A5008535698- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Alzheimer's disease research and treatments
- RNA regulation and disease
- SARS-CoV-2 and COVID-19 Research
- RNA modifications and cancer
- Amyotrophic Lateral Sclerosis Research
- Animal Virus Infections Studies
- interferon and immune responses
- Calcium signaling and nucleotide metabolism
- Advanced Fluorescence Microscopy Techniques
- vaccines and immunoinformatics approaches
- Cellular transport and secretion
- DNA and Nucleic Acid Chemistry
- Lipid Membrane Structure and Behavior
- Heat shock proteins research
- Neuroinflammation and Neurodegeneration Mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Parkinson's Disease Mechanisms and Treatments
- Advanced biosensing and bioanalysis techniques
- Pancreatic function and diabetes
- Conducting polymers and applications
- Genetics, Aging, and Longevity in Model Organisms
German Center for Neurodegenerative Diseases
2025
Italian Institute of Technology
2020-2024
Sapienza University of Rome
2020-2024
UK Dementia Research Institute
2021
King's College London
2021
Prediction of protein-RNA interactions is important to understand post-transcriptional events taking place in the cell. Here we introduce catRAPID omics v2.0, an update our web server dedicated computation interaction propensities at transcriptome- and RNA-binding proteome-level 8 model organisms. The accepts multiple input protein or RNA sequences computes their scores on updated precompiled libraries. Additionally, it now possible predict between a custom set set. Considerable effort has...
Abstract Specific elements of viral genomes regulate interactions within host cells. Here, we calculated the secondary structure content >2000 coronaviruses and computed >100 000 human protein with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The genomic regions display different degrees conservation. SARS-CoV-2 domain encompassing nucleotides 22 500–23 is conserved both at sequence structural level. upstream downstream, however, vary significantly. This part...
Biomolecular condensates serve as membrane-less compartments within cells, concentrating proteins and nucleic acids to facilitate precise spatial temporal orchestration of various biological processes. The diversity these processes the substantial variability in condensate characteristics present a formidable challenge for quantifying their molecular dynamics, surpassing capabilities conventional microscopy. Here, we show that our single-photon microscope provides comprehensive live-cell...
The development of methods able to modulate the binding affinity between proteins and peptides is paramount biotechnological interest in view a vast range applications that imply designed polypeptides capable impair or favour Protein-Protein Interactions. Here, we applied peptide design algorithm based on shape complementarity optimization electrostatic compatibility provided first experimental vitro proof efficacy algorithm. Focusing interaction SARS-CoV-2 Spike Receptor-Binding Domain...
The interaction between RAD51 and BRCA2 plays a key role in homologous recombination (HR), critical DNA repair mechanism essential for the survival of cancer cells. Disrupting this increases sensitivity cells to chemotherapeutic agents. Here, we employed silico methods design novel class aptamers-customized single-stranded oligonucleotides-specifically engineered bind RAD51. These aptamers were developed with aim selectively modulating RAD51's nuclear recruitment its processes. leading...
Nucleic acids can act as potent modulators of protein aggregation, and RNA has the ability to either hinder or facilitate assembly, depending on molecular context. In this study, we utilized a computational approach characterize physico-chemical properties regions involved in amyloid aggregation. various experimental datasets, observed that while core is hydrophobic highly ordered, external regions, which are more disordered, display distinct tendency interact with nucleic acids. To validate...
ABSTRACT Specific elements of viral genomes regulate interactions within host cells. Here, we calculated the secondary structure content >2000 coronaviruses and computed >100000 human protein with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The genomic regions display different degrees conservation. SARS-CoV-2 domain encompassing nucleotides 22500 – 23000 is conserved both at sequence structural level. upstream downstream, however, vary significantly. This part codes...
Frontotemporal dementia and amyotrophic lateral sclerosis are fatal incurable neurodegenerative diseases linked to the pathological aggregation of TDP-43 protein. This is an essential DNA/RNA-binding protein involved in transcription regulation, pre-RNA processing, RNA transport. Having suitable animal models study mechanisms crucial develop treatments against disease. We have previously demonstrated that killifish Nothobranchius furzeri offers advantage being shortest-lived vertebrate with...
ABSTRACT Nucleic acids can act as potent modulators of protein aggregation, and RNA is able to either hinder or facilitate assembly depending on the molecular context. Here we used a computational approach characterize physico-chemical properties regions involved in amyloid aggregation. In different experimental datasets observed that, while core hydrophobic highly ordered, external regions, more disordered, display distinct tendency interact with nucleic acids. To validate our predictions,...
The accumulation of alpha-synuclein (aS) protein aggregates in cells is a typical hallmark several neurodegenerative diseases. Recent studies have shown that the aberrant transition monomeric aS into solid-like can occur via an intermediate liquid-like state, where partitions between dense and dilute phases. Although not typically recognized as RNA-binding protein, it bind RNA under aggregation conditions. However, impact on dynamics within phases remains unclear. Here, we employ combination...
Abstract Quantitative fluorescence microscopy is experiencing an important revolution thanks to single-photon array detectors. These detectors provide users with so far inaccessible specimen information: The distribution of the specimen’s emission at level and high spatiotemporal sampling. In laser-scanning microscopy, this photon-resolved measurement has enabled robust lifetime imaging sub-diffraction spatial resolution, thus opening new perspectives for structural functional imaging....
Abstract Frontotemporal dementia and amyotrophic lateral sclerosis are fatal incurable neurodegenerative diseases linked to the pathological aggregation of TDP-43 protein. This is an essential DNA/RNA binding protein involved in transcription regulation, pre-RNA processing RNA transport. Having suitable animal models study mechanisms crucial develop treatments against disease. We have previously demonstrated that killifish Nothobranchius furzeri offers unique advantage as a model system it...