Summary Ripe tomato fruits accumulate large amounts of the red linear carotene, lycopene (a dietary antioxidant) and small its orange cyclisation product, beta‐carotene (pro‐vitamin A). Lycopene is transformed into by action beta‐cyclase (β‐Lcy). We introduced, via Agrobacterium ‐mediated transformation, DNA constructs aimed at up‐regulating (OE construct) or down‐regulating (AS expression β‐Lcy gene in a fruit‐specific fashion. Three transformants containing OE construct show significant...

ABSTRACT The high-yield expression of a neutralizing epitope from human immunodeficiency virus type 1 (HIV-1) on the surface plant and its immunogenicity are presented. highly conserved ELDKWA glycoprotein (gp) 41 was expressed as an N-terminal translational fusion with potato X (PVX) coat protein. resulting chimeric particles (CVPs), purified used to immunize mice intraperitoneally or intranasally, were able elicit high levels HIV-1-specific immunoglobulin G (IgG) IgA antibodies....

Summary The aims of this work were to obtain a human antibody against the tumour‐associated antigen tenascin‐C (TNC) and compare yield quality plant‐produced in either stable transgenics or using transient expression system. To end, characterization full‐sized immunoglobulin G (IgG) [monoclonal H10 (mAb H10)], derived from selected single‐chain variable fragment (scFv) produced plants, is presented. mAb gene was engineered for plant expression, Nicotiana tabacum transgenic lines expressing...

In recent years, different HIV antigens have been successfully expressed in plants by either stable transformation or transient expression systems. Among proteins, Nef is considered a promising target for the formulation of multi-component vaccine due to its implication first steps viral infection. Attempts express as single protein product (not fused stabilizing protein) transgenic resulted disappointingly low yields (about 0.5% total soluble protein). this work we describe system based on...

The potexvirus Potato virus X (PVX) can be modified genetically to generate chimeric particles (CVPs) carrying heterologous peptides fused coat protein (CP) subunits. A spontaneous PVX mutant expressing a truncated, but functional, form of the CP has been isolated. With aim exploiting this display useful for vaccine formulations, two novel viral expression vectors based on pPVX201 (bearing wild-type genome) were constructed encoding truncated CP. Both able produce infectious in planta and...

Plants may represent excellent alternatives to classical heterologous protein expression systems, especially for the production of biopharmaceuticals and vaccine components. Modern vaccines are becoming increasingly complex, with incorporation multiple antigens. Approaches towards developing an HIV appear confirm this, a combination candidate Among these, HIV-Nef is considered promising target development because immune responses directed against this viral could help control initial steps...

The overall quality of recombinant IgG antibodies in plants is dramatically compromised by host endogenous proteases. Different approaches have been developed to reduce the impact proteolysis on IgGs, notably involving site-directed mutagenesis eliminate protease-susceptible sites or situ mitigation protease activities minimize antibody processing cell secretory pathway. We here characterized degradation profile H10, a human tumour-targeting monoclonal IgG, leaves Nicotiana benthamiana also...

Abstract Hairy root (HR) cultures derived from Agrobacterium rhizogenes transformation of plant tissues are an advantageous biotechnological manufacturing platform due to the accumulation recombinant proteins in otherwise largely protein free culture medium. In this context, HRs descending transgenic Nicotiana tabacum plants were successfully used for production several functional mAbs with plant‐type glycans. Here, we expressed tumor‐targeting monoclonal antibody mAb H10 obtained either by...

Biomaterials research for the discovery of new generation nanoparticles is one most active areas nanotechnoloy. In search nature-made nanometer-sized objects, plant virus particles appear as symmetrically defined entities that can be formed by protein self-assembly. particular, in field virology, there plenty literature available describing exploitation viral cages to produce safe vaccine vehicles and drug delivery. this context, we have investigated on use artichoke mottled crinkle (AMCV)...

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has killed more than 37,000 people in Italy and caused widespread socioeconomic disruption. Urgent measures are needed to contain control the virus, particularly diagnostic kits for detection surveillance, therapeutics reduce mortality among severely affected, vaccines protect remaining population. Here we discuss potential role of plant molecular farming rapid scalable supply protein antigens as reagents vaccine candidates,...

Classical Swine Fever (CSFV) is one of the most important viral infectious diseases affecting wild boars and domestic pigs. The etiological agent disease CSF virus, a single stranded RNA virus belonging to family Flaviviridae. All preventive measures in pigs have been focused interrupting chain infection avoiding spread CSFV within as well transmission from use plant based vaccine against would be advantageous organs can distributed without need particular treatments such refrigeration...

We have recently described an efficient transient expression system mediated by Agrobacterium tumefaciens for the production of HIV-1 Nef protein in Nicotiana benthamiana plants. In order to enhance yield recombinant we assayed effect three gene- silencing viral suppressor proteins (P25 Potato Virus X, P19 Artichoke Mottled Crinckle virus and Tomato Bushy Stunt virus) on levels. Results demonstrated that AMCV-P19 gave highest (1.3% total soluble protein) this was correlated a remarkable...

Anti-CD20 recombinant antibodies are among the most promising therapeutics for treatment of B-cell malignancies such as non-Hodgkin lymphomas. We recently demonstrated that an immunocytokine (2B8-Fc-hIL2), obtained by fusing anti-CD20 scFv-Fc antibody derived from C2B8 mAb (rituximab) to human interleukin 2 (hIL-2), can be efficiently produced in Nicotiana benthamiana plants. The purified (IC) bearing a typical plant protein N-glycosylation profile showed CD20 binding activity comparable...

We have recently characterized the degradation profiles of 2 human IgG1 monoclonal antibodies, tumor-targeting mAb H10 and anti-HIV 2G12. Both mAbs were produced in plants either as stable transgenics or using a transient expression system based on leaf agroinfiltration. The purified antibodies separated by 1DE protein bands N-terminal sequencing. proteolytic cleavage sites identified heavy chain (HC) both localized 3 inter-domain regions, suggesting that number events taking place is...

Summary Anti‐ CD 20 murine or chimeric antibodies ( A bs) have been used to treat non‐ H odgkin lymphomas NHL s) and other diseases characterized by overactive dysfunctional B cells. Abs demonstrated be effective in inducing regression of ‐cell lymphomas, although many cases patients relapse following treatment. promising approach improve the outcome m b therapy is use anti‐ deliver cytokines tumour microenvironment. In particular, IL ‐2‐based immunocytokines shown enhanced antitumour...

Hairy root (HR) cultures represent an attractive platform for the production of heterologous proteins, due to possibility secreting molecule interest in culture medium. The main limitation is low accumulation yields proteins. aim this study enhance a tumor-targeting antibody with human-compatible glycosylation profile HR To aim, authors produce Nicotiana benthamiana expressing red fluorescent protein (RFP) easily screen different auxins able induce secretion hormone 2,4-dichlorophenoxyacetic...

Monoclonal antibodies are considered to be highly effective therapeutic tools for the treatment of mild moderate COVID-19 patients. In present work, we describe production two SARS-CoV-2 human IgG1 monoclonal recognizing spike protein receptor-binding domain (RBD) and endowed with neutralizing activity (nAbs) in plants. The first one, mAbJ08-MUT, was previously isolated from a convalescent patient Fc-engineered prolong half-life reduce risk antibody-dependent enhancement. This nAb produced...

Infectious Bursal Disease Virus (IBDV), the etiological agent of Gumboro disease, causes mortality and immunosuppression in chickens major losses to poultry industry worldwide. The IBDV capsid protein VP2 is considered best candidate for production novel subunit vaccines. This structural contains conformational epitopes responsible induction neutralizing antibodies has been demonstrated able form supramolecular structures yeast insect cells. aim this study was express an engineered version...

Celiac disease is an immune-mediated disorder caused by the ingestion of gluten proteins. The gluten-free diet currently only therapy to achieve symptoms' remission. Biotechnological approaches are being explored obtain safer and healthier food for celiacs. This article analyzes consumer awareness acceptance advanced biotechnologies develop products. An online snowball sampling questionnaire was proposed 511 Italian participants, selected among celiac non-celiac people, from December 2020...