A5075384838- Advanced Proteomics Techniques and Applications
- Ubiquitin and proteasome pathways
- Extracellular vesicles in disease
- RNA Research and Splicing
- Autophagy in Disease and Therapy
- Mass Spectrometry Techniques and Applications
- DNA Repair Mechanisms
- Endoplasmic Reticulum Stress and Disease
- Cellular transport and secretion
- RNA modifications and cancer
- Protein Degradation and Inhibitors
- Immunotherapy and Immune Responses
- Metabolomics and Mass Spectrometry Studies
- Cell Adhesion Molecules Research
- Monoclonal and Polyclonal Antibodies Research
- Peptidase Inhibition and Analysis
- Glycosylation and Glycoproteins Research
- CAR-T cell therapy research
- Phagocytosis and Immune Regulation
- Genetics and Neurodevelopmental Disorders
- RNA and protein synthesis mechanisms
- Retinal Development and Disorders
- Nuclear Structure and Function
- MicroRNA in disease regulation
- Cancer, Hypoxia, and Metabolism
IFOM
2015-2024
Vita-Salute San Raffaele University
2006-2018
San Raffaele University of Rome
2005-2015
Istituti di Ricovero e Cura a Carattere Scientifico
2015
Istituto di Ricovero e Cura a Carattere Scientifico San Raffaele
2015
Mylan (Switzerland)
2015
IRCCS Ospedale San Raffaele
2003-2014
University of Tartu
2012
Marin Community Foundation
2010
Mercedes-Benz (Germany)
2010
Tissue damage causes inflammation, by recruiting leukocytes and activating them to release proinflammatory mediators. We show that high-mobility group box 1 protein (HMGB1) orchestrates both processes switching among mutually exclusive redox states. Reduced cysteines make HMGB1 a chemoattractant, whereas disulfide bond makes it cytokine further cysteine oxidation sulfonates reactive oxygen species abrogates activities. leukocyte recruitment activation can be separated. A nonoxidizable mutant...
After tissue damage, inflammatory cells infiltrate the and release proinflammatory cytokines. HMGB1 (high mobility group box 1), a nuclear protein released by necrotic severely stressed cells, promotes cytokine via its interaction with TLR4 (Toll-like receptor 4) cell migration an unknown mechanism. We show that HMGB1-induced recruitment of depends on CXCL12. CXCL12 form heterocomplex, which we characterized magnetic resonance surface plasmon resonance, acts exclusively through CXCR4 not...
Formation of mixed disulfides between glutathione and the cysteines some proteins (glutathionylation) has been suggested as a mechanism through which protein functions can be regulated by redox status. The aim this study was to identify T cell blasts that undergo glutathionylation under oxidative stress. To purpose, we radiolabeled cellular with 35 S, exposed cells oxidants (diamide or hydrogen peroxide), performed nonreducing, two-dimensional electrophoresis followed detection labeled...
Abstract Purpose: Glioblastoma (GBM) is the most common primary brain tumor. The identification of blood biomarkers reflecting tumor status represents a major unmet need for optimal clinical management patients with GBM. Their high number in body fluids, their stability, and presence many tumor-associated proteins RNAs make extracellular vesicles potentially biomarkers. Here, we investigated potential role plasma from GBM diagnosis follow-up after treatment as prognostic tool. Experimental...
Vertebrate TAP and its yeast ortholog Mex67p are involved in the export of messenger RNAs from nucleus. has also been implicated simian type D viral bearing constitutive transport element (CTE). Although directly interacts with CTE-bearing RNAs, mode interaction TAP/Mex67p cellular mRNAs is different that CTE RNA likely to be mediated by protein-protein interactions. Here we show Yra1p, an essential hnRNP-like protein. This evolutionarily conserved as Yra1p TAP. Conditional expression cells...
Messenger RNAs are exported from the nucleus as large ribonucleoprotein complexes (mRNPs). To date, proteins implicated in this process include TAP/Mex67p and RAE1/Gle2p distinct nuclear transport receptors of β-related, Ran-binding protein family. Mex67p is essential for mRNA export yeast. Its vertebrate homolog TAP has been cellular mRNAs simian type D viral bearing constitutive element (CTE). Here we show that predominantly localized nucleoplasm at both nucleoplasmic cytoplasmic faces...
CRM1 is an export receptor mediating rapid nuclear exit of proteins and RNAs to the cytoplasm. cargoes include with a leucine-rich signal (NES) that bind directly in trimeric complex RanGTP. Using quantitative CRM1-NES cargo binding assay, significant differences affinity for among natural NESs are demonstrated, suggesting steady-state nucleocytoplasmic distribution shuttling could be determined by relative strengths their NESs. We also show CRM1-NES-RanGTP disassembled RanBP1 presence...
Isoaspartate formation in extracellular matrix proteins, by aspartate isomerization or asparagine deamidation, is generally viewed as a degradation reaction occurring <i>in vivo</i> during tissue aging. For instance, non-enzymatic isoaspartate at RGD-integrin binding sites causes loss of cell adhesion sites, which turn can be enzymatically "repaired" to RGD protein-l-<i>iso</i>Asp-<i>O</i>-methyltransferase. We show here that also mechanism for activation. In particular, we deamidation...
Lifelong blood cell production is governed through the poorly understood integration of cell-intrinsic and -extrinsic control hematopoietic stem (HSC) quiescence activation. MicroRNAs (miRNAs) coordinately regulate multiple targets within signaling networks, making them attractive candidate HSC regulators. We report that miR-126, a miRNA expressed in early progenitors, plays pivotal role restraining cell-cycle progression vitro vivo. miR-126 knockdown by using lentiviral sponges increased...
The mechanism of neurodegeneration caused by beta-amyloid in Alzheimer disease is controversial. Neuronal toxicity exerted mostly various species soluble oligomers that differ their N- and C-terminal domains. However, abundant accumulation also occurs the brains cognitively normal elderly people, absence obvious neuronal dysfunction. We postulated depends on molecular composition, rather than amount, oligomers. Here we show aggregates accumulate are different from those aging regard to...