A5034295204- Ion Transport and Channel Regulation
- Electrolyte and hormonal disorders
- Ion channel regulation and function
- Birth, Development, and Health
- Renal function and acid-base balance
- Pediatric Urology and Nephrology Studies
- Neuroscience and Neuropharmacology Research
- Receptor Mechanisms and Signaling
- Renal and related cancers
- Neonatal Respiratory Health Research
- Hormonal Regulation and Hypertension
- Chronic Kidney Disease and Diabetes
- Neonatal Health and Biochemistry
- Dialysis and Renal Disease Management
- Pancreatic function and diabetes
- Neurotransmitter Receptor Influence on Behavior
- Biomedical Research and Pathophysiology
- Renin-Angiotensin System Studies
- Neuroendocrine regulation and behavior
- Thermoregulation and physiological responses
- Aldose Reductase and Taurine
- Protein Kinase Regulation and GTPase Signaling
- Diet and metabolism studies
- Lipid Membrane Structure and Behavior
- Renal Diseases and Glomerulopathies
Karolinska Institutet
2014-2024
Science for Life Laboratory
2013-2024
St Göran's Children's Hospital
1991-2015
Milbank Memorial Fund
2014
Svenska Örtmedicinska Institute
2014
Karolinska University Hospital
1969-2013
Sachs' Children and Youth Hospital
2012
University of Oslo
1991-2009
Boston Children's Hospital
1988-2008
Rockefeller University
1991-2006
The plant-derived steroid, digoxin, a specific inhibitor of Na,K-ATPase, has been used for centuries in the treatment heart disease. Recent studies demonstrate presence digoxin analog, ouabain, mammalian tissue, but its biological role not elucidated. Here, we show renal epithelial cells that doses causing only partial Na,K-ATPase inhibition, acts as inducer regular, low-frequency intracellular calcium ([Ca(2+)](i)) oscillations elicit activation transcription factor, NF-kappa B. Partial...
We have examined two distinct protein kinases, cAMP-dependent kinase and C, for their ability to phosphorylate regulate the activity of three different types Na+,K(+)-ATPase preparation. phosphorylated purified shark rectal gland a stoichiometry approximately 1 mol phosphate per alpha subunit. Protein C 2 The phosphorylation by each kinases was associated with an inhibition about 40-50%. These also inhibited partially preparation from rat renal cortex present in preparations basolateral...
ABSTRACT. Aperia, A., Broberger, B., Klinder, G., Herin, P. and Zetterström, R. (Department of Paediatrics, Karolinska Institute, St. Göran's Children's Hospital, Stockholm Huddinge Huddinge, Sweden). Postnatal deveopment renal function in preterm full‐term infants. Acta Paediatr Scand, 70:183, 1981. –This study has been designed to examine the effect gestational age (GA) on postnatal development performed pre‐term (PT) infants (GA=30–34 weeks) (FT) (GA=39–41 weeks). ranged from 1–35 days....
Renal sodium metabolism, a major determinant of blood pressure, is regulated with great precision by variety endocrine, autocrine, and neuronal factors. Although these factors are known to regulate metabolism affecting the rate tubular reabsorption, molecular mechanisms which they act poorly understood. Na+,K(+)-ATPase plays pivotal role for reabsorption in all segments. The activity this enzyme can be dynamically phosphorylation dephosphorylation. Here we summarize both old new evidence...
We studied the effect of dopamine (DA) on Na+-K+-ATPase activity in proximal convoluted tubule (PCT) segments dissected from perfused rat kidneys. DA inhibited a dose-dependent manner. Inhibition was significant with 10(-7) M and maximal 10(-4) DA. The inhibition reversible. Enzyme occurred presence antagonist, metoclopramide, but not when 10(5) DA1 DA2 agonists fenoldopam mesylate LY 171555 were added absence In PCT incubated precursor dopa, also inhibited. However, dopa did inhibit sodium...
Aquaporin-4 (AQP4) plays an important role in the basolateral movement of water collecting duct. Here we show that this channel can be dynamically regulated. Water permeability ( P f ) was measured individual LLC-PK 1 cells were transiently transfected with AQP4. To identify which transfected, AQP4 tagged at NH 2 terminus green fluorescent protein. Transfected showed a strong signal membrane and low-to-negligible cytosol apical membrane. Activation protein kinase C (PKC) phorbol...
Phosphorylation of Ser 256 , in a PKA consensus site, AQP2 (p-AQP2) appears to be critically involved the vasopressin-induced trafficking AQP2. In present study, affinity-purified antibodies that selectively recognize phosphorylated at were developed. These used determine 1) subcellular localization p-AQP2 rat kidney and 2) changes distribution and/or levels response [desamino-Cys 1 ,d-Arg 8 ]vasopressin (DDAVP) treatment or V 2 -receptor blockade. Immunoelectron microscopy revealed was...
Abstract Background The Na + ,K -ATPase plays an important role for ion homeostasis in virtually all mammalian cells, including neurons. Despite this, there is as yet little known about the isoform specific distribution Results With help of superresolving stimulated emission depletion microscopy spatial dendritic spines cultured striatum neurons have been dissected. found compartmentalized provides a strong evidence confinement neuronal (α3 isoform) postsynaptic region spine. Conclusions A...
Abstract Astrocytes play a key role for maintenance of brain water homeostasis, but little is known about mechanisms short‐term regulation astrocyte permeability. Here, we report that glutamate increases permeability and the molecular target this effect aquaporin‐4 (AQP4) serine 111 residue, which in strategic position control channel gating. The involves activation group I metabotropic receptors (mGluR), intracellular calcium release, calcium/calmodulin‐dependent protein kinase II (CaMKII)...
Metabotropic glutamate receptor 5 (mGluR5) is highly expressed in the mammalian central nervous system (CNS). It involved multiple physiological functions and a target for treatment of various CNS disorders, including schizophrenia. We report that Norbin, neuron-specific protein, physically interacts with mGluR5 vivo, increases cell surface localization receptor, positively regulates signaling. Genetic deletion Norbin attenuates mGluR5-dependent stable changes synaptic function measured as...
Vasopressin plays an essential role for the regulation of water balance by activating collecting duct-specific channel, aquaporin-2 (AQP2). Here we present evidence that vasopressin may also act as a long-term, transcriptional regulator AQP2. The studies were performed on LLC-PK1 cells, which normally express V2 receptor (V2R) and transfected with fragment human AQP2 promoter. Activation adenylate cyclase-coupled V2R in cells induced phosphorylation adenosine 3',5'-cyclic monophosphate...
Recent studies indicate novel roles for the ubiquitous ion pump, Na,K-ATPase, in addition to its function as a key regulator of intracellular sodium and potassium concentration. We have previously demonstrated that ouabain, endogenous ligand can trigger Ca2+ oscillations, versatile signal controlling diverse range cellular processes. Here we report Na,K-ATPase inositol 1,4,5-trisphosphate (InsP3) receptor (InsP3R) form cell signaling microdomain that, presence generates slow oscillations...
The coupling between the Na+/glucose cotransporter and Na+-K+-ATPase (NKA) described for epithelial cells (1) prompted us to study in rats with streptozocin-induced diabetes effect of increased tubular glucose load on Na+ reabsorption, NKA-dependent O2 consumption (QO2), NKA activity. Filtered is mainly reabsorbed proximal tubuli via phlorizin-sensitive cotransporter. In this study, diabetic had a significantly higher renal blood flow (RBF), glomerular filtration rate (GFR), reabsorption...
Glutamate, by activating N -methyl- d -aspartate (NMDA) receptors, alters the balance between dopamine D1 and D2 receptor signaling, but mechanism responsible for this effect has not been known. We report here, using immunocytochemistry of primary cultures rat neostriatal neurons, that activation NMDA receptors recruits from interior cell to plasma membrane while having no on distribution receptors. The were concentrated in spines as shown colocalization with phalloidin-labeled actin...