A5010445895- Cardiac Ischemia and Reperfusion
- Nitric Oxide and Endothelin Effects
- Cardiac Arrest and Resuscitation
- Sulfur Compounds in Biology
- Anesthesia and Neurotoxicity Research
- Eicosanoids and Hypertension Pharmacology
- Mitochondrial Function and Pathology
- Neuroscience of respiration and sleep
- Adenosine and Purinergic Signaling
- Biochemical effects in animals
- Systemic Lupus Erythematosus Research
- Adipose Tissue and Metabolism
- Mechanical Circulatory Support Devices
- Mesenchymal stem cell research
- Cardiovascular Function and Risk Factors
- Apelin-related biomedical research
- Cardiovascular and exercise physiology
- Tissue Engineering and Regenerative Medicine
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cardiac Valve Diseases and Treatments
- Organ Transplantation Techniques and Outcomes
- Heme Oxygenase-1 and Carbon Monoxide
- Electron Spin Resonance Studies
- Pesticide and Herbicide Environmental Studies
- Angiogenesis and VEGF in Cancer
Ospedale San Luigi Gonzaga
2012-2025
University of Turin
2014-2024
Universidade Federal do Rio Grande do Sul
2017
Lakehead University
2014
Istituto Nazionale per le Ricerche Cardiovascolari
2009
National Cancer Institute
2004-2006
National Institutes of Health
2003-2006
University of Amsterdam
2006
Johns Hopkins University
2005
University of California, Los Angeles
2004-2005
NO produced in tumors can either positively or negatively regulate growth. To examine this dichotomy, effects of concentration and duration on the posttranslational regulation several key proteins were examined human breast MCF7 cells under aerobic conditions. We found that different thresholds appear to elicit a discrete set signal transduction pathways. At low steady-state concentrations (<50 nM), extracellular signal-regulated kinase (ERK) phosphorylation was induced via guanylate...
Postconditioning (PostC) maneuvers allow post-ischemic accumulation of autacoids, which trigger protection. We tested if PostC-triggering includes bradykinin (BK) B2 receptor activation and its downstream pathway.Isolated rat hearts underwent 30 min ischemia 120 reperfusion. Infarct size was evaluated using nitro-blue tetrazolium staining. In Control infarct 61+/-5% risk area. PostC (5 cycles 10 s reperfusion/ischemia) reduced to 22+/-4% (p<0.01). protection abolished by BK...
Diazeniumdiolates, more commonly referred to as NONOates, have been extremely useful in the investigation of biological effects nitric oxide (NO) and related nitrogen oxides. The NONOate Angeli's salt (Na2N2O3) releases nitroxyl (HNO) under physiological conditions exhibits unique cardiovascular features (i.e., positive inotropy/lusitropy) that may relevance for pharmacological treatment heart failure. In search new, organic-based compounds release HNO, we examined isopropylamine (IPA/NO;...
Purinergic signaling is involved in inflammation and cancer. Extracellular ATP accumulates tumor interstitium, reaching hundreds micromolar concentrations, but its functional role on vasculature endothelium unknown. Here we show that high doses (>20 μM) strongly inhibit migration of endothelial cells from human breast carcinoma (BTEC), not normal microvascular EC. Lower (1-10 μM result ineffective. The anti-migratory activity associated with cytoskeleton remodeling significantly prevented by...
The recent determination that Angeli's salt may have clinical application as a nitrogen oxide donor for treatment of cardiovascular diseases such heart failure has led to renewed interest in the mechanism and products thermal decomposition under physiological conditions. In this report, several mechanisms are evaluated experimentally by quantum mechanical calculations determine whether HNO is fact released from neutral, aerobic solution. product autoxidation also considered.
Background: Glyphosate has been extensively used as herbicide since the early 1970s. The daily exposure limit is set at 0.3 mg/kg bw/d in Europe and 1.75 USA. Among its derivatives, aminomethylphosphonic acid most stable abundant. Understanding their biological effects then requires reliable methods for quantification samples. Methods: We developed validated a fast, low-cost, chromatographic method determining glyphosate concentrations. validation included following parameters: specificity,...