A5028541331- Bacterial Infections and Vaccines
- Pneumonia and Respiratory Infections
- SARS-CoV-2 and COVID-19 Research
- Respiratory viral infections research
- COVID-19 Clinical Research Studies
- Streptococcal Infections and Treatments
- Influenza Virus Research Studies
- Vector-borne infectious diseases
- SARS-CoV-2 detection and testing
- Xenotransplantation and immune response
- Microbial infections and disease research
- Oral and gingival health research
- COVID-19 and Mental Health
- Viral Infections and Vectors
- Oral and Maxillofacial Pathology
- Antimicrobial Resistance in Staphylococcus
- Bacterial Identification and Susceptibility Testing
- Antibiotic Resistance in Bacteria
- Immunotherapy and Immune Responses
- Clostridium difficile and Clostridium perfringens research
- Escherichia coli research studies
- Mosquito-borne diseases and control
- Infection Control and Ventilation
- Platelet Disorders and Treatments
- Menopause: Health Impacts and Treatments
Queen Mary Hospital
2025
University of Hong Kong
2025
University of Würzburg
2011-2024
Institute of Medical Microbiology and Hygiene
2015-2024
Institut für Hygiene und Umwelt
2011
Stanford University
2002-2005
Yale University
1994-2004
University of Freiburg
2003-2004
Johns Hopkins University
1997
Johns Hopkins Medicine
1997
Streptococcus pneumoniae, Haemophilus influenzae, and Neisseria meningitidis, which are typically transmitted via respiratory droplets, leading causes of invasive diseases, including bacteraemic pneumonia meningitis, secondary infections subsequent to post-viral disease. The aim this study was investigate the incidence disease due these pathogens during early months COVID-19 pandemic.In prospective analysis surveillance data, laboratories in 26 countries territories across six continents...
BackgroundThe Invasive Respiratory Infection Surveillance (IRIS) Consortium was established to assess the impact of COVID-19 pandemic on invasive diseases caused by Streptococcus pneumoniae, Haemophilus influenzae, Neisseria meningitidis, and agalactiae. We aimed analyse incidence distribution these during first 2 years compared preceding pandemic.MethodsFor this prospective analysis, laboratories in 30 countries territories representing five continents submitted surveillance data from Jan...
We report the cloning and characterization of two outer surface proteins (Osps), designated OspE OspF, from strain N40 Borrelia burgdorferi, spirochetal agent Lyme disease. The ospE ospF genes are structurally arranged in tandem as one transcriptional unit under control a common promoter. gene, located at 5' end operon, is 513 nucleotides length encodes 171-amino-acid protein with calculated molecular mass 19.2 kDa. 27 bp downstream stop codon consists 690 230 amino acids 26.1 Pulsed-field...
We determined whether Borrelia burgdorferi outer surface proteins (Osps) E and F could elicit immune responses useful for a Lyme disease vaccine. Thirty days after challenge with B. burgdorferi, mice produced antibodies to OspE but not OspF, whereas OspF were present in sera of obtained 90 infection. Examination from patients revealed small number (14%) early-stage majority (58%) late-stage disease, while rarely detected patients. Mice immunized recombinant or high titers respectively....
Abstract Oestrogen is known to influence pyramidal cell spine synapse plasticity in the CA1 subfield of hippocampus. Apart from direct oestrogen action on hippocampus, effects mediated by subcortical structures are be important. The purpose this study was investigate whether medial septum diagonal band Broca (MSDB) takes part mediating Special attention given role cholinergic MSDB neurons that project as a rather large population them contains receptors and, consequently, may sensitive...
The aim of this study was to investigate the jawbone concentration clindamycin (CLI) in patients with an osteonecrosis jaw (ONJ). Patients medication-related ONJ [MRONJ] and osteoradionecrosis [ORN]) antibiotic treatment CLI were included. Plasma, vital necrotic bone samples collected. Plasma analyzed by liquid chromatography–tandem mass spectrometry. MRONJ exhibited a mean plasma 9.6 μg/mL (SD ± 3.6 μg/mL) concentrations 2.3 μg/g 1.4 μg/g) 2.1 2.4 samples, without statistical significance...
ABSTRACT Staphylococcus aureus is a major cause of variety both local and systemic infections. It can invade human host cells, process that may account for disseminated recurrent S. postinvasion events in nonprofessional phagocytes are only partially understood. While morphological data suggest phagosomal escape, there lack corroborating functional data. Using combination pH determination techniques, we have tested the integrity -containing phagosomes 293 (HEK-293), HeLa, EA.hy926 cells over...
ABSTRACT Staphylococcus aureus reacts to changing environmental conditions such as heat, pH, and chemicals through global regulators the sae ( S. exoprotein expression) two-component signaling system. Subinhibitory concentrations of some antibiotics were shown increase virulence factor expression. Here, we investigated stress response sublethal a commonly used biocide (Perform), by real-time quantitative PCR (qRT-PCR), promoter activity assay, sodium dodecyl sulfate (SDS)-polyacrylamide gel...
Against the background of current COVID-19 infection dynamics with its rapid spread SARS-CoV-2 variants concern (VOC), immunity and vaccine prevention healthcare workers (HCWs) against continues to be high importance. This observational cross-section study assesses factors influencing level anti-SARS-CoV-2-spike IgG after or vaccination. One thousand seven hundred fifty HCWs were recruited meeting following inclusion criteria: age ≥18 years, PCR-confirmed convalescence and/or at least one...
Intracellular Staphylococcus aureus has been implicated in the establishment of chronic infections. It is therefore imperative to understand by what means S. able survive within cells. Here we use two expression systems with a fluorescent readout assay alpha-toxin and function phagolysosomes infected upper-airway epithelial cells: avirulent carnosus TM300 phenotypically alpha-toxin-negative laboratory strains. Data from CFU recovery assays suggest that presence not beneficial for...
Objective The aim of this study was to determine the prevalence Neisseria meningitidis, Haemophilus influenzae, Streptococcus pneumoniae, group A (GAS), and Staphylococcus aureus in asymptomatic elderly people unravel risk factors leading colonization. Methods multi-centre cross-sectional conducted including 677 adults aged 65 years or more, living at home nursing homes. Study areas were Greater Aachen (North-Rhine-Westphalia) Wuerzburg (Bavaria), both regions with medium high population...
Mechanisms of wound healing are often impaired in patients with osteonecrosis the jaw (ONJ). According to guidelines for treatment this disease, early surgical intervention is indicated. However, surgery faces complications such as disorders. The application platelet-rich fibrin (PRF) after necrosectomy between bone and mucosa may constitute a promising approach improve results. An aspect that was not investigated until now PRF acts "bio-carrier" antibiotics previously applied intravenously.
Abstract Objectives Recent research has demonstrated that platelet-rich fibrin (PRF) is an appropriate carrier for ampicillin/sulbactam. The aim of the study was to investigate whether PRF also a suitable bio-carrier clindamycin (CLI). Methods membranes were produced from 36 patients receiving intravenous therapy with CLI (e.g. due diagnosis osteonecrosis jaw or infections). Concentrations in measured liquid chromatography-tandem mass spectrometry, and antimicrobial effects investigated...
Introduction Uncomplicated urinary tract infections (uUTIs) in women are common encountered primary care. Evidence suggests that rapid point-of-care tests (POCTs) to detect bacteria and erythrocytes urine at presentation may help care clinicians identify with uUTIs whom antibiotics can be withheld without influencing clinical outcomes. This pilot study aims provide preliminary evidence on whether a POCT informed management of uUTI safely reduce antibiotic use. Methods analysis is an...
Different platelet-rich fibrin (PRF) protocols exist and are known to differ in resulting mechanical bioactive properties. Centrifugation parameters may also influence drug release, particular antibiotics, when using PRF as a bio-carrier. We thus evaluated three common regarding effects on the bio-carrier properties.In prospective trial comprising 33 patients, we compared different for ampicillin/sulbactam (SAM). Blood samples were taken shortly after single dose of (2 g/1 g) was...