A5074636544- Streptococcal Infections and Treatments
- Antimicrobial Resistance in Staphylococcus
- Bacterial biofilms and quorum sensing
- Neonatal and Maternal Infections
- Biochemical and Structural Characterization
- Dental materials and restorations
- Endodontics and Root Canal Treatments
- Pneumonia and Respiratory Infections
- Antimicrobial Peptides and Activities
- Force Microscopy Techniques and Applications
- Oral microbiology and periodontitis research
- Dental Anxiety and Anesthesia Techniques
- Cell Adhesion Molecules Research
- Dental Radiography and Imaging
- Dental Erosion and Treatment
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Bacterial Identification and Susceptibility Testing
- Infective Endocarditis Diagnosis and Management
- Toxin Mechanisms and Immunotoxins
- Orthopedic Infections and Treatments
- Immune Response and Inflammation
- Monoclonal and Polyclonal Antibodies Research
- Polymer composites and self-healing
- Blood properties and coagulation
- Blood disorders and treatments
University of Pavia
2016-2025
Laboratoire de Biochimie
2020
Policlinico San Matteo Fondazione
2018
Politecnico di Milano
2006
Universität Ulm
2004
Trinity College Dublin
2004
Istituto Nazionale per le Malattie Infettive Lazzaro Spallanzani
2002
Background: This study compared the solubility and pH of different root canal sealers in vitro.Material Methods: BioRoot™RCS, TotalFill BC Sealer, MTA Fillapex, SealapexTM, AH Plus, EasySeal, Pulp Canal Sealer™ N2 were tested.Similar specimens prepared using ring molds with an internal diameter 20 ± 0,1 mm a height 1,5 digitally weighted to register mass each specimen before after immersion distilled water.Solubility was determined 24 hours statistically analysed one-way ANOVA test post-hoc...
The purpose of the present study was to compare biological and physico-chemical properties bioceramic-based root canal sealers, calcium hydroxide-based, MTA-based epoxy resin-based sealers.Two one hydroxide-based sealer, sealer two sealers were tested.EasySeal MTA Fillapex showed severe citotoxic activity, AH Plus SealapexTM moderate cytotoxicity, BioRoot™ RCS TotalFill BC Sealer both cytocompatible. Except for Sealer, all caused inhibition zones when tested with E. faecalis. highest zone...
ABSTRACT The bacterial pathogen Staphylococcus aureus expresses a variety of cell surface adhesion proteins that bind to host extracellular matrix proteins. Among these, the collagen (Cn)-binding protein Cna plays important roles in bacterium-host adherence and immune evasion. While it is well established A region mediates ligand binding, whether repetitive B has dedicated function not known. Here, we report direct measurement mechanical strength Cna-Cn bonds on living bacteria, quantify...
Staphylococcus aureus can invade various types of mammalian cells, thereby enabling it to evade host immune defenses and antibiotics. The current model for cellular invasion involves the interaction between bacterial cell surface located fibronectin (Fn)-binding proteins (FnBPA FnBPB) α5β1 integrin in membrane. While is believed that extracellular matrix protein Fn serves as a bridging molecule FnBPs integrins, fundamental forces involved are not known. Using single-cell single-molecule...
Streptococcus agalactiae is a major cause of bacterial pneumonia, sepsis, and meningitis in human neonates. During the course infection, S. adheres to variety epithelial cells but underlying mechanisms are only poorly understood. The present report demonstrates importance fibrinogen receptor FbsA for streptococcal adherence invasion cells. Deletion fbsA gene various strains substantially reduced their binding soluble cells, indicating role these different processes. invasiveness an deletion...
Background: The aim of the present study was to compare in vitro antimicrobial activity different root canal sealers against Enterococcus faecalis, prior and subsequent setting.Material Methods: Agar diffusion test (ADT) used for evaluating antibacterial non-set sealer while direct contact (DCT) after setting.Results: ADT: Except TotalFill BC Sealer all others tested showed activity.BioRoot™RCS, MTA Fillapex Sealapex Root Canal lowest activity, a significant increase effect both Pulp Sealer™...
Staphylococcus aureus is a commensal bacterium that has the ability to cause superficial and deep-seated infections. Like several other invasive pathogens, S. can capture plasminogen from human host where it be converted plasmin by activators or endogenously expressed staphylokinase. This study demonstrates sortase-anchored cell wall-associated proteins are responsible for capturing bulk of bound plasminogen. Two proteins, fibrinogen- fibronectin-binding A B, were found bind plasminogen, one...
The collagen-binding protein Cna is a prototype cell surface from Staphylococcus aureus which fulfils important physiological functions during pathogenesis. While it established that binds to collagen (Cn) via the high-affinity hug mechanism, whether this engaged in other ligand-binding mechanisms poorly understood. Here, we use atomic force microscopy demonstrate mediates attachment two structurally and functionally different host proteins, i.e., complement system C1q extracellular matrix...
Staphylococcus aureus is a Gram-positive bacterium that can cause both superficial and deep-seated infections. Histones released by neutrophils kill bacteria binding to the bacterial cell surface causing membrane damage. We postulated wall-anchored proteins protect S. from bactericidal effects of histones sequestering away envelope. Here, we focused on strain LAC using an array biochemical assays, including plasmon resonance ELISA, discovered fibronectin-binding protein B (FnBPB) main...
Abstract Physical forces have profound effects on cellular behavior, physiology, and disease. Perhaps the most intruiguing fascinating example is formation of catch-bonds that strengthen adhesion under shear stresses. Today mannose-binding by Escherichia coli FimH adhesin remains one rare microbial catch-bond thoroughly characterized at molecular level. Here we provide a quantitative demonstration in living Gram-positive pathogens using force-clamp spectroscopy. We show dock, lock, latch...
The aim of the present study is to evaluate and compare cytotoxic effects eight root canal sealers (BioRoot RCS, TotalFill BC Sealer, MTA Fillapex, Sealapex, AH Plus, EasySeal, Pulp Canal N2) on immortalized human gingival fibroblasts over a period 24, 48 72 hours.Immortalized fibroblast-1 HGF-1 (ATCC CRL-2014) were incubated. Root then placed into sterile, cylindrical Teflon moulds. extraction was made eluting in cell culture medium. Cells (1 × 104) seeded each well 96-well plate incubated...
ABSTRACT Staphylococcus lugdunensis is a coagulase-negative staphylococcus that commensal of humans and an opportunistic pathogen. It can cause spectrum infections, including those are associated with the ability to form biofilm, such as occurs endocarditis or indwelling medical devices. The genome sequences two strains revealed presence orthologues ica genes responsible for synthesis poly- N -acetylglucosamine (PNAG) commonly biofilm in other staphylococci. However, we discovered formed by...
In the present study, antimicrobial properties of a new ozonized olive oil (O-zone gel) against oral and periodontal pathogens will be evaluated compared with that common CHX-based agents.O-zone gel was two agents based on chlorhexidine digluconate (CHX): Corsodyl Dental Gel Plak Gel. A. actinomycetemcomitans, P. intermedia S. mutans, were selected antibacterial capability compounds tested by using direct contact agar diffusion test (DCT) minimum inhibitory concentration (MIC) bactericidal...
ABSTRACT Invasive bacterial pathogens can capture host plasminogen (Plg) and allow it to form plasmin. This process is of medical importance as surface-bound plasmin promotes spread by cleaving tissue components favors immune evasion degrading opsonins. In Staphylococcus aureus , Plg binding in part mediated cell surface fibronectin-binding proteins (FnBPs), but the underlying molecular mechanism not known. Here, we use single-cell single-molecule techniques demonstrate that FnBPs a...