A5003580232- Force Microscopy Techniques and Applications
- Bacterial biofilms and quorum sensing
- Lipid Membrane Structure and Behavior
- Antimicrobial Resistance in Staphylococcus
- Polymer Surface Interaction Studies
- Biochemical and Structural Characterization
- Alzheimer's disease research and treatments
- Bacteriophages and microbial interactions
- Redox biology and oxidative stress
- Microfluidic and Bio-sensing Technologies
- thermodynamics and calorimetric analyses
- SARS-CoV-2 and COVID-19 Research
- Surface Modification and Superhydrophobicity
- Adhesion, Friction, and Surface Interactions
- Mechanical and Optical Resonators
- Protein Structure and Dynamics
- Pharmacological Receptor Mechanisms and Effects
- Near-Field Optical Microscopy
- Advanced Electron Microscopy Techniques and Applications
- Pickering emulsions and particle stabilization
- Cellular Mechanics and Interactions
- Amyotrophic Lateral Sclerosis Research
- Prion Diseases and Protein Misfolding
- Food composition and properties
- RNA Research and Splicing
UCLouvain
2013-2023
PRG S&Tech (South Korea)
2018
United States Virgin Islands Department of Health
2018
Institut des Arts de Diffusion
2018
Institute of Life Sciences
2016
Abstract Study of the interactions established between viral glycoproteins and their host receptors is critical importance for a better understanding virus entry into cells. The novel coronavirus SARS-CoV-2 cells mediated by its spike glycoprotein (S-glycoprotein), angiotensin-converting enzyme 2 (ACE2) has been identified as cellular receptor. Here, we use atomic force microscopy to investigate mechanisms which S-glycoprotein binds ACE2 We demonstrate, both on model surfaces living cells,...
AD (Alzheimer's disease) is linked to Abeta (amyloid beta-peptide) misfolding. Studies demonstrate that the level of soluble oligomeric forms correlates better with progression disease than fibrillar forms. Conformation-dependent antibodies have been developed detect either oligomers or fibrils, suggesting structural differences between these exist. Using conditions which yield well-defined Abeta-(1-42) we studied secondary structure species by ATR (attenuated total reflection)-FTIR...
Clumping factor A (ClfA), a cell-wall-anchored protein from Staphylococcus aureus, is virulence in various infections and facilitates the colonization of protein-coated biomaterials. ClfA promotes bacterial adhesion to blood plasma fibrinogen (Fg) via molecular forces that have not been studied so far. unique, yet poorly understood, feature its ability favor Fg at high shear stress. Unraveling strength dynamics ClfA-Fg interaction would help us better understand how S. aureus colonizes...
The development of bacterial biofilms on surfaces leads to hospital-acquired infections that are difficult fight. In Staphylococci, the cationic polysaccharide intercellular adhesin (PIA) forms an extracellular matrix connects cells together during biofilm formation, but molecular forces involved unknown. Here, we use advanced force nanoscopy techniques unravel mechanism PIA-mediated adhesion in a clinically relevant methicillin-resistant Staphylococcus aureus (MRSA) strain. Nanoscale...
forms biofilms on indwelling medical devices using a variety of cell-surface proteins. There is growing evidence that specific homophilic interactions between these proteins represent an important mechanism cell accumulation during biofilm formation, but the underlying molecular mechanisms are still not well-understood. Here we report direct measurement binding forces by serine-aspartate repeat protein SdrC and their inhibition peptide. Using single-cell single-molecule force measurements,...
Although bacterial pili are known to mediate cell adhesion a variety of substrates, the molecular interactions behind this process poorly understood. We report direct measurement forces guiding pili-mediated adhesion, focusing on medically important probiotic bacterium Lactobacillus rhamnosus GG (LGG). Using non-invasive single-cell force spectroscopy (SCFS), we quantify between individual bacteria and biotic (mucin, intestinal cells) or abiotic (hydrophobic monolayers) surfaces. On...
The surface of oxygen-plasma-treated polystyrene (PSox) was investigated using X-ray photoelectron spectroscopy (XPS), streaming potential measurements and a dynamic study the wetting properties at different pH (Wilhelmy plate method). PSox is functionalized with various oxygen-containing groups, including carboxyl functions, must be viewed as covered by polyelectrolyte which swells depending on pH. hysteresis, its evolution upon repeated cycles influence are controlled dissolution fragments...
Candida glabrata is an opportunistic human fungal pathogen which binds to surfaces mainly through the Epa family of cell adhesion proteins. While some proteins mediate specific lectin-like interactions with epithelial cells, others promote and biofilm formation on plastic via nonspecific that are not yet elucidated. We report measurement hydrophobic forces engaged in Epa6-mediated by means atomic force microscopy (AFM). Using single-cell spectroscopy, we found C. wild-type (WT) cells attach...
Abstract Study of virus entry into cells is critical importance for a better understanding the interactions established between viral glycoproteins and their receptors at cell surface could help to develop novel antiviral strategies. The coronavirus (SARS-CoV-2) host mediated by transmembrane spike glycoprotein (S-glycoprotein) angiotensin-converting enzyme 2 (ACE2) has been identified as cellular receptor. Here, we used atomic force microscopy investigate molecular mechanisms which S- binds...
Aggregates of TAR DNA-binding protein (TDP-43) are a hallmark several neurodegenerative disorders, including amyotrophic lateral sclerosis (ALS). Although TDP-43 aggregates an undisputed pathological species at the end stage these diseases, molecular changes underlying initiation aggregation not fully understood. The aim this study was to investigate how phase separation affects self-aggregation and seeded by pre-formed either low-complexity domain (LCD) or its short aggregation-promoting...
Macroscopic assays that are traditionally used to investigate the adhesion behaviour of microbial cells provide averaged information obtained on large populations and do not measure fundamental forces driving single-cell adhesion. Here, we use force spectroscopy (SCFS) quantify specific non-specific engaged in human fungal pathogen Candida albicans. Saccharomyces cerevisiae expressing C. albicans protein Als5p were attached atomic microscope tipless cantilevers using a bioinspired...
A novel nanoscopy technique is developed for measuring the forces between bacterial pathogens and human skin at high spatiotemporal resolution. Applicable to a variety of microbes host cells, method offers exciting prospects understanding molecular details microbial infections.
Natural rubber (NR) latex particles exhibit a core–shell structure, with polyisoprene elastomer as the core and thin layer made of proteins lipids shell. Knowledge surface properties interactions NR is key to gain detailed understanding their colloidal behaviour efficiently exploit them in industrial processes. Here, we report first nanoscale measurement stimuli-responsive single by atomic force microscopy (AFM), revealing that molecular are tightly controlled salt conditions....
Cellular membrane lateral organization, in particular the assembly of lipids domains, is difficult to evaluate at high resolution. Here, we used atomic force microscopy (AFM) investigate high-resolution lipid membranes containing variable amounts sphingomyelin (SM) and cholesterol (Chol), two abundant lipids. To this end, developed new AFM tip functionalization strategies specifically probe SM Chol. Multiparametric imaging allowed us highlight submicrometric organization these within...
Atomic force microscopy with chemically sensitive tips was used to investigate the hydrophobic and electrostatic interaction forces of a stimuli-responsive adhesive polymer, their dynamic changes in response water immersion salt concentration. Block copolymer-filled coatings were obtained by incorporating an amphiphilic block copolymer containing polydimethylsiloxane (PDMS) poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) PDMS matrix. Topographic images fresh samples revealed presence...
The pancreas is a complex organ consisting of differentiated cells and extracellular matrix (ECM) organized adequately to enable its endocrine exocrine functions. Although much known about the intrinsic factors that control development, very few studies have focused on microenvironment surrounding pancreatic cells. This environment composed various ECM components, which play critical role in maintaining tissue organization homeostasis. In this study, we applied mass spectrometry identify...
Abstract Transposon Tn4430 belongs to a widespread family of bacterial transposons, the Tn3 family, which plays prevalent role in dissemination antibiotic resistance among pathogens. Despite recent data on structural architecture transposition complex, molecular mechanisms underlying replicative these elements are still poorly understood. Here, we use force-distance curve-based atomic force microscopy probe binding TnpA transposase DNA molecules containing one or two transposon ends and...