A5081974588- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- Xenotransplantation and immune response
- Pluripotent Stem Cells Research
- Gut microbiota and health
- Banana Cultivation and Research
- Virus-based gene therapy research
- Diet and metabolism studies
- Viral Infectious Diseases and Gene Expression in Insects
- Date Palm Research Studies
- RNA Interference and Gene Delivery
- Probiotics and Fermented Foods
- Cancer Research and Treatments
- Oil Palm Production and Sustainability
- Insect behavior and control techniques
- Chromosomal and Genetic Variations
- Insect Resistance and Genetics
- Cassava research and cyanide
- Agricultural and Food Sciences
- Amyotrophic Lateral Sclerosis Research
- Effects of Environmental Stressors on Livestock
- Birth, Development, and Health
- Allelopathy and phytotoxic interactions
- Insect Pest Control Strategies
- Bone Metabolism and Diseases
Università Cattolica del Sacro Cuore
2013-2024
Humanitas University
2018
Albert Einstein College of Medicine
2018
Institute of Genetic and Biomedical Research
2018
Creative Research Enterprises (United States)
2018
University of California, Santa Cruz
2013
Tecnologie Avanzate (Italy)
1993-1994
Institute of Biomedical Technologies
1993-1994
National Research Council
1992
Joint Research Centre
1992
VEGF coordinates complex regulation of cellular regeneration and interactions between endothelial perivascular cells; dysfunction the signaling system leads to retinopathy. Here, we show that systemic delivery placental growth factor (PlGF) by protein implantation, tumors, adenoviral vectors ablates pericytes from mature retinal vasculature through receptor 1 (VEGFR1)-mediated pathway, leading increased vascular leakage. In contrast, demonstrate 2 (VEGFR2) is primarily expressed in...
Interest in glypican-3 (GPC3), a member of the glypican-related integral membrane heparan sulfate proteoglycans (GRIPS) family, has increased with finding that it is mutated Simpson-Golabi-Behmel overgrowth syndrome (Pilia et al. [1996] Nat. Genet. 12:241–247). The working model suggested membrane-bound protein acts locally to limit tissue and organ growth may function by interacting insulin-like factor 2 (IGF2) its local effective level. Here we have tested two predictions model. In situ...
Abstract We report three novel osteopetrosis patients with OSTM1 mutations and review two that have been previously described. Our analysis suggests defines a new subset of severe central nervous system involvement. This defect is also present in the gl mouse, which could represent good model to study role gene pathogenesis this disease. Introduction: Autosomal recessive (ARO) hereditary bone disease whose cellular basis osteoclast, but heterogeneous molecular defects. In addition TCIRG1...
Animal studies show that high fat (HF) diet-induced gut microbiota contributes to the development of obesity. Oil composition high-fat diet affects metabolic inflammation differently with deleterious effects by saturated fat. The aim present study was examine diversity and capacity cecal bacterial community in C57BL/6 N mice administered two different diets, enriched respectively coconut oil (HFC, fat) or soy (HFS, polyunsaturated fat). relative impact each hypercaloric evaluated after 2 8...
Polyphenols are multifaceted bioactive compounds, but little is known about their real impact on human health after consumption. In this work, the phenolic profiling of quebracho, yellow maize, and violet rice extracts was comprehensively investigated, together with in vitro digestion colonic fermentation bioaccessibility bioavailability these phytochemicals. The different matrices showed distinct profiles, potentially influencing starch under cooking conditions. Furthermore, underwent...
The underlying mechanism by which anti-VEGF agents prolong cancer patient survival is poorly understood. We show that in a mouse tumor model, VEGF systemically impairs functions of multiple organs including those the hematopoietic and endocrine systems, leading to early death. Anti-VEGF antibody, bevacizumab, receptor 2 (VEGFR-2), but not anti-VEGFR-1, reversed VEGF-induced cancer-associated systemic syndrome (CASS) prevented death tumor-bearing mice. Surprisingly, VEGFR2 blockage improved...
Recently, CD40L has been identified as the gene responsible for X chromosome-linked hyper-IgM syndrome (HIGM1). on activated T cells from HIGM1 patients fails to bind B-cell CD40 molecules, and subsequent analysis of transcripts by reverse transcription PCR demonstrated coding region mutations in these patients. This approach, however, is limited use prenatal diagnosis early-gestation fetus. In this report, we have defined genomic structure gene, which composed five exons four intervening...
The pig represents the xenogeneic donor of choice for future organ transplantation in humans anatomical and physiological reasons. However, to bypass several immunological barriers, strong stable human genes expression must occur pig's organs. In this study we created transgenic pigs using vitro transfection cultured cells combined with somatic cell nuclear transfer (SCNT) evaluate ubiquitous transgene driven by pCAGGS vector presence different selectors. confirmed be a very effective...
The synthesis of heterologous proteins in lactobacilli is strongly influenced by the promoter selected for expression. In addition, activity promoters themselves may vary among different bacterial hosts. Three were investigated their capability to drive enhanced green fluorescent protein (EGFP) expression Lactococcus lactis spp. cremoris MG1363, Lactobacillus reuteri DSM 20016(T) and five L. strains isolated from chicken crops. acidophilus surface layer gene (slp), lactate dehydrogenase...
Somatic cell nuclear transfer (SCNT) was first developed in livestock for the purpose of accelerating widespread use superior genotypes. Although many problems still exist now after fifteen years research owing to limited understanding genome reprogramming, SCNT has provided a powerful tool make copies selected individuals different species, study pluripotency and differentiation, opening new avenues regenerative medicine representing main route making transgenic livestock. Besides...
A total of 60 Lactobacillus sp. strains were examined for expression auto-aggregation and cell surface hydrophobicity. Isolates obtained from the vagina healthy women (n=20). The results showed that occurrence hydrophobicity correlated with auto-aggregative activity in 12 homofermentative strains. aggregation mechanism was mediated by presence an promoting factor (APF) one strain, HV 142. APF confirmed DNA hybridisation a 1.3 kb PstI fragment recombinant plasmid pFDS containing reading open...
Lactobacillus gasseri 4B2 is a human isolate characterised by strong autoaggregating phenotype mediated APF (aggregation-promoting factor), secreted protein. Two primer pairs were developed for simultaneous amplification of specific fragment the gene and highly conserved region 16S rRNA gene. The specificity this protocol was checked in DNA samples isolated from single mixed cultures Lactobacillus. same successfully used directly adding whole bacterial cells to PCR reaction tubes....
Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease that occurs in two clinically indistinguishable forms: sporadic (SALS) and familial (FALS), the latter linked to several gene mutations, mostly inheritable dominant manner. Nearly 20% of FALS forms are mutations Cu/Zn superoxide dismutase (SOD1) gene. Research on ALS relies transgenic models particularly mice carrying glycine-to-alanine conversion at 93rd codon (G93A) hSOD1 Although G93A have been widely employed...
Artichoke is a relevant source of health-promoting compounds such as polyphenols and sesquiterpene lactones. In this study, the bioaccessibility gut bioavailability artichoke constituents were evaluated by combining in vitro digestion large intestine fermentation, metabolomics, Caco-2 human intestinal cells model. Moreover, ability to modulate starch digestibility was also explored. An untargeted metabolomic approach based on liquid chromatography quadrupole-time-of-flight (UHPLC/QTOF) mass...
The gut microbiota has been shown to be involved in host energy homeostasis and diet-induced metabolic disorders. To gain insight into the relationships among diet, host, we evaluated effects of a high-fat (HF) diet on bacterial community weaning mice. C57BL/6 mice were fed either control or enriched with soy oil for 1 2 weeks. Administration HF caused an increase plasma total cholesterol levels, while no significant differences body weight observed between two diets. Denaturing gradient gel...
The clustered regularly interspaced short palindromic repeat (CRISPR)/associated 9 (Cas9) technology has been recently added to the tools allowing efficient and easy DNA targeting, representing a very promising approach gene engineering. Using CRISPR/Cas9 system we have driven integration of exogenous sequences X-linked Hprt mouse embryonic stem cells. We show here that simple fluorescence in situ hybridization (FISH)-based strategy allows detection frequency evaluation non-specific...
Cell fusion between neoplastic and normal cells has been suggested to play a role in the acquisition of malignant phenotype. Several studies have pointed macrophage as partner this fusion, suggesting that fused could acquire new invasive properties become able disseminate distant organs. However, conclusion is mainly based on with transplantable cell lines. We tested occurrence MMTV-neu model mouse mammary carcinoma. In first approach, we generated aggregation chimeras GFP/neu RFP/neu...