A5055810398- Mitochondrial Function and Pathology
- Retinal Development and Disorders
- Retinal Diseases and Treatments
- ATP Synthase and ATPases Research
- Photoreceptor and optogenetics research
- Retinopathy of Prematurity Studies
- Metabolism and Genetic Disorders
- Venomous Animal Envenomation and Studies
- Extracellular vesicles in disease
- Glaucoma and retinal disorders
- Advanced Fluorescence Microscopy Techniques
- Endoplasmic Reticulum Stress and Disease
- Adenosine and Purinergic Signaling
- Electromagnetic Fields and Biological Effects
- Photosynthetic Processes and Mechanisms
- Microbial Inactivation Methods
- Ion channel regulation and function
- Neonatal Respiratory Health Research
- Genetics, Aging, and Longevity in Model Organisms
- Biochemical and Structural Characterization
- Planarian Biology and Electrostimulation
- Biochemical effects in animals
- Muscle metabolism and nutrition
- Medical and Biological Ozone Research
- Marine Invertebrate Physiology and Ecology
University of Genoa
2014-2023
Exosomes are secreted nanovesicles that able to transfer RNA and proteins target cells. The emerging role of mesenchymal stem cell (MSC) exosomes as promoters aerobic ATP synthesis restoration in damaged cells, prompted us assess whether they contain an extramitochondrial respiration capacity. were isolated from culture medium human MSCs umbilical cord ≥37-wk-old newborns or between 28- 30-wk-old (i.e., term preterm infants). Characterization samples was conducted by cytofluorometry....
Oxidative phosphorylation (OXPHOS) is not only the main source of ATP for cell, but also a major reactive oxygen species (ROS), which lead to oxidative stress. At present, mitochondria are considered organelles responsible OXPHOS, in last years we have demonstrated that it can occur outside mitochondrion. Myelin sheath able conduct an aerobic metabolism, producing hypothesized transferred axon, support its energetic demand. In this work, spectrophotometric, cytofluorimetric, and luminometric...
Potent neuroprotective effects of photobiomodulation with 670 nm red light (RL) have been demonstrated in several models retinal disease. RL improves mitochondrial metabolism, reduces inflammation and oxidative cell stress, showing its ability to enhance visual function. However, the current knowledge is limited main hypothesis that respiratory chain complex IV, cytochrome c oxidase, serves as primary target RL. Here, we demonstrate a comprehensive cellular, molecular, functional...
The initial events of vision at low light take place in vertebrate retinal rods. rod outer segment consists a stack flattened disks surrounded by the plasma membrane. A list proteins that reside has not been achieved yet. We present first comprehensive proteomic analysis purified disks, obtained combining results two-dimensional gel electrophoresis separation disk to MALDI-TOF or nLC-ESI-MS/MS mass spectrometry techniques. Intact were isolated from bovine segments method minimizes...
The rod outer segments (OS) of the retina are specialized organelles where phototransduction takes place. mitochondrial electron transport complexes I-IV, cytochrome c and Fo F1 -ATP synthase functionally expressed in OS disks. Here, we have studied effect some polyphenolic compounds acting as inhibitors ATPase/synthase activity on ectopic - ATP synthase. mechanism apoptosis was also investigated studying expression c, caspase 9 3 Apaf-1.We prepared from fresh bovine retinae....
The basic processes harvesting chemical energy for life are driven by proton (H(+)) movements. These accomplished the mitochondrial redox complex V, integral membrane supramolecular aggregates, whose structure has recently been described advanced studies. did not identify classical aqueous pores. It was proposed that H(+) transfer oxidative phosphorylation (OXPHOS) does occur between sources and sinks, where an barrier would be insurmountable. This suggests a novel hypothesis transfer. A...
Purpose Microvesicles (MVs), 200–1000 nm bodies budding from the cell plasma membrane, are a promising source of biomarkers. This study aimed at comparing proteome MVs collected by ultracentrifugation cultured Mesenchymal Stem Cells (MSCs) Human Umbilical Cord Preterm newborns (<34‐weeks gestational age) in comparison to infants Term (≥37 weeks). discovery was designed establish signature prematurity. Experimental design Orbitrap MS, statistical, bioinformatics and biochemical analyses...
Background Information The rod outer segment (OS) is the specialised organelle where phototransduction takes place. Our previous proteomic and biochemical analyses on purified disks showed functional expression of respiratory chain complexes I–IV F 1 o ‐ATP synthase in OS disks, as well active soluble tricarboxylic acid cycle enzymes. Here, we focussed our study whole that contains cytosol plasma membrane native flattened saccules, unlike spherical osmotically intact disks. Results were from...
Several studies have shown that mammalian retinal rod outer segments (OS) are peculiar structures devoid of mitochondria, characterized by ectopic expression the molecular machinery for oxidative phosphorylation. Such aerobic metabolism would provide chemical energy phototransduction taking place in OS. Natural polyphenols include a large variety molecules having pleiotropic effects, ranging from anti-inflammatory to antioxidant and others. Our goal present study was investigate potential...
Abstract The effects of extremely low frequency magnetic fields (ELF‐MF) on acetylcholinesterase (AChE) activity synaptosomal membranes were investigated. Sinusoidal with 50 Hz and different amplitudes caused AChE to decrease about 27% a threshold 0.74 mT. in enzymatic was independent the time permanence field completely reversible. Identical results obtained exposure static MF same amplitudes. Moreover, inhibitory are spread over windows maximal values at 60, 200, 350, 475 Hz. When...
A surface extract of the aerial parts Salvia tingitana afforded a nor-sesterterpenoid (1) and eight new sesterterpenoids (2–̵9), along with five known sesterterpenoids, labdane one abietane diterpenoid, sesquiterpenoid, four flavonoids. The structures compounds were established by 1D 2D NMR spectroscopy, HRESIMS, VCD data Mosher's esters analysis. antimicrobial activity was evaluated against 30 human pathogens including 27 clinical strains three isolates marine origin for their possible...
Purpose The retinal rod outer segment (OS) disk membranes, devoid of mitochondria, conducts oxidative phosphorylation (OxPhos). This study aimed at identifying which proteins expressed in the OS disks determined considerable adenosine-5'-triphosphate production and oxygen consumption observed comparison with mitochondria. Procedures Characterization was conducted by immunogold transmission electron microscopy on sections. OxPhos studied oximetry luminometry. proteomes mitochondria purified...
The vertebrate retina is an array of "narrow-capture" photoreceptive elements diverse cellular types that allow the fine spatial resolution characteristic vision. Imaging photoreceptors and whole has been previously reported; however, both were achieved exclusively after fixation. We report our development a new technique for imaging live bovine retinas ex vivo. Using this technique, we conducted fluorescence confocal laser scanning microscopic retinas. Eyecups incubated with conventional...