A5015312368- Cervical Cancer and HPV Research
- Virus-based gene therapy research
- Immunotherapy and Immune Responses
- Viral Infections and Vectors
- Monoclonal and Polyclonal Antibodies Research
- Endometrial and Cervical Cancer Treatments
- Extracellular vesicles in disease
- Vector-Borne Animal Diseases
- MicroRNA in disease regulation
- Hepatitis B Virus Studies
- Parvovirus B19 Infection Studies
- Viral gastroenteritis research and epidemiology
- Viral Infections and Outbreaks Research
- Cancer-related molecular mechanisms research
- Mosquito-borne diseases and control
- Ovarian cancer diagnosis and treatment
- Polyomavirus and related diseases
- Toxin Mechanisms and Immunotoxins
- Protein purification and stability
- Circular RNAs in diseases
- TGF-β signaling in diseases
- Antenna Design and Analysis
- Herpesvirus Infections and Treatments
- Genital Health and Disease
- Colorectal and Anal Carcinomas
Istituto Superiore di Sanità
2013-2023
San Gallicano Hospital
2019
Istituti di Ricovero e Cura a Carattere Scientifico
2019
Ospedale Maggiore
2006
Policlinico Tor Vergata
2006
Istituti Fisioterapici Ospitalieri
2006
Turku University Hospital
2006
University of Turku
2006
Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico
2006
We analyzed survivin as a marker of cervical intraepithelial neoplasia (CIN) and high-risk human papillomavirus (HR-HPV) predictor HPV clearance disease outcome in cancer 302 samples (squamous cell carcinomas [SCCs], 150; CIN lesions, 152) by immunohistochemical staining with antibody testing using polymerase chain reaction. HR-HPV types were associated closely SCC. There was significant linear relationship between grade intensity expression (P = .0001). Survivin overexpression also strongly...
Epitheliotropic viruses can find their way into sewage. The aim of the present study was to investigate occurrence, distribution, and genetic diversity Human Papillomaviruses (HPVs) in urban wastewaters. Sewage samples were collected from treatment plants distributed throughout Italy. DNA extracted these analyzed by PCR using five PV-specific sets primers targeting L1 (GP5/GP6, MY09/MY11, FAP59/64, SKF/SKR) E1 regions (PM-A/PM-B), according protocols previously validated for detection...
Human papillomaviruses (HPVs) have been detected in urban wastewaters, demonstrating that epitheliotropic viruses can find their way into sewage through the washing of skin and mucous membranes. Papillomavirus shedding faeces is still an unexplored issue. The objective present study was to investigate presence HPVs stool samples. We analysed 103 faecal specimens collected from hospitalized patients with diarrhoea using validated primers able detect α, β γ HPVs. PCR products underwent...
Increased angiogenesis leads to invasion in cervical cancer. Vascular endothelial growth factors (VEGFs) are involved angiogenesis, but molecular links the most important aetiological agent, human papillomavirus (HPV), need clarifying.
Human papillomavirus (HPV)-associated tumors still represent an urgent problem of public health in spite the efficacy prophylactic HPV vaccines. Specific antibodies single-chain format expressed as intracellular (intrabodies) are valid tools to counteract activity target proteins. We previously showed that M2SD intrabody, specific for E7 oncoprotein HPV16 and endoplasmic reticulum HPV16-positive SiHa cells, was able inhibit cell proliferation. Here, we by confocal microscopy colocalize...
// Carla Amici 1 , Michela Visintin 2 Francesca Verachi 1,3 Paolini 4 Zulema Percario 5 Paola Di Bonito 3 Angela Mandarino Elisabetta Affabris Aldo Venuti and Luisa Accardi University of Rome Tor Vergata, Department Biology, Via della Ricerca Scientifica, Rome, Italy Rottapharm Biotech Srl, Biotherapeutics Discovery, Area Science Park – Basovizza, Trieste, Istituto Superiore di Sanità, Infectious, Parasitic Immunomediated Diseases, Viale Regina Elena, Elena National Cancer Institute,...
Telomerase activation and telomere maintenance are essential for cell immortalization represent a rate-limiting step in cancer progression. The E6 oncoprotein of high-risk human papillomavirus (HPV) is known to activate telomerase, but its expression CIN lesions prognostic value cervical (CC) still incompletely understood. As part our HPV-PathogenISS study, series 150 CCs 152 were examined using immunohistochemical (IHC) staining hTERT (telomerase reverse transcriptase), tested HPV PCR with...
Purpose: Single-chain variable fragments (scFvs) are one of the smallest antigen-binding units having invaluable advantage to be expressed by a unique short open reading frame (ORF). Despite their reduced size, spontaneous cell entry scFvs remains inefficient, hence precluding possibility target intracellular antigens. Here, we describe an original strategy deliver inside cells through engineered extracellular vesicles (EVs). This approach relies on properties Human Immunodeficiency Virus...
A transcription system for Toscana virus (TOSV) (a member of the family BUNYAVIRIDAE:, genus PHLEBOVIRUS:) was constructed. For in vivo expression, TOSV uses viral N and L proteins an S-like RNA genome containing chloramphenicol acetyltransferase reporter gene antisense orientation flanked by genomic 5'- 3'-terminal S sequences. It found that represent minimal protein requirement active complex. To investigate possibility reassortment between Rift Valley fever (RVFV), activity their...
Human papillomaviruses (HPVs) are the primary etiological agents of cervical cancer and also involved in development other tumours (skin, head neck). Serological survey HPV infections is important to better elucidate their natural history disclose antigen determinants useful for vaccine development. At present, analysis HPV-specific antibodies has not diagnostic value viral infections, new approaches needed correlate antibody response disease outcome. The aim this study develop a novel...
Comprehensive multivariate models were used to disclose whether any of our previously analyzed 13 markers would be independent predictors intermediate end point in cervical carcinogenesis. The expression the following biomarkers, E-cadherin, extracellular signal-regulated kinase 1, 67-kd laminin receptor (LR67), matrix metalloproteinase 2, tissue inhibitor nuclear factor-kappaB, nm23-H1, p16, proliferating cell antigen, survivin, human telomerase reverse transcriptase, topoisomerase 2alpha,...
Amino-functionalized poly(L-lactide) lamellar single crystals as a valuable substrate for delivery of HPV16-E7 tumor antigen in vaccine development Paola Di Bonito,1 Linda Petrone,1 Gabriele Casini,2 Iolanda Francolini,2 Maria Grazia Ammendolia,3 Luisa Accardi,1 Antonella Piozzi,2 Lucio D’Ilario,2 Andrea Martinelli2 1Department Infectious, Parasitic and Immune-mediated Diseases, Italian National Institute Health, 2Department Chemistry, Sapienza University Rome, Italy; 3Department...
Ebola hemorrhagic fever is caused by the filovirus (EBOV), which one of most aggressive infectious agents known worldwide. The EBOV pathogenesis starts with uncontrolled viral replication and subversion both innate adaptive host immune response. multifunctional VP35 protein involved in this process exerting an antagonistic action against early antiviral alpha/beta interferon (IFN-α/β) response, represents a suitable target for development strategies to control infection. Phage display...
Abstract Background The oncogenic activity of the high risk human papillomavirus type 16 (HPV16) is fully dependent on E6 and E7 viral oncoproteins produced during infection. interfere with cellular homeostasis by promoting proliferation, inhibiting apoptosis blocking epithelial differentiation, driving infected cells towards neoplastic progression. causal relationship between expression E6/E7 transformation allows process hindering two oncoproteins. We previously developed characterized...
Nuclear factor-kappaB (NF-kappaB) has a pivotal function in controlling wide variety of gene functions, and shown to be constitutively activated many human cancers. The molecular links NF-kappaB oncogenic papillomavirus (HPV) cervical intraepithelial neoplasia (CIN) lesions its prognostic value cancer (CC) are incompletely understood. As part our HPV-PathogenISS study, series 150 squamous-cell carcinomas (SCCs) 152 CIN were examined using immunohistochemical staining for NF-kappaB, tested...