A5033439027- Bone Tissue Engineering Materials
- Electrospun Nanofibers in Biomedical Applications
- Dental materials and restorations
- Dental Implant Techniques and Outcomes
- Mesenchymal stem cell research
- Periodontal Regeneration and Treatments
- Tissue Engineering and Regenerative Medicine
- Adenosine and Purinergic Signaling
- Ion Channels and Receptors
- Wound Healing and Treatments
- Angiogenesis and VEGF in Cancer
- Orthopaedic implants and arthroplasty
- Nitric Oxide and Endothelin Effects
- Neurobiology and Insect Physiology Research
- Silk-based biomaterials and applications
- 3D Printing in Biomedical Research
- Congenital heart defects research
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Endodontics and Root Canal Treatments
- Osteoarthritis Treatment and Mechanisms
- Nanocomposite Films for Food Packaging
- Anodic Oxide Films and Nanostructures
- Prostate Cancer Treatment and Research
- Graphene and Nanomaterials Applications
- Nanoplatforms for cancer theranostics
University of Turin
2015-2024
Accademia Albertina delle Belle Arti
2015-2024
During wound healing, biologically active molecules are released from platelets. The rationale of using platelet-rich plasma (PRP) relies on the concentration bioactive and subsequent delivery to healing sites. These have been seldom simultaneously quantified within same PRP preparation. In present study, flexible Bio-Plex system was employed assess a large range cytokines, chemokines, growth factors in 16 healthy volunteers so as determine whether significant baseline differences may be...
Bone is the second most manipulated tissue after blood. Adipose-derived stem cells (ASCs) may become a convenient source of MSC for bone regenerative protocols. Surprisingly, little known about significant biomolecules these produce and release being osteoinduced. Therefore, present study aimed at dosing 13 candidates chosen among representative cytokines, chemokines, growth factors within conditioned media osteodifferentiated undifferentiated ASCs. Two acknowledged osteoblastic cell models,...
The aim of this in vitro study was to evaluate the early cell response and protein adsorption elicited by argon plasma treatment different commercially available titanium surfaces via a chair-side device. Sterile disks made grade 4 ( n = 450, 4-mm diameter) with 3 surface topographies (machined, sprayed, zirconia blasted acid etched) were allocated receive testing treatments (2% 10% adhesion MC3T3-E1 MG-63). Furthermore, specimens divided undergo 1) (10 W, 1 bar for 12 min) reactor, 2)...
Heme is required for cell respiration and survival. Nevertheless, its intracellular levels need to be finely regulated avoid heme excess, which may catalyze the production of reactive oxygen species (ROS) promote death. Here, we show that alteration homeostasis in endothelial cells due loss exporter FLVCR1a, results impaired angiogenesis. In vitro, FLVCR1a silencing causes defective tubulogenesis poor viability accumulation. Consistently, endothelial-specific Flvcr1a knockout mice aberrant...
Endothelial cell adhesion and migration are critical steps of the angiogenic process, whose dysfunction is associated with tumor growth metastasis. The TRPM8 channel has recently been proposed to play a protective role in prostate cancer by impairing motility. However, mechanisms which it could influence vascular behavior unknown. Here, we reveal novel non-channel function for that unexpectedly acts as Rap1 GTPase inhibitor, thereby inhibiting endothelial motility, independently pore...
Purinergic signaling is involved in inflammation and cancer. Extracellular ATP accumulates tumor interstitium, reaching hundreds micromolar concentrations, but its functional role on vasculature endothelium unknown. Here we show that high doses (>20 μM) strongly inhibit migration of endothelial cells from human breast carcinoma (BTEC), not normal microvascular EC. Lower (1-10 μM result ineffective. The anti-migratory activity associated with cytoskeleton remodeling significantly prevented by...
Ceramic materials are widely used for biomedical applications because of their remarkable biological and mechanical properties. Composites made alumina zirconia particularly interesting owing to higher toughness with respect the monolithic materials. On this basis, present study is focused on in vivo behavior toughened (ATZ) dental implants treated a hydrothermal process. A minipig model was implemented assess bone healing through histology mRNA expression at different time points (8, 14,...
This in vitro study tested the effects of argon atmospheric pressure dielectric barrier discharge (APDBD) on different implant surfaces with regard to physical changes, bacterial decontamination, and osteoblast adhesion.Seven hundred twenty disks three surface topographies-machined (MAC), titanium plasma-sprayed (TPS), zirconia-blasted acid-etched (ZRT)-were this experiment. Bacterial adhesion tests were performed repeatedly a simplified biofilm Streptococcus mitis. Bacteria incubated...
Implantable devices need specific tailored surface morphologies and chemistries to interact with the living systems or actively induce a biological response also by release of drugs proteins. These customized requirements foster technologies that can be implemented in additive manufacturing systems. Here, we present novel approach based on spraying processes allow control separately topographic features submicron range (∼60 nm 2 μm), ammine carboxylic chemistry, fluorophore even...
Background: Transient receptor potential (TRP) channels control multiple processes involved in cancer progression by modulating cell proliferation, survival, invasion and intravasation, as well as, endothelial (EC) biology tumor angiogenesis. Nonetheless, a complete TRP expression signature vessels, including prostate (PCa), is still lacking. Methods: In the present study, we profiled qPCR of all human tumor-derived ECs (TECs) comparison with TECs from breast renal tumors. We further...
The synergistic crosstalk between osteodifferentiating stem cells and endothelial (ECs) gained the deserved consideration, shedding light on role of angiogenesis for bone formation healing. A deep understanding molecular basis underlying mutual influence mesenchymal (MSCs) ECs in osteogenic process may help improve greatly regeneration. Here, authors demonstrated that MSCs co-cultured with promote recruitment. Moreover, through use 3D co-culture systems, we showed are turn able to further...
Abstract The Feline Leukemia Virus Subgroup C Receptor 1a (FLVCR1a) is a transmembrane heme exporter essential for embryonic vascular development. However, the exact role of FLVCR1a during blood vessel development remains largely undefined. Here, we show that highly expressed in angiogenic endothelial cells (ECs) compared to quiescent ECs. Consistently, ECs lacking give rise structurally and functionally abnormal networks multiple models developmental pathologic angiogenesis. Firstly,...
Adipose tissue-derived stem cells (ASCs) are a promising tool for the treatment of bone diseases or skeletal lesions, thanks to their ability potentially repair damaged tissue. One major limitations ASCs is represented by necessity be isolated and expanded through in vitro culture; thus, strong interest was generated adipose stromal vascular fraction (SVF), noncultured ASCs. SVF heterogeneous cell population, directly obtained after collagenase In order investigate compare bone-regenerative...
Hypercholesterolemia is one of the major causes cardiovascular disease, risk which further increased if other forms dyslipidemia occur. Current therapeutic strategies include changes in lifestyle coupled with drug administration. Statins represent most common approach, but they may be insufficient due to onset resistance mechanisms and side effects. Consequently, patients mild hypercholesterolemia prefer use food supplements since these are perceived safer. Here, we investigate phytochemical...
Great efforts have been made to improve bone regeneration techniques owing a growing variety of sources stem cells suitable for autologous transplants. Specifically, adipose-derived (ASCs) and stems from human exfoliated deciduous teeth (SHED) hold great potential tissue engineering cell therapy. After preliminary characterization the main biomolecules ASCs SHED released in their conditioned media, were kept both normal osteo-inducing conditions. Conventional assays performed prove...
Apical periodontitis includes periapical granulomas and radicular cysts, which are histologically distinguished by the absence presence of an epithelial lining, respectively. The main cause apical is bacterial colonization root canal space. This research aimed at assessing whether how cysts differ in terms microbiota using high throughput amplicon target sequencing (HTS) techniques. study included 5 cases Periapical Granulomas (PGs) Radicular Cysts (RCs) selected on base histology out 37...
It has long been known that the conditionally essential polyunsaturated arachidonic acid (AA) regulates cerebral blood flow (CBF) through its metabolites prostaglandin E2 and epoxyeicosatrienoic acid, which act on vascular smooth muscle cells pericytes to vasorelax microvessels. However, AA may also elicit endothelial nitric oxide (NO) release an increase in intracellular Ca2+ concentration ([Ca2+]i). Herein, we adopted NO imaging, combined with immunoblotting, assess whether induces signals...
This study was conducted to compare the effects of an innovative plasma surface treatment device that does not need a gas supply for titanium disks with two different topographies: prototypical machined (MAC) and one most diffused roughened ones (SL) obtained through grit blasting acid etching. A total 200-MAC 200-SL were used. Each group divided into four sub-groups 40 samples each subjected five tests. Among these, 150-MAC 150-SL considered test group, they treated 15, 30, 60 s after being...
Abstract We recently showed that arachidonic acid (AA) triggers calcium signals in endothelial cells derived from human breast carcinoma (B-TEC). In particular, AA-dependent Ca2+ entry is involved the early steps of tumor angiogenesis vitro. Here, we investigated multiple roles nitric oxide (NO) and cyclic AMP/protein kinase A (PKA) pathways AA-mediated signaling same cells. B-TEC stimulation with 5 μmol/L AA resulted NO synthase (NOS) phosphorylation at Ser1177, release was measured...