A5034252059- Plant tissue culture and regeneration
- Polysaccharides and Plant Cell Walls
- Mosquito-borne diseases and control
- Insect symbiosis and bacterial influences
- Monoclonal and Polyclonal Antibodies Research
- Transgenic Plants and Applications
- Plant pathogens and resistance mechanisms
- Glycosylation and Glycoproteins Research
- Cynara cardunculus studies
- Plant biochemistry and biosynthesis
- Plant Reproductive Biology
- Bacteriophages and microbial interactions
- Plant Molecular Biology Research
- Horticultural and Viticultural Research
- Plant Genetic and Mutation Studies
- Fermentation and Sensory Analysis
- Light effects on plants
- Postharvest Quality and Shelf Life Management
- Magnetic and Electromagnetic Effects
- Toxin Mechanisms and Immunotoxins
- Plant Virus Research Studies
- Photosynthetic Processes and Mechanisms
- Botanical Studies and Applications
- Insect and Pesticide Research
- Legume Nitrogen Fixing Symbiosis
National Agency for New Technologies, Energy and Sustainable Economic Development
2010-2024
ENEA Casaccia Research Centre
2007-2021
University of Verona
2010
Scuola Superiore Sant'Anna
2010
University of Cambridge
2010
Istituto per il Sistema Produzione Animale in Ambiente Mediterraneo
2008
National Research Council
2008
Innova (Italy)
1999
Sapienza University of Rome
1992-1997
Abstract The analysis of grapevine (Vitis vinifera) berries at the transcriptomic, proteomic, and metabolomic levels can provide great insight into molecular events underlying berry development postharvest drying (withering). However, large very different data sets produced by such investigations are difficult to integrate. Here, we report identification putative stage-specific biomarkers for withering and, our knowledge, first integrated systems-level study these processes. Transcriptomic,...
Summary Polygalacturonase‐inhibiting protein (PGIP) is a cell wall purified from hypocotyls of true bean ( Phaseolus vulgaris L.). PGIP inhibits fungal endopolygalacturonases and considered to be an important factor for plant resistance phytopathogenic fungi (Albersheim Anderson, 1971; Cervone et al. , 1987). The amino acid sequences the N‐terminus one internal tryptic peptide P. cv. Pinto were used design redundant oligonucleotides that successfully utilized as primers in polymerase chain...
The mosquito Aedes albopictusi is a competent vector of harmful human pathogens, including viruses causing dengue and chikungunya. Cytoplasmic incompatibility (CI) induced by endosymbiotic Wolbachia can be used to produce functionally sterile males that released in the field as suppression tool against this mosquito. Because available sexing methods are not efficient enough avoid unintentional release few transinfected females, we assessed CI pattern crosses between wPip...
The pgip-1 gene of Phaseolus vulgaris, encoding a polygalacturonase-inhibiting protein (PGIP), PGIP-1 (P. Toubart, A. Desiderio, G. Salvi, F. Cervone, L. Daroda, De Lorenzo, C. Bergmann, Darvill, and P. Albersheim, Plant J. 2:367–373, 1992), was expressed under control the cauliflower mosaic virus 35S promoter in tomato plants via Agrobacterium tumefaciens-mediated transformation. Transgenic with different expression levels were used infection experiments pathogenic fungi Fusarium oxysporum...
Summary The aims of this work were to obtain a human antibody against the tumour‐associated antigen tenascin‐C (TNC) and compare yield quality plant‐produced in either stable transgenics or using transient expression system. To end, characterization full‐sized immunoglobulin G (IgG) [monoclonal H10 (mAb H10)], derived from selected single‐chain variable fragment (scFv) produced plants, is presented. mAb gene was engineered for plant expression, Nicotiana tabacum transgenic lines expressing...
The practice of postharvest withering is commonly used to correct quality traits and sugar concentration high wines. To date, changes in the metabolome during berry maturation process have been well documented; however, biological events which occur at protein level yet be fully investigated. gain insight into process, we studied expression profiles grape (Corvina variety) development focusing on utilizing a two-dimensional differential gel electrophoresis (2D-DIGE) proteomics approach....
Among the strategies targeting vector control, exploitation of endosymbiont Wolbachia to produce sterile males and/or invasive females with reduced competence seems be promising. A new Aedes albopictus transinfection (ARwP-M) was generated by introducing wMel in ARwP line which had been established previously replacing wAlbA and wAlbB wPip strain. Various infection fitness parameters were studied comparing ARwP-M, wild-type (SANG population) Ae. sharing same genetic background. Moreover,...
The expression of exogenous antibodies in plant is an effective strategy to confer protection against viral infection or produce molecules with pharmaceutical interest. However, the acceptance transgenic technology obtain self-protecting plants depends on assessment their substantial equivalence compared non-modified crops established history safe use. In fact, possibility exists that introduction transgenes may alter endogenous genes and/or normal production metabolites. this study, we...
A better understanding of locally advanced cervical cancer (LACC) is mandatory for further improving the rates disease control, since a significant proportion patients still fail to respond or undergo relapse after concurrent chemoradiation treatment (CRT), and survival these has generally remained poor. To identify specific markers CRT response, we compared pretreatment biopsies from LACC with pathological complete response (sensitive) those showing macroscopic residual tumor (resistant)...
Introduction The future of human space missions relies on the ability to provide adequate food resources for astronauts and also reduce stress due environment (microgravity cosmic radiation). In this context, microgreens have been proposed astronaut diet because their fast-growing time high levels bioactive compounds nutrients (vitamins, antioxidants, minerals, etc.), which are even higher than mature plants, usually consumed as ready-to-eat vegetables. Methods Our study aimed identify best...
Releasing considerable numbers of radiation-sterilized males is a promising strategy to suppress mosquito vectors. However, releases may also include small percentages biting females, which translate non-negligible when are large. Currently, the effects irradiation on host-seeking and host-biting behaviors have not been exhaustively investigated. Information lacking regarding sterilizing treatment endosymbiotic bacterium Wolbachia, known affect vector competence infected mosquitos.To...
Plant cultivation on spacecraft or planetary outposts is a promising and actual perspective both for food bioactive molecules production. To this aim, plants response to ionizing radiations, as an important component of space radiation, must be assessed through on-ground experiments due the potentially fatal effects living systems. Hereby, we investigated X-rays -rays exposure tomato ‘hairy root’ cultures (HRCs), which represent solid platform production pharmaceutically relevant molecules,...
Plants respond to ultraviolet stress inducing a self-defence through the regulation of specific gene family members. The UV acclimation is result biochemical and physiological processes, such as enhancement antioxidant enzymatic system accumulation UV-absorbing phenolic compounds (e.g. flavonoids). Globe artichoke an attractive species for studying protein network involved in response, being characterized by remarkable levels inducible antioxidants. Proteomic tools can assist evaluation...