- Polymer Surface Interaction Studies
- RNA Research and Splicing
- Electrostatics and Colloid Interactions
- Microfluidic and Bio-sensing Technologies
- Microfluidic and Capillary Electrophoresis Applications
- Blood properties and coagulation
- Surfactants and Colloidal Systems
- RNA modifications and cancer
- Nanofabrication and Lithography Techniques
- Protein purification and stability
- Lipid metabolism and biosynthesis
- RNA and protein synthesis mechanisms
- Advanced Biosensing Techniques and Applications
- Neurogenetic and Muscular Disorders Research
- Protein Structure and Dynamics
- Electrohydrodynamics and Fluid Dynamics
- Amyotrophic Lateral Sclerosis Research
- Microplastics and Plastic Pollution
- thermodynamics and calorimetric analyses
- Coagulation and Flocculation Studies
- Protein Interaction Studies and Fluorescence Analysis
- Heat shock proteins research
- Graphene and Nanomaterials Applications
- Molecular Junctions and Nanostructures
- Monoclonal and Polyclonal Antibodies Research
Jerzy Haber Institute of Catalysis and Surface Chemistry, Polish Academy of Sciences
2009-2023
Vlaams Instituut voor Biotechnologie
2019-2023
VIB-VUB Center for Structural Biology
2019-2023
Vrije Universiteit Brussel
2019-2023
UCLouvain
2017-2021
Polish Academy of Sciences
2006-2013
University College Dublin
2012
The rapidly-growing field of polymer stimuli-responsive materials (smart materials) resulted in many advances designing and developing multi-functional systems, novel architectures, new synthetic routes for different applications. smart with a wide range stimuli, such as pH, light, temperature, enzyme, redox, electric magnetic fields play an important role applications disciplines, including nanotechnology, biochemistry, medicine, science, engineering, etc. This review aims to provide...
Abstract Understanding the kinetics, thermodynamics, and molecular mechanisms of liquid–liquid phase separation (LLPS) is paramount importance in cell biology, requiring reproducible methods for studying often severely aggregation-prone proteins. Frequently applied approaches inducing LLPS, such as dilution protein from an urea-containing solution or cleavage its fused solubility tag, lead to very different kinetic behaviors. Here we demonstrate that at carefully selected pH values proteins...
In this work, the structure of poly(acrylic acid) (PAA) molecules in electrolyte solutions obtained from molecular dynamic simulations was compared with experimental data derived light scattering (PCS), viscosity, and electrophoretic measurements. Simulations measurements were carried out for polymer having a weight 12 kD various ionic strengths supporting (NaCl). The effect ionization degree polymer, regulated by change pH solution range 4-9 units, also studied systematically. It predicted...
Layer-by-layer (LbL) assembly is an attractive method for protein immobilization at interfaces, a much wanted step biotechnologies and biomedicine. Integrating proteins in LbL thin films however very challenging due to their low conformational entropy, heterogeneous spatial distribution of charges, polyampholyte nature. Protein–polyelectrolyte complexes (PPCs) are promising building blocks construction owing standardized charge polyelectrolyte (PE) corona. In this work, lysozyme was...
Aggregates of TAR DNA-binding protein (TDP-43) are a hallmark several neurodegenerative disorders, including amyotrophic lateral sclerosis (ALS). Although TDP-43 aggregates an undisputed pathological species at the end stage these diseases, molecular changes underlying initiation aggregation not fully understood. The aim this study was to investigate how phase separation affects self-aggregation and seeded by pre-formed either low-complexity domain (LCD) or its short aggregation-promoting...
Selective protein adsorption is a key challenge for the development of biosensors, separation technologies, and smart materials medicine biotechnologies. In this work, strategy was developed selective adsorption, based on use mixed polymer brushes composed poly(ethylene oxide) (PEO), protein-repellent polymer, poly(acrylic acid) (PAA), weak polyacid whose conformation changes according to pH ionic strength surrounding medium. A mixture lysozyme (Lyz), human serum albumin (HSA), fibrinogen...
The adsorption of fibrinogen on polystyrene latex particles was studied using the concentration depletion method combined with AFM detection residual protein after adsorption. Measurements were carried out for a pH range 3.5-11 and an ionic strength 10(-3)-0.15 M NaCl. First, bulk physicochemical properties particle suspension characterized this strength. zeta potential number uncompensated (electrokinetic) charges determined from microelectrophoretic measurements. It revealed that molecules...
The adsorption of human serum fibrinogen on polystyrene latex particles was studied using the microelectrophoretic and concentration depletion methods. Measurements were carried out for pH 3.5 an ionic strength range 10–3 to 0.15 M NaCl. electrophoretic mobility determined as a function amount adsorbed (surface concentration). A monotonic increase in (zeta potential) observed, indicating significant all strengths. No changes observed prolonged time periods, suggesting irreversibility...
A standard method of protein immobilization is proposed, based on the use protein-polyelectrolyte complexes (PPCs) as building blocks for layer-by-layer assembly. Thicker multilayers, with a higher polyelectrolyte fraction, are obtained PPCs compared to single molecules. Biological activity not only maintained, but specific also higher, demonstrated lysozyme-poly(styrene sulfonate) complexes. This attributed more hydrated state assemblies. new opens up perspectives biotechnology and...
Proteins at interfaces are a key for many applications in the biomedical field, biotechnologies, biocatalysis, food industry, etc. The development of surface layers that allow to control and manipulate proteins is thus highly desired. In previous works, we have shown mixed polymer brushes combining protein-repellent properties poly(ethylene oxide) (PEO) stimuli-responsive adsorption behavior poly(acrylic acid) (PAA) could be synthesized used achieve switchable protein adsorption. With...
Fibrinogen (Fb) adsorption on positively charged latex particles (average diameter of 800 nm) was studied using the microelectrophoretic and concentration depletion methods based AFM imaging. Monolayers were adsorbed from diluted bulk solutions at pH 7.4 an ionic strength in range 10(-3) to 0.15 M where fibrinogen molecules exhibited average negative charge. The electrophoretic mobility after controlled systematically measured. A monotonic decrease fibrinogen-covered observed for all...
Theoretical calculations and various experimental techniques were applied to determine funda-mental physicochemical characteristics of the RNA-binding protein low complexity domain (hnRNPA2 LCD), in sodium chloride solutions. The monomer size, cross-section area, dependence nominal charge on pH, its isoelectric point predicted. These theoretical data allowed one analyze interpret adsorption hnRNPA2 LCD molecules mica, which was investigated by streaming potential technique, polymer...
Abstract Motivation Proteins able to undergo liquid–liquid phase separation (LLPS) in vivo and vitro are drawing a lot of interest, due their functional relevance for cell life. Nevertheless, the proteome-scale experimental screening these proteins seems unfeasible, because besides being expensive time-consuming, LLPS is heavily influenced by multiple environmental conditions such as concentration, pH temperature, thus requiring combinatorial number experiments each protein. Results To...
Protein adsorption on solid surfaces provides either beneficial or adverse outcomes, depending the application. Therefore, desire to predict, control, and regulate protein different is a major concern in field of biomaterials. The most widely used surface modification approach prevent limit based use poly (ethylene oxide) (PEO). On other hand, amount adsorbed poly(2-(dimethylamine)ethyl methacrylate) (PDMAEMA) coatings can be regulated by pH ionic strength medium. In this work, ultra-thin...
Manipulation of high-density materials, such as crystals and liquid condensates, is great importance for many applications, including serial crystallography, structural molecular biology, chemistry, medicine. In this work, we describe an acoustic technique to focus harvest flowing condensates. Moreover, show, based on numerical simulations, that the waves can be used size-based particle (crystals, droplets, etc.) separation. This essential technological step in biological research, medical...