A5070458098- Force Microscopy Techniques and Applications
- Lipid Membrane Structure and Behavior
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Mechanical and Optical Resonators
- Erythrocyte Function and Pathophysiology
- Cardiomyopathy and Myosin Studies
- Molecular Junctions and Nanostructures
- Blood properties and coagulation
- Wnt/β-catenin signaling in development and cancer
- Cellular Mechanics and Interactions
- Bacteriophages and microbial interactions
- Graphene and Nanomaterials Applications
- thermodynamics and calorimetric analyses
- Cancer-related gene regulation
- Cancer Research and Treatments
- Hemoglobin structure and function
- Genetics and Neurodevelopmental Disorders
- Caveolin-1 and cellular processes
- Cell Adhesion Molecules Research
- Polymer Surface Interaction Studies
- Nanopore and Nanochannel Transport Studies
- Respiratory viral infections research
- Advanced biosensing and bioanalysis techniques
- Complement system in diseases
Spanish National Centre for Cardiovascular Research
2023-2025
UCLouvain
2017-2024
PRG S&Tech (South Korea)
2018
Institut des Arts de Diffusion
2018
United States Virgin Islands Department of Health
2018
Institute of Life Sciences
2017-2018
Instituto de Ciencia de Materiales de Madrid
2014-2017
Consejo Superior de Investigaciones Científicas
2014-2015
Abstract Study of the interactions established between viral glycoproteins and their host receptors is critical importance for a better understanding virus entry into cells. The novel coronavirus SARS-CoV-2 cells mediated by its spike glycoprotein (S-glycoprotein), angiotensin-converting enzyme 2 (ACE2) has been identified as cellular receptor. Here, we use atomic force microscopy to investigate mechanisms which S-glycoprotein binds ACE2 We demonstrate, both on model surfaces living cells,...
We present a procedure that allows reliable determination of the elastic (Young’s) modulus soft samples, including living cells, by atomic force microscopy (AFM). The standardized nanomechanical AFM (SNAP) ensures precise adjustment optical lever system, prerequisite for all kinds spectroscopy methods, to obtain values independent instrument, laboratory and operator. Measurements hydrogel samples with well-defined using different AFMs revealed uncertainties in deflection sensitivity...
Wnt signaling is key to many developmental, physiological, and disease processes in which cells seem able discriminate between multiple ligands. This selective recognition or "decoding" capacity has remained enigmatic because Wnt/Frizzled interactions are largely incompatible with monospecific recognition. Gpr124 Reck enable brain endothelial selectively respond Wnt7. We show that binds low micromolar affinity the intrinsically disordered linker region of Availability Reck-bound Wnt7 for...
Antibody-antigen (Ab-Ag) recognition is the primary event at basis of many biosensing platforms. In label-free biosensors, these events occurring solid-liquid interfaces are complex and often difficult to control technologically across smallest length scales down molecular scale. Here a molecular-scale technique, such as single-molecule force spectroscopy, performed areas real electrode functionalized for immunodetection an inflammatory cytokine, viz. interleukin-4 (IL4). The statistical...
Viral infection is an intricate process that requires the concerted action of both viral and host cell components. Entry viruses into cells initiated by interactions between proteins their surface receptors. Despite recent progress, molecular mechanisms underlying multistep reovirus entry are poorly understood. Using atomic force microscopy, we investigated how σ1 attachment protein binds to α-linked sialic acid (α-SA) JAM-A cell-surface We discovered initial binding α-SA favors a strong...
Reovirus infection requires the concerted action of viral and host factors to promote cell entry. After interaction reovirus attachment protein σ1 with cell-surface carbohydrates proteinaceous receptors, additional mediate virus internalization. In particular, β1 integrin is required for endocytosis virions following junctional adhesion molecule A (JAM-A) binding. While integrin-binding motifs in surface-exposed region capsid λ2 are thought interaction, evidence direct integrin-reovirus...
Large-scale molecular dynamics (MD) simulations and atomic force microscopy (AFM) in liquid are combined to characterize the adsorption of Immunoglobulin G (IgG) antibodies over a hydrophobic surface modeled with three-layer graphene slab. We consider explicitly water solvent, simulating systems massive sizes (up 770 000 atoms), for four different orientations. Protocols based on steered MD speed up protein diffusion stage enhance dehydration process long simulation times (>150 ns) order...
Abstract Study of virus entry into cells is critical importance for a better understanding the interactions established between viral glycoproteins and their receptors at cell surface could help to develop novel antiviral strategies. The coronavirus (SARS-CoV-2) host mediated by transmembrane spike glycoprotein (S-glycoprotein) angiotensin-converting enzyme 2 (ACE2) has been identified as cellular receptor. Here, we used atomic force microscopy investigate molecular mechanisms which S- binds...
Abstract Tumor cells exhibit altered cholesterol content. However, structural subcellular distribution and implication in cancer cell invasion are poorly understood mainly due to difficulties investigate both quantitatively qualitatively compare isogenic models. Here, using the MCF10A line series (non-tumorigenic MCF10A, pre-malignant MCF10AT malignant MCF10CAIa cells) as a model of breast progression highly invasive MDA-MB-231 which exhibits common TP53 mutation, we investigated if...
Red blood cells present a complex cell plasma membrane architecture with submicrometric organization leading to nanomechanical heterogeneities.
PIEZO1 ion channels are activated by mechanical stimuli, triggering intracellular chemical signals. Recent structural studies suggest that plasma membrane tension or local curvature changes modulate channel gating and activation. However, whether localization is governed gradients long-range perturbations across the cells still unclear. Here, we probe nanoscale of on red blood (RBCs) at high resolution (∼30 nm), report for first time existence submicrometric clusters in native conditions....
Tumor cells present profound alterations in their composition, structural organization, and functional properties. A landmark of cancer is an overall altered mechanical phenotype, which so far are linked to changes cytoskeletal regulation organization. Evidence exists that the plasma membrane (PM) also shows drastic its composition However, biomechanical characterization PM remains limited mainly due difficulties encountered investigate it a quantitative label-free manner. Here, properties...
Aims: Adult mammalian hearts have limited regenerative capacity due to the inability of cardiomyocytes proliferate, a major clinical hurdle in contemporary cardiology. The presence highly organized, contractile sarcomeres has long been considered an impediment for cardiomyocyte division. Indeed, sarcomere disassembly is crucial step complete cell cycle few situations where observed proliferate. However, whether can per se trigger re-entry remains unknown, possibility that we tested here....
Cellular membrane lateral organization, in particular the assembly of lipids domains, is difficult to evaluate at high resolution. Here, we used atomic force microscopy (AFM) investigate high-resolution lipid membranes containing variable amounts sphingomyelin (SM) and cholesterol (Chol), two abundant lipids. To this end, developed new AFM tip functionalization strategies specifically probe SM Chol. Multiparametric imaging allowed us highlight submicrometric organization these within...
Single molecule force spectroscopy has been applied to measure the unbinding forces and energies between a siRNA polyamidoamine dendrimers deposited on mica surface.
The interactions of a biodegradable scaffold with cells or living tissues depend on the time-evolution nanoscale properties scaffold. We present an in situ quantitative study early-stage swelling and degradation poly(lactic-co-glycolic acid) (PLGA). A novel metrology scheme based force microscopy measurements patterns PLGA nanostructures is developed to characterize evolution topography, volume nanomechanical properties. roughness show oscillating behaviour during first eight days immersion;...
Abstract A current challenge to produce effective therapeutics is accurately determine the location of ligand-biding site and characterize its properties. So far, mechanisms underlying functional activation cell surface receptors by ligands with a complex binding mechanism remain poorly understood due lack suitable nanoscopic methods study them in their native environment. Here, we elucidated ligand-binding human G protein-coupled C5a receptor (C5aR). We discovered for first time...
Silica nanoparticles (SiNP) trigger a range of innate immune responses in relevant essential organs, such as the liver and lungs. Inflammatory reactions, including NLRP3 inflammasome activation, have been linked to particulate materials; however, molecular mechanisms key actors remain elusive. Although many receptors, several scavenger were suggested participate SiNP cellular uptake, mechanistic evidence their role on immunity is lacking. Here we present an atomic force microscopy-based...
Familial hypobetalipoproteinemia is a metabolic disorder mainly caused by mutations in the apolipoprotein B gene. In its homozygous form it can lead without treatment to severe ophthalmological and neurological manifestations. contrast, heterozygous generally asymptomatic but associated with low risk of cardiovascular disease. Acanthocytes or thorny red blood cells (RBCs) are described for both forms However, those morphological changes poorly characterized their potential consequences RBC...