic livers for transplantation purposes despite higher risk graft dysfunction or nonfunction associated with cold ischemia–reperfusion injury. This study evaluated use melatonin as an additive to Institute Georges Lopez (IGL‐1) solution protecting nonsteatotic and liver grafts against In current investigation, we used ex vivo isolated perfused rat model. Steatotic were preserved 24 hr (4°C) in University Wisconsin...
AIM:To examine the relevance of hypoxia inducible factor (HIF-1) and nitric oxide (NO) on preservation fatty liver against cold ischemia-reperfusion injury (IRI). METHODS:We used an isolated perfused rat model we evaluated HIF-1α in steatotic non-steatotic livers preserved for 24 h at 4℃ University Wisconsin IGL-1 solutions, then subjected to 2 normothermic reperfusion.After normoxic reperfusion, enzymes, bile production, bromosulfophthalein clearance, as well NO [endothelial synthase (eNOS)...
After surgical tooth extraction, it is necessary to maintain the environment of oral cavity in appropriate conditions favor alveolar bone repair. Coffee includes multiple bioactive compounds; some these compounds can affect metabolism. The aim this study was investigate effect coffee ingestion on dynamics after extraction using confocal laser microscopy. Eight male Wistar rats, aged two months, were used and divided into groups: control group, which normal diet maintained, experimental with...
A digital lock-in detection technique which overcomes some practical limitations of the analog amplifiers is described. An example implementation given, based on multichannel pulse analyzers. Some representative results are reported for two typical application fields techniques, namely optical spectrometry and semiconductor profiling with C–V techniques. Advantages limitation discussed as well adaptation to various modulation waveforms.
Abstract Background and Objective Autofluorescence (AF) emission is an intrinsic parameter that can provide real‐time information on morpho‐functional properties of biological tissue, being strictly related with their biochemical composition structural organization. The diagnostic potentials AF‐based techniques have been investigated normal, fibrotic, steatotic liver tissues, in reference to histological features as evidenced by specific histochemical stainings. Materials Methods AF under...
Abstract: Although the use of melatonin in transplantation field has been suggested, it not previously tested a liver cold‐storage model. We used rat model to study (a) dose‐dependent effect on bile production, and (b) potential improve function after cold‐storage. Male Wistar rats were perfused with Krebs–Henseleit bicarbonate buffer (KHB) at 37°C without or 25, 50, 100 200 μ m melatonin. Each dose stimulated production. For studies, livers flushed either University Wisconsin (UW) Celsior...
Nonalcoholic fatty liver disease (NAFLD) is a serious health problem in developed countries. We documented the effects of feeding with NAFLD-inducing, methionine- and choline-deficient (MCD) diet, for 1-4 weeks on rat oxidative stress, respect to control diet. Glycogen, neutral lipids, ROS, peroxidated proteins, SOD2 were investigated using histochemical procedures; ATP, GSH, TBARS concentrations by biochemical dosages, expression was Western Blotting. In 4-week-diet period, glycogen stores...
This study compares the effects of machine perfusion (MP) at different temperatures with simple cold storage. In addition, role Ca2+ levels in MP medium was evaluated. For MP, rat livers were perfused for 6 hours Krebs-Henseleit (KH) solution (with 1.25 or 2.5 mM CaCl2) 4°C, 10°C, 20°C, 25°C, 30°C, 37°C. storage, situ and preserved Celsior 4°C hours. The reperfusion period (2 37°C) performed under same conditions used MP-preserved storage–preserved livers. Hepatic enzyme release, bile...
Machine perfusion at subnormothermic temperature (20°C), MP20, was developed by Vairetti et al. and showed to afford a better preservation of fatty livers respect traditional cold storage (CS) in terms enzyme release into the perfusate bile, glycogen stores, energy charge oxidative stress. Here we investigated whether it also caused decreased cell death apoptosis. Fatty lean Zucker rats were submitted MP20 or CS for 6 h reperfused normothermically 2 h. Apoptotic cells revealed...
Studies assessing the effects of partial-hepatic ischemia/reperfusion (I/R) injury focused on damage to ischemic-lobe, whereas few data are available non-ischemic lobe. This study investigated whether acute liver I/R does affect lobe function via modulation extracellular matrix remodeling. Male Sprague-Dawley rats underwent left lateral- and median-lobe ischemia for 30 min reperfusion 60 or sham operation. After reperfusion, blood samples hepatic biopsies from both ischemic (left-lobe, LL)...