A5048761325- Bacterial biofilms and quorum sensing
- Lipid Membrane Structure and Behavior
- Force Microscopy Techniques and Applications
- Alzheimer's disease research and treatments
- Biochemical and Structural Characterization
- Antimicrobial Resistance in Staphylococcus
- Origins and Evolution of Life
- Cellular Mechanics and Interactions
- S100 Proteins and Annexins
- Bone Tissue Engineering Materials
- Forensic and Genetic Research
- Dermatology and Skin Diseases
- Identification and Quantification in Food
- 3D Printing in Biomedical Research
- Food Allergy and Anaphylaxis Research
- Bacteriophages and microbial interactions
- Iron oxide chemistry and applications
- Advanced biosensing and bioanalysis techniques
- Nanoparticle-Based Drug Delivery
- Molecular Biology Techniques and Applications
- Chemical Synthesis and Characterization
- Chemical Analysis and Environmental Impact
- Supramolecular Self-Assembly in Materials
- Environmental DNA in Biodiversity Studies
- Neurological diseases and metabolism
Université de Bordeaux
2019-2024
Institut Polytechnique de Bordeaux
2020-2024
Centre National de la Recherche Scientifique
2011-2024
Chimie et Biologie des Membranes et des Nanoobjects
2020-2024
Institut Européen de Chimie et Biologie
2020-2024
Carnegie Institution for Science
2014-2022
Geophysical Laboratory
2014-2022
UCLouvain
2015-2020
Canadian Nautical Research Society
2019-2020
École Nationale Supérieure de Chimie, de Biologie et de Physique
2019-2020
ABSTRACT Staphylococcus aureus skin infection is a frequent and recurrent problem in children with the common inflammatory disease atopic dermatitis (AD). S. colonizes of majority AD exacerbates disease. The first step during colonization bacterial adhesion to cornified envelope corneocytes outer layer, stratum corneum. Corneocytes from are structurally different normal healthy skin. objective this study was identify proteins that promote adherence corneocytes. strains clonal complexes 1 8...
The development of bacterial biofilms on surfaces leads to hospital-acquired infections that are difficult fight. In Staphylococci, the cationic polysaccharide intercellular adhesin (PIA) forms an extracellular matrix connects cells together during biofilm formation, but molecular forces involved unknown. Here, we use advanced force nanoscopy techniques unravel mechanism PIA-mediated adhesion in a clinically relevant methicillin-resistant Staphylococcus aureus (MRSA) strain. Nanoscale...
forms biofilms on indwelling medical devices using a variety of cell-surface proteins. There is growing evidence that specific homophilic interactions between these proteins represent an important mechanism cell accumulation during biofilm formation, but the underlying molecular mechanisms are still not well-understood. Here we report direct measurement binding forces by serine-aspartate repeat protein SdrC and their inhibition peptide. Using single-cell single-molecule force measurements,...
ABSTRACT Bacterial pathogens that colonize host surfaces are subjected to physical stresses such as fluid flow and cell surface contacts. How bacteria respond mechanical cues is an important yet poorly understood issue. Staphylococcus aureus uses a repertoire of proteins resist shear stress during the colonization tissues, but whether their adhesive functions can be modulated by forces not known. Here, we show interaction S. clumping factor B (ClfB) with squamous epithelial envelope protein...
ABSTRACT The bacterial pathogen Staphylococcus aureus plays an important role in atopic dermatitis (AD), a chronic disorder that mostly affects children. Colonization of the skin AD patients by S. exacerbates disease, but molecular determinants bacterium-skin adhesive interactions are poorly understood. Specifically, reduced levels natural moisturizing factor (NMF) stratum corneum have been shown to be associated with more severe symptoms, whether this is directly related adhesion still open...
Staphylococcus aureus can invade various types of mammalian cells, thereby enabling it to evade host immune defenses and antibiotics. The current model for cellular invasion involves the interaction between bacterial cell surface located fibronectin (Fn)-binding proteins (FnBPA FnBPB) α5β1 integrin in membrane. While is believed that extracellular matrix protein Fn serves as a bridging molecule FnBPs integrins, fundamental forces involved are not known. Using single-cell single-molecule...
colonizes the skin of majority patients with atopic dermatitis (AD), and its presence increases disease severity. Adhesion
Due to an aging population, neurodegenerative diseases have become a major health issue, the most common being Alzheimer's disease. The mechanisms leading neuronal loss still remain unclear but recent studies suggest that soluble Aβ oligomers deleterious effects on membranes. Here, high-speed atomic force microscopy was used assess effect of oligomeric species variant Aβ1-42 amyloid peptide model membranes with various lipid compositions. Results showed does not interact composed...
Due to an aging population, neurodegenerative diseases such as Alzheimer's disease (AD) have become a major health issue. In the case of AD, Aβ1 - 42 peptides been identified one markers with formation senile plaques via their aggregation, and could play role in memory impairment other tragic syndromes associated disease. Many studies shown that not only morphology structure peptide assembly are playing important amyloid plaques, but also interactions between cellular membrane crucial...
The structural and mechanical properties of actin bundles are essential to eukaryotic cells, aiding in cell motility support the plasma membrane. Bundle formation occurs crowded intracellular environments composed various ions macromolecules. Although roles cations macromolecular crowding mechanics organization have been independently established, how changing both environmental conditions influence bundle at nanoscale has yet be established. Here we investigate electrostatics depletion...
The interactions between nucleic acids and mineral surfaces have been the focus of many studies in environmental sciences, biomedicine, as well origin life for prebiotic formation biopolymers. However, few focused on a wide range conditions likely modes attachment. Here we investigated adsorption ribonucleotides onto α-alumina over pH, ionic strength, ligand-to-solid ratio, by both an experimental theoretical approach. is strongly affected with maximum at pH values around 5. Alumina adsorbs...
A novel nanoscopy technique is developed for measuring the forces between bacterial pathogens and human skin at high spatiotemporal resolution. Applicable to a variety of microbes host cells, method offers exciting prospects understanding molecular details microbial infections.
The aim of this study is to investigate the impact stiffness and stress relaxation poly(acrylamide- co -acrylic acid) hydrogels on osteogenic differentiation human mesenchymal stem cells (hMSCs).
The adsorption of nucleic acid components onto the serpentinite-hosted hydrothermal mineral brucite has been investigated experimentally by determining equilibrium isotherms in aqueous solution. Thermodynamic characterization data performed using extended triple-layer model (ETLM) to establish a for stoichiometry and constants surface complexes. Infrared molecule–mineral complexes helped gain insight into molecular functional groups directly interacting with surface. Quantum mechanical...
In this study, we have applied Surface Enhanced Resonance Raman Scattering (SERRS) technology to the specific detection of DNA. We present an innovative SERRS sandwich-hybridization assay that allows DNA without any enzymatic amplification, such as is case with Polymerase Chain Reaction (PCR). some substrates, ancient or processed remains, amplification fails due alteration (degradation, chemical modification) presence inhibitors. Consequently, development a non-enzymatic method, allowing...
One of the hallmarks Alzheimer's disease (AD) is formation neurofibrillary tangles, resulting from aggregation tubulin associated unit protein (Tau), which holds a vital role in maintaining neuron integrity healthy brain. The development such aggregates and their deposition brain seem to correlate with onset neurodegeneration processes. misfolding subsequent into paired helical filaments that further form lead dysfunction neuronal loss cognitive decline. then seems be causative factor AD....
Ideal bone tissue engineering is to induce regeneration through the synergistic integration of biomaterial scaffolds, progenitor cells, and bone-forming factors. Biomimetic scaffolds imitate native extracellular matrix (ECM) are often utilized in vitro as analogues natural ECM facilitate investigations cell-ECM interactions processes. In vivo, cellular microenvironment has a crucial impact on regulating cell behavior functions. A PET surface was activated then functionalized with mimetic...
Understanding the basic mechanisms of bacterial sexuality is an important topic in current microbiology and biotechnology. While classical methods used to study gene transfer provide information on whole cell populations, nanotechnologies offer new opportunities for analyzing behavior individual mating partners. We introduce innovative atomic force microscopy (AFM) platform mechanically control DNA between single bacteria, focusing large conjugative pXO16 plasmid Gram-positive bacterium...