A5077235220- Mitochondrial Function and Pathology
- Retinal Development and Disorders
- Neonatal Health and Biochemistry
- Particle physics theoretical and experimental studies
- Particle Detector Development and Performance
- Erythrocyte Function and Pathophysiology
- ATP Synthase and ATPases Research
- Retinal Diseases and Treatments
- Photoreceptor and optogenetics research
- Cellular transport and secretion
- High-Energy Particle Collisions Research
- Metabolism and Genetic Disorders
- Photosynthetic Processes and Mechanisms
- Hemoglobin structure and function
- Neuroscience and Neuropharmacology Research
- Lipid Membrane Structure and Behavior
- Adenosine and Purinergic Signaling
- Pancreatic function and diabetes
- Ion channel regulation and function
- Venomous Animal Envenomation and Studies
- Radiation Detection and Scintillator Technologies
- Electromagnetic Fields and Biological Effects
- Glaucoma and retinal disorders
- Heme Oxygenase-1 and Carbon Monoxide
- Connexins and lens biology
University of Genoa
2015-2025
Istituto Nazionale di Fisica Nucleare, Sezione di Genova
1995-2009
University of Perugia
1985-2005
University of Pisa
2001
Istituto Nazionale di Fisica Nucleare
1986-1998
Istituto Nazionale di Fisica Nucleare, Sezione di Roma I
1993-1997
GTx (United States)
1997
Istituto di Chimica Biomolecolare
1993-1997
École Polytechnique Fédérale de Lausanne
1989
Mayo Clinic
1989
The TOTEM Experiment will measure the total pp cross-section with luminosity-independent method and study elastic diffractive scattering at LHC. To achieve optimum forward coverage for charged particles emitted by collisions in interaction point IP5, two tracking telescopes, T1 T2, be installed on each side pseudorapidity region 3.1 ⩽ |η| 6.5, Roman Pot stations placed distances of ±147 m ±220 from IP5. Being an independent experiment but technically integrated into CMS, first operate...
N-acetylcysteine (NAC) was administered to rats in various combinations with an enzyme inducer (Aroclor 1254) and depletors of reduced glutathione (GSH), i.e., diethyl maleate (DEM) buthionine sulfoximine (BSO). NAC increased intracellular levels erythrocytes liver lung cells, replenished its stores following depletion. It did not affect the concentrations nor spectral properties cytochromes P-450 hepatic pulmonary microsomes, whereas it stimulated, especially Aroclor-pre-treated animals,...
The process of cellular respiration occurs for energy production through catabolic reactions, generally with glucose as the first step. In present work, we introduce a novel concept understanding this process, based on our conclusion that metabolism is coupled to pentose phosphate pathway (PPP) and extra-mitochondrial oxidative phosphorylation in closed-loop process. According current standard model glycolysis, converted 6-phosphate (glucose 6-P) then fructose 6-phosphate, glyceraldehyde...
Abstract The activity of the membrane-bound and purified erythrocyte Ca2+-ATPase in absence calmodulin was stimulated by calpain digestion but could be further increased to maximal levels (CaM). Thus, CaM sensitivity retained digested ATPase, at least short times incubation. In membranes higher temperatures ATPase calpain/ATPase ratios, became fully activated. 138-kDa were converted a fragment approximately 124 kDa which still bound isolated on columns when proteolysis occurred slowly not it...
A solid-phase radioimmunoassay for human glucose-6-phosphate dehydrogenase (D-glucose-6-phosphate: NADP+ 1-oxidoreductase; EC 1.1.1.49) was developed that allowed the specific activity of this enzyme protein to be measured in lysates from whole erythrocyte populations, erythrocytes different ages, and purified samples. The highly single donors by a simple procedure affinity chromatography with insolubilized adenosine 2',5'-bisphosphate. These techniques were used an attempt elucidate...
Oxidative phosphorylation (OXPHOS) is not only the main source of ATP for cell, but also a major reactive oxygen species (ROS), which lead to oxidative stress. At present, mitochondria are considered organelles responsible OXPHOS, in last years we have demonstrated that it can occur outside mitochondrion. Myelin sheath able conduct an aerobic metabolism, producing hypothesized transferred axon, support its energetic demand. In this work, spectrophotometric, cytofluorimetric, and luminometric...
N-Acetylcysteine (NAC), reduced (GSH) and oxidized (GSSG) glutathione were negative in the Ames test with 7 Salmonella strains, while L-cysteine was activated by rat liver S-9 fractions to metabolites mutagenic strains TA102, TA97 TA100. The response S. typhimurium (TA1535, TA98, TA100, TA102) levels of enzyme activities, responsible for NADP+ or GSSG reduction utilization NADPH GSH fractions, investigated following vitro preincubation NAC four direct-acting mutagens six procarcinogens....
Red blood cells of favism patients with acute hemolytic crisis have markedly more superoxide dismutase (superoxide: oxidoreductase, EC 1.15.1.1) and less glutathione peroxidase (glutathione: hydrogen‐peroxide 1.11.1.9) than either normal controls, glucose‐6‐phosphate dehydrogenase‐deficient subjects or outside crisis. This altered value the two enzyme activities is not due to increased reticulocyte content blood. The electrophoretic triplet pattern also changed, significant increase most...
1. The distribution of proteolytic activity in membranes from human erythrocytes and rabbit reticulocytes was investigated, after removal leucocytes platelets the cell suspensions. 2. All membrane preparations displayed acidic pH region only. Membranes mature showed latent activity, which could be increased when extracted with a number detergents. 3. Three active fractions were resolved either by gel chromatography solubilized extracts or standard polyacrylamide-gel electrophoresis. three...
The initial events of vision at low light take place in vertebrate retinal rods. rod outer segment consists a stack flattened disks surrounded by the plasma membrane. A list proteins that reside has not been achieved yet. We present first comprehensive proteomic analysis purified disks, obtained combining results two-dimensional gel electrophoresis separation disk to MALDI-TOF or nLC-ESI-MS/MS mass spectrometry techniques. Intact were isolated from bovine segments method minimizes...
Individual lysates from human erythrocyte suspensions, completely deprived of leucocytes and platelets, were assayed for a number proteolytic activities using both naturally occurring synthetic substrates. Removal hemoglobin by batchwise DEAE‐cellulose chromatography did not modify the complement various which then fractionated means on column DEAE‐cellulose, followed conventional techniques such as gel preparative electrophoresis. This procedure allowed proteinases to be identified in...