A5041478972- Cervical Cancer and HPV Research
- Virus-based gene therapy research
- Immunotherapy and Immune Responses
- Viral Infections and Vectors
- Extracellular vesicles in disease
- Monoclonal and Polyclonal Antibodies Research
- Polyomavirus and related diseases
- Viral gastroenteritis research and epidemiology
- Vector-Borne Animal Diseases
- Parvovirus B19 Infection Studies
- Endometrial and Cervical Cancer Treatments
- Viral Infections and Outbreaks Research
- Respiratory viral infections research
- MicroRNA in disease regulation
- Plant Virus Research Studies
- Bacteriophages and microbial interactions
- SARS-CoV-2 and COVID-19 Research
- RNA Interference and Gene Delivery
- Hepatitis B Virus Studies
- Animal Virus Infections Studies
- Antenna Design and Analysis
- Transgenic Plants and Applications
- Cancer-related molecular mechanisms research
- Herpesvirus Infections and Treatments
- Protein purification and stability
Istituto Superiore di Sanità
2015-2024
San Gallicano Hospital
2019
Istituti di Ricovero e Cura a Carattere Scientifico
2019
Federico II University Hospital
2008
Ospedale Maggiore
2006
Policlinico Tor Vergata
2006
Istituti Fisioterapici Ospitalieri
2006
Turku University Hospital
2006
University of Turku
2006
Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico
2006
Approximately 12% of human cancers worldwide are associated with infectious agents, which classified by the International Agency for Research on Cancer (IARC) as Group 1 within agents that carcinogenic to humans. Most these viruses. oncogenic viruses include hepatitis C virus, B virus (HBV), T-cell lymphotropic type 1, Epstein-Barr Kaposi sarcoma-associated herpesvirus, immunodeficiency virus-1 and high-risk papillomaviruses (HPVs). In addition, some polyomaviruses suspected inducing cancer...
We recently proved that exosomes engineered in vitro to deliver high amounts of HPV E7 upon fusion with the Nefmut exosome-anchoring protein elicit an efficient anti-E7 cytotoxic T lymphocyte immune response. However, view a potential clinic application this finding, our exosome-based immunization strategy was faced possible technical difficulties including industrial manufacturing, cost production, and storage. To overcome these hurdles, we designed as yet unproven relying on delivery by...
We developed an innovative strategy to induce a cytotoxic T cell (CTL) immune response against protein antigens of choice. It relies on the production exosomes, i.e., nanovesicles spontaneously released by all types. engineered upload huge amounts upon fusion with anchoring (i.e., HIV-1 Nefmut), which is inactive incorporating in exosomes at high levels also when fused foreign proteins. compared immunogenicity uploading human papillomavirus (HPV)-E7 that lentiviral virus-like particles...
We analyzed survivin as a marker of cervical intraepithelial neoplasia (CIN) and high-risk human papillomavirus (HR-HPV) predictor HPV clearance disease outcome in cancer 302 samples (squamous cell carcinomas [SCCs], 150; CIN lesions, 152) by immunohistochemical staining with antibody testing using polymerase chain reaction. HR-HPV types were associated closely SCC. There was significant linear relationship between grade intensity expression (P = .0001). Survivin overexpression also strongly...
Epitheliotropic viruses can find their way into sewage. The aim of the present study was to investigate occurrence, distribution, and genetic diversity Human Papillomaviruses (HPVs) in urban wastewaters. Sewage samples were collected from treatment plants distributed throughout Italy. DNA extracted these analyzed by PCR using five PV-specific sets primers targeting L1 (GP5/GP6, MY09/MY11, FAP59/64, SKF/SKR) E1 regions (PM-A/PM-B), according protocols previously validated for detection...
Severe Acute Respiratory Syndrome (SARS) is a dangerous infection with pandemic potential. It emerged in 2002 and its aetiological agent, the SARS Coronavirus (SARS-CoV), crossed species barrier to infect humans, showing high morbidity mortality rates. No vaccines are currently licensed for SARS-CoV important efforts have been performed during first outbreak develop diagnostic tools. Here we demonstrate transient expression Nicotiana benthamiana of two antigenic determinants SARS-CoV,...
The human papillomavirus 16 (HPV16) E7 oncoprotein can be considered a ‘tumor-specific antigen’ and, therefore, it represents promising target for therapeutic vaccine against HPV-associated tumors. Efficient production of protein with plant-based transient expression system has been already described and was demonstrated that E7-containing crude plant extracts confer partial protection tumor challenge in mouse model system. Before adopting the as cost-effective method an E7-based anti-cancer...
Human papillomaviruses (HPVs) have been detected in urban wastewaters, demonstrating that epitheliotropic viruses can find their way into sewage through the washing of skin and mucous membranes. Papillomavirus shedding faeces is still an unexplored issue. The objective present study was to investigate presence HPVs stool samples. We analysed 103 faecal specimens collected from hospitalized patients with diarrhoea using validated primers able detect α, β γ HPVs. PCR products underwent...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has killed more than 37,000 people in Italy and caused widespread socioeconomic disruption. Urgent measures are needed to contain control the virus, particularly diagnostic kits for detection surveillance, therapeutics reduce mortality among severely affected, vaccines protect remaining population. Here we discuss potential role of plant molecular farming rapid scalable supply protein antigens as reagents vaccine candidates,...
Increased angiogenesis leads to invasion in cervical cancer. Vascular endothelial growth factors (VEGFs) are involved angiogenesis, but molecular links the most important aetiological agent, human papillomavirus (HPV), need clarifying.
Human papillomavirus (HPV)-associated tumors still represent an urgent problem of public health in spite the efficacy prophylactic HPV vaccines. Specific antibodies single-chain format expressed as intracellular (intrabodies) are valid tools to counteract activity target proteins. We previously showed that M2SD intrabody, specific for E7 oncoprotein HPV16 and endoplasmic reticulum HPV16-positive SiHa cells, was able inhibit cell proliferation. Here, we by confocal microscopy colocalize...
To the Editor: Toscana virus (TOSV) is an arthropod-borne RNA (family Bunyaviridae and genus Phlebovirus) transmitted by sandflies in Mediterranean countries. TOSV causes acute meningitis meningoencephalitis patients. In France, cases of infections involving resident populations imported tourists traveling TOSV-endemic countries have been reported (1,2); has also isolated from local wild-caught (1). The fact that human blood on several occasions (2) suggests a potential risk exists for...
Viral outbreaks associated with swimming pools have been described worldwide. The objective of this study was to examine the extent viral contamination in indoor and outdoor pools.Pools were examined for presence human enteric viruses (adenovirus, norovirus enterovirus) nonenteric (papillomavirus polyomavirus-BK, JC, KI, WU Merkel cell). Bacteriological parameters also evaluated. analysed pool waters met microbiological quality standards. Enteric not detected. On other hand, papillomaviruses...
// Carla Amici 1 , Michela Visintin 2 Francesca Verachi 1,3 Paolini 4 Zulema Percario 5 Paola Di Bonito 3 Angela Mandarino Elisabetta Affabris Aldo Venuti and Luisa Accardi University of Rome Tor Vergata, Department Biology, Via della Ricerca Scientifica, Rome, Italy Rottapharm Biotech Srl, Biotherapeutics Discovery, Area Science Park – Basovizza, Trieste, Istituto Superiore di Sanità, Infectious, Parasitic Immunomediated Diseases, Viale Regina Elena, Elena National Cancer Institute,...