A5049102228- Bacterial Infections and Vaccines
- Pneumonia and Respiratory Infections
- Respiratory viral infections research
- Pneumocystis jirovecii pneumonia detection and treatment
- Antifungal resistance and susceptibility
- Infective Endocarditis Diagnosis and Management
- Fungal Infections and Studies
- Reproductive tract infections research
- Dermatology and Skin Diseases
- Streptococcal Infections and Treatments
- Influenza Virus Research Studies
- HIV/AIDS oral health manifestations
- SARS-CoV-2 and COVID-19 Research
- Antimicrobial Resistance in Staphylococcus
- Vaccine Coverage and Hesitancy
- Neonatal and Maternal Infections
- Protein Kinase Regulation and GTPase Signaling
- Ion channel regulation and function
- HIV-related health complications and treatments
- Viral Infections and Outbreaks Research
- HIV Research and Treatment
- Infectious Encephalopathies and Encephalitis
- Zoonotic diseases and public health
- Food Allergy and Anaphylaxis Research
- Ocular Surface and Contact Lens
Children's Health Ireland at Crumlin
2020-2024
Beaumont Hospital
2021
Temple Street Children's University Hospital
2004-2020
Trinity College Dublin
1993-2005
Royal College of Surgeons in Ireland
2003
Rotunda Hospital
2003
Bristol Royal Infirmary
1997
University of Bristol
1997
Dublin Dental University Hospital
1997
Summary: Atypical oral Candida isolates were recovered from 60 HIV-infected and three HIV-negative individuals. These organisms germ-tube-positive produced abundant chlamydospores which frequently arranged in triplets or contiguous pairs. They belonged to C. albicans serotype A had atypical carbohydrate assimilation profiles. Fingerprinting the genomic DNA of a selection these with albicans-specific probe 27A five separate oligonucleotides, homologous eukaryotic microsatellite repeat...
Streptococcus pneumoniae, Haemophilus influenzae, and Neisseria meningitidis, which are typically transmitted via respiratory droplets, leading causes of invasive diseases, including bacteraemic pneumonia meningitis, secondary infections subsequent to post-viral disease. The aim this study was investigate the incidence disease due these pathogens during early months COVID-19 pandemic.In prospective analysis surveillance data, laboratories in 26 countries territories across six continents...
BackgroundThe Invasive Respiratory Infection Surveillance (IRIS) Consortium was established to assess the impact of COVID-19 pandemic on invasive diseases caused by Streptococcus pneumoniae, Haemophilus influenzae, Neisseria meningitidis, and agalactiae. We aimed analyse incidence distribution these during first 2 years compared preceding pandemic.MethodsFor this prospective analysis, laboratories in 30 countries territories representing five continents submitted surveillance data from Jan...
Invasive meningococcal disease (IMD) is a major cause of bacterial meningitis and septicaemia although infection by some serogroups may be prevented through vaccination. We aimed to describe the epidemiology IMD in EU/EEA countries during 2004-2014 monitor serogroup- age-specific trends, compare country trends period C conjugate (MCC) vaccine introduction.We analysed surveillance data age, gender, serogroup, outcome. estimated percentage change annual notification rate (NR), using linear...
ABSTRACT Staphylococcus aureus skin infection is a frequent and recurrent problem in children with the common inflammatory disease atopic dermatitis (AD). S. colonizes of majority AD exacerbates disease. The first step during colonization bacterial adhesion to cornified envelope corneocytes outer layer, stratum corneum. Corneocytes from are structurally different normal healthy skin. objective this study was identify proteins that promote adherence corneocytes. strains clonal complexes 1 8...
Clinical isolates of Neisseria meningitidis with reduced susceptibility to penicillin G (intermediate isolates, Pen(I)) harbor alterations in the penA gene encoding binding protein 2 (PBP2). A 402-bp DNA fragment 3' half was sequenced from a collection 1,670 meningococcal clinical 22 countries that spanned 60 years. Phenotyping, genotyping, and determination MICs were also performed. total 139 different alleles detected 38 highly related, clustered together maximum-likelihood analysis...
Carriage rates of Staphylococcus aureus on affected skin in atopic dermatitis (AD) are approximately 70%. Increasing disease severity during flares and overall correlate with increased burden S. aureus. Treatment AD therefore often targets topical systemic antimicrobials. To determine whether antimicrobial sensitivities genetic determinants resistance differed isolates from the children healthy child nasal carriers. In this case–control study, we compared (n = 50) attending a hospital...
ABSTRACT PCR-based assays for the identification of Neisseria meningitidis serogroups 29E, X, and Z by detection specific regions ctrA gene are described. The specificities these were confirmed using A, B, C, H, W135, Y, nongroupable meningococcal isolates.
Phospholipases C are known to be important regulators of cellular processes but may also act as virulence factors pathogenic microbes. At least three genes in the genome human-pathogenic fungus Candida albicans encode phospholipases with conserved phospholipase (Plc) motifs. None deduced protein sequences contain N-terminal signal peptides, suggesting that these not secreted. In contrast its orthologue Sacharomyces cerevisiae , CaPLC1 seems an essential gene. However, a conditional mutant...
Summary: Phospholipase C (PLC) enzymes are essential in regulating several important cellular functions eukaryotes, including yeasts. In this study, PCR was used to identify a gene encoding PLC activity Candida albicans , using oligonucleotide primers complementary sequences highly conserved amino acid regions within the X domains of previously characterized eukaryotic phospholipase genes. The nucleotide sequence C. gene, CAPLC1 (2997 bp), determined from recombinant clone containing 132 A...
Neisseria meningitidis is a common cause of bacterial meningitis in children and young adults worldwide. The 4CMenB vaccine (Bexsero), developed to combat meningococcal serogroup B (MenB) disease, contains subcapsular antigens that may induce immunity against strains N. meningitidis, regardless serogroup. Owing differential levels expression peptide diversity across strains, the antigen typing system (MATS) was estimate potential MenB strain coverage 4CMenB. Prior introducing into routine...
ABSTRACT A carriage study was undertaken ( n = 112) to ascertain the prevalence of Neisseria spp. following eighth case invasive meningococcal disease in young children (5 46 months) and members a large extended indigenous ethnic minority Traveller family 123), typically associated with high-occupancy living conditions. Nested multilocus sequence typing (MLST) employed for specimen extracts. Isolates were genome sequenced then assembled de novo deposited into Bacterial Isolate Genome...
ABSTRACT We developed two Neisseria meningitidis multiplex PCR assays to be used consecutively that allow determination of the serogroup and capsular status A, B, C, 29E, W135, X, Y cnl-3 / cnl-1 -like-containing N. isolates by direct analysis amplicon size. These offer a rapid simple method serogrouping .
Between March 2010 and November 2013 eight laboratory-confirmed cases of serogroup B, invasive meningococcal disease (IMD) were identified in an extended Irish Traveller family across three Health Service Executive (HSE) areas Ireland. Cases aged between 5 46 months, either a cousin or sibling another case. All survived. Chemoprophylaxis was given to relevant nuclear members close contacts on each occasion, but failed prevent further cases. Neisseria meningitidis isolates from six highly...
Group B streptococcal isolates (n = 159) from the three Dublin maternity hospitals, were serotyped and analysed for bac, bca, hylB, pepB, rib genes. The serotype distribution of was Ia, 19.5%; Ib, 18.9%; II, 10.7%; III, 29.5%; IV, 1.9%; V, 15.1%; non-typeable, 4.4%. There a statistically significant association between invasive status (carriage or infection) (P < 0.005), but no degree invasiveness demonstrated. presence absence each gene not associated with isolates. Statistically...
AIM: To develop a polymerase chain reaction (PCR) based method to detect penicillin susceptibility in isolates of Streptococcus pneumoniae (SP). METHOD: PCR primers were designed amplify differential nucleotide sequences the penicillin-binding protein (PBP) genes 2b, 2x, and 1a susceptible resistant strains SP. Primers derived from PBP 2x 2b products S (PSSP), whereas gene (PRSP). RESULTS: Two hundred thirty clinical SP USA, UK, Kenya, Romania, Kingdom Saudi Arabia tested. Of isolates, 116...
We examined the epidemiology of invasive meningococcal disease (IMD) in Republic Ireland (ROI) between epidemiological year (EY) 1996/1997 and EY2015/2016. Over 20 EYs, 3707 cases were reported with annual incidence rates per 100 000 peaking at 11.6 EY1999/2000, decreasing significantly to 1.5 The highest burden was infants children <5, whereas adults aged ⩾65 years experienced case fatality ratio (CFR) 15.7% but over study period median CFR remained low (4.4%). Meningococcal serogroup B...