A5050736407- Advanced Fluorescence Microscopy Techniques
- Advanced Electron Microscopy Techniques and Applications
- Cell Image Analysis Techniques
- Genomics and Chromatin Dynamics
- Photodynamic Therapy Research Studies
- X-ray Diffraction in Crystallography
- DNA and Nucleic Acid Chemistry
- Nanoplatforms for cancer theranostics
- Retinal Development and Disorders
- Crystallization and Solubility Studies
- Nuclear Structure and Function
- Image Processing Techniques and Applications
- ATP Synthase and ATPases Research
- Near-Field Optical Microscopy
- Digital Holography and Microscopy
- Optical Polarization and Ellipsometry
- Advanced X-ray Imaging Techniques
- Mitochondrial Function and Pathology
- Photoacoustic and Ultrasonic Imaging
- Advanced biosensing and bioanalysis techniques
- Nanoparticles: synthesis and applications
- Retinopathy of Prematurity Studies
- Redox biology and oxidative stress
- Hemoglobin structure and function
- Blood properties and coagulation
Italian Institute of Technology
2015-2023
University of Genoa
2013-2017
Abstract Imaging of nuclear structures within intact eukaryotic nuclei is imperative to understand the effect chromatin folding on genome function. Recent developments super-resolution fluorescence microscopy techniques combine high specificity, sensitivity, and less-invasive sample preparation procedures with sub-diffraction spatial resolution required image at nanoscale. Here, we present a method enhance stimulated-emission depletion (STED) microscope based only modulation STED intensity...
The interaction between RAD51 and BRCA2 plays a key role in homologous recombination (HR), critical DNA repair mechanism essential for the survival of cancer cells. Disrupting this increases sensitivity cells to chemotherapeutic agents. Here, we employed silico methods design novel class aptamers-customized single-stranded oligonucleotides-specifically engineered bind RAD51. These aptamers were developed with aim selectively modulating RAD51's nuclear recruitment its processes. leading...
Carbohydrate-conjugated silicon(IV) phthalocyanines with bimodal photoactivity were developed as probes both fluorescent labeling and photosensitizing capabilities, the concomitant photoinduced inactivation of Gram-positive Gram-negative models was explored. The maltohexaose-conjugated photoprobe provides a dual readout to distinguish between groups pathogens, only species inactivated, even though appeared labeled near-infrared luminescence. Antibiotic resistance did not hinder phototoxic...
The rod outer segments (OS) of the retina are specialized organelles where phototransduction takes place. mitochondrial electron transport complexes I-IV, cytochrome c and Fo F1 -ATP synthase functionally expressed in OS disks. Here, we have studied effect some polyphenolic compounds acting as inhibitors ATPase/synthase activity on ectopic - ATP synthase. mechanism apoptosis was also investigated studying expression c, caspase 9 3 Apaf-1.We prepared from fresh bovine retinae....
Extrusion of apoptotic cells from epithelial tissues requires orchestrated morphological rearrangements the cell and its neighbors. However, connections between cascade events leading to extrusion are not fully understood. Here, we characterize an apical actin ring (EAAR) that is assembled within drives extrusion. Caspase-mediated cleavage myotonic dystrophy kinase–related CDC42-binding kinase-α (MRCKα) triggers a signaling pathway leads assembly EAAR pulls bundles, resulting in compaction...
Background Information The rod outer segment (OS) is the specialised organelle where phototransduction takes place. Our previous proteomic and biochemical analyses on purified disks showed functional expression of respiratory chain complexes I–IV F 1 o ‐ATP synthase in OS disks, as well active soluble tricarboxylic acid cycle enzymes. Here, we focussed our study whole that contains cytosol plasma membrane native flattened saccules, unlike spherical osmotically intact disks. Results were from...
Circular dichroism imaging has proved a powerful and simple method for extracting information on chiral molecules without specific fluorescent labels. Numerous mathematical models show that outside the absorption band, circular signal comes from scattering interaction brings additional about organization of biopolymers. With this article, we propose fast to control polarization states moving parts, by means photoelastic modulator. We implemented technique modified commercial confocal...
Bioavailability of photosensitizers for cancer photodynamic therapy is often hampered by their low solubility in water. Here, we overcome this issue using the water-soluble protein apomyoglobin (apoMb) as a carrier photosensitizer hypericin (Hyp). The Hyp-apoMb complex quickly uptaken HeLa and PC3 cells at submicromolar concentrations. Fluorescence emission exploited to localize cellular distribution photosensitizer. plasma membrane rapidly efficiently loaded, fluorescence observed cytoplasm...
Deciphering the spatiotemporal coordination between nuclear functions is important to understand its role in maintenance of human genome. In this context, super-resolution microscopy has gained considerable interest because it can be used probe spatial organization functional sites intact single-cell nuclei 20-250 nm range. Among methods that quantify colocalization from multicolor images, image cross-correlation spectroscopy (ICCS) offers several advantages, namely does not require a...
Abstract Mutations in PRoline Rich Transmembrane protein 2 (PRRT2) cause pleiotropic syndromes including benign infantile epilepsy, paroxysmal kinesigenic dyskinesia, episodic ataxia, that share the character of clinical manifestations. PRRT2 is a neuronal plays multiple roles regulation development, excitability, and neurotransmitter release. To better understand physiopathology these phenotypes, we investigated interactome mouse brain by pulldown-based proteomic approach identified α1 α3...
Dynamic biological systems present challenges to existing three-dimensional (3D) optical microscopes because of their continuous temporal and spatial changes. Most techniques are rigid in adapting the acquisition parameters over time, as confocal microscopy, where a laser beam is sequentially scanned at predefined sampling rate pixel dwell time. Such lack tunability forces user provide scan parameters, which may not be optimal, based on best assumption before an starts. Here, we developed...
The rod Outer Segment (OS) disc, an organelle devoid of mitochondria, is specialized in phototransduction, a process requiring continual chemical energy supply. We have shown that OS discs express functional mitochondrial electron transport chains, F o 1 ‐ATP synthase and the tricarboxylic acid cycle enzymes, all features. Here, we focus on oxygen consumption adenosine triphosphate (ATP) synthesis by analysing chain I‐III‐IV II‐II‐IV pathways, supported reduced nicotinamide adenine...
Epigenetic dysregulation of chromatin is one the hallmarks cancer development and progression, it continuously investigated as a potential general bio-marker this complex disease. One nuclear factors involved in gene regulation unique DEK protein-a histone chaperon modulating topology. expression levels increase significantly from normal to cells, hence raising possibility using tumor marker. Although known be implicated epigenetic transcriptional regulation, details these interactions their...
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Assessing the mechanical behavior of nano- and micron-scale particles with complex shapes is fundamental in drug delivery. Although different techniques are available to quantify bulk stiffness static conditions, there still uncertainty assessing particle deformability dynamic conditions. Here, a microfluidic chip designed, engineered, validated as platform assess fluid-borne particles. Specifically, potassium hydroxide (KOH) wet etching was used realize channel incorporating series...
Abstract Dynamic biological systems present challenges to existing three-dimensional (3D) optical microscopes because of their continuous temporal and spatial changes. Most techniques are based on rigid architectures, as in confocal microscopy, where a laser beam is sequentially scanned at predefined sampling rate pixel dwell time. Here, we developed volumetric Lissajous microscopy achieve unsurpassed 3D scanning speed with tunable rate. The system combines an acoustic liquid lens for axial...