A5082582019- Extracellular vesicles in disease
- Cellular transport and secretion
- MicroRNA in disease regulation
- Nuclear Structure and Function
- Autophagy in Disease and Therapy
- RNA modifications and cancer
- Ubiquitin and proteasome pathways
- Microtubule and mitosis dynamics
- Cancer, Hypoxia, and Metabolism
- Retinal Development and Disorders
- Ferroptosis and cancer prognosis
- Lipid Membrane Structure and Behavior
- DNA Repair Mechanisms
- Circular RNAs in diseases
- Pancreatic function and diabetes
- Genetic Syndromes and Imprinting
- Genetic and Kidney Cyst Diseases
- Nanopore and Nanochannel Transport Studies
- GDF15 and Related Biomarkers
- Macrophage Migration Inhibitory Factor
- Renal and related cancers
- Endoplasmic Reticulum Stress and Disease
- Urticaria and Related Conditions
- Biotin and Related Studies
- RNA Research and Splicing
IFOM
2013-2024
Mario Negri Sud Foundation
2013
ATR controls chromosome integrity and chromatin dynamics. We have previously shown that yeast Mec1/ATR promotes detachment from the nuclear envelope to counteract aberrant topological transitions during DNA replication. Here, we provide evidence activity at responds mechanical stress. Human associates with S phase prophase, both osmotic stress stretching relocalize membranes throughout cell cycle. The ATR-mediated response occurs within range of physiological forces, is reversible,...
Abstract Purpose: Glioblastoma (GBM) is the most common primary brain tumor. The identification of blood biomarkers reflecting tumor status represents a major unmet need for optimal clinical management patients with GBM. Their high number in body fluids, their stability, and presence many tumor-associated proteins RNAs make extracellular vesicles potentially biomarkers. Here, we investigated potential role plasma from GBM diagnosis follow-up after treatment as prognostic tool. Experimental...
Abstract ATR responds to mechanical stress at the nuclear envelope and mediates envelope-associated repair of aberrant topological DNA states. By combining microscopy, electron microscopic analysis, biophysical in vivo models, we report that ATR-defective cells exhibit altered plasticity YAP delocalization. When subjected or undergoing interstitial migration, nuclei collapse accumulating ruptures perinuclear cGAS, which indicate loss integrity, chromatin status. also are defective neuronal...
// Matteo Setti 1, * , Daniela Osti Cristina Richichi 1 Barbara Ortensi Massimiliano Del Bene 2 Lorenzo Fornasari Galina Beznoussenko 3 Alexandre Mironov Germana Rappa Alessandro Cuomo Mario Faretta Tiziana Bonaldi Aurelio Lorico 4 Giuliana Pelicci 5 Department of Experimental Oncology, European Institute Milan, Italy Neurosurgery, Fondazione IRCCS Istituto Neurologico C. Besta, Molecular Oncology (IFOM) the Italian Foundation for Cancer Research (FIRC), Center, Roseman University Health...
The mechanism of transport through the Golgi complex is not completely understood, insofar as no single appears to account for all observations. Here, we compare soluble secretory proteins (albumin and α1-antitrypsin) with that supramolecular cargoes (e.g., procollagen) are proposed traverse by compartment progression–maturation. We show these much faster than procollagen while moving same stack. Moreover, present kinetic morphological observations indicate albumin occurs diffusion via...
How autophagic degradation is linked to endosomal trafficking routes little known. Here we screened a collection of uncharacterized Drosophila mutants affecting membrane transport identify new genes that also have role in autophagy. We isolated loss function mutant Snap29 (Synaptosomal-associated protein 29 kDa), the gene encoding homolog human SNAP29 and characterized its vivo. contains 2 soluble NSF attachment receptor (SNARE) domains asparagine-proline-phenylalanine (NPF motif) at N...
// Aleco D'Andrea 1 , Ilaria Gritti 1,4 Paola Nicoli Marco Giorgio Mirko Doni Annalisa Conti 2 Valerio Bianchi 2,5 Lucia Casoli Arianna Sabò Alexandre Mironov 3 Galina V. Beznoussenko and Bruno Amati 1,2 Department of Experimental Oncology, European Institute Milan, Italy Center for Genomic Science IIT@SEMM, Fondazione Istituto Italiano di Tecnologia, The Molecular Oncology the Italian Foundation Cancer Research, 4 Present address: IRCCS San Raffaele, Functional Genomics Unit, Division 5...
Strategies aimed at obtaining a complete cytoreduction are needed to improve long-term survival for patients with colorectal cancer peritoneal carcinomatosis (CRC-pc). We established organoid models from metastases of two naïve CRC patients. A standard paraffin inclusion was conducted compare their 3D structure and immunohistochemical profile that the corresponding surgical samples. RNA expression levels stem cell marker LGR5 measured by in situ hybridization. The secretome organoids...
The Golgi apparatus is the main glycosylation and sorting station along secretory pathway. Its structure includes vesicles, which are depleted of anterograde cargo, also at least some Golgi‐resident proteins. role vesicles remains unclear. Here, we show that enriched in Qb‐ SNAREs GS27 (membrin) GS28 ( GOS ‐28), nucleotide sugar transporters. A block intra‐Golgi transport leads to accumulation partitioning into these vesicles. Conversely, active induces fusion with cisternae, delivering...
Homozygous mutations in SNAP29, encoding a SNARE protein mainly involved membrane fusion, cause CEDNIK (Cerebral Dysgenesis, Neuropathy, Ichthyosis and Keratoderma), rare congenital neurocutaneous syndrome associated with short life expectancy, whose pathogenesis is unclear. Here, we report the analysis of first genetic model zebrafish. Strikingly, homozygous snap29 mutant larvae display CEDNIK-like features, such as microcephaly skin defects. Consistent Snap29 role fusion during autophagy,...
Abstract The kinetochore is an essential structure that mediates accurate chromosome segregation in mitosis and meiosis. While many of the components have been identified, mechanisms assembly remain elusive. Here, we identify a novel role for Snap29, unconventional SNARE , promoting during Drosophila human cells. Snap29 localizes to outer prevents mis‐segregation formation cells with fragmented nuclei. promotes by mediating recruitment Knl1 at ensuring stable microtubule attachments. Correct...
The actin motor protein myosin VI is a multivalent with diverse functions.Here, we identified and characterised ubiquitous interactor, the oral-facial-digital syndrome 1 (OFD1) protein, whose mutations cause malformations of face, oral cavity, digits polycystic kidney disease.We found that regulates localisation OFD1 at centrioles and, as consequence, recruitment distal appendage Cep164.Myosin depletion in non-tumoural cell lines causes an aberrant along centriolar walls, which due to...
<title>Abstract</title> Micronuclei (MN) can arise from many causes, including the breakage of aberrant cytokinetic chromatin bridge. The frequent observation MN in tumors raises specter that they might not merely be passive elements but could instead play active roles tumor progression. Here, we propose a mechanism presence micronuclei induce specific phenotypic and functional changes to cell lead increased cancer invasive potential. Through integration diverse imaging molecular techniques...
"Early autophagosomes are formed from myelin-like structures derived outer membranes of mitochondria." Ultrastructural Pathology, 41(1), pp. 73–74
Abstract The actin motor protein myosin VI is a multivalent with diverse functions. Here, we identified and characterised ubiquitous interactor, the oral-facial-digital syndrome 1 (OFD1) protein, whose mutations cause malformations of face, oral cavity, digits, polycystic kidney disease. We found that regulates localisation OFD1 at centrioles and, as consequence, recruitment distal appendage cep164. Myosin depletion in non-tumoural cell lines causes an aberrant along centriolar walls, which...
Glioblastoma (GBM) is the most common primary brain tumor. Despite aggressive treatment (surgery, chemo- and radiotherapy), prognosis dismal (median overall survival: 14m). Actually, early diagnosis monitoring represent major unmet needs. Extracellular vesicles (EVs) are potentially optimal biomarkers owing to high stability their presence in blood cerebrospinal fluid. These features, combined genetic proteomic composition that mirrors intratumoral environment, highlight EV applicability as...
<p>Supplementary Figures and Tables Captions</p>
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<div>AbstractPurpose:<p>Glioblastoma (GBM) is the most common primary brain tumor. The identification of blood biomarkers reflecting tumor status represents a major unmet need for optimal clinical management patients with GBM. Their high number in body fluids, their stability, and presence many tumor-associated proteins RNAs make extracellular vesicles potentially biomarkers. Here, we investigated potential role plasma from GBM diagnosis follow-up after treatment as prognostic...
<div>AbstractPurpose:<p>Glioblastoma (GBM) is the most common primary brain tumor. The identification of blood biomarkers reflecting tumor status represents a major unmet need for optimal clinical management patients with GBM. Their high number in body fluids, their stability, and presence many tumor-associated proteins RNAs make extracellular vesicles potentially biomarkers. Here, we investigated potential role plasma from GBM diagnosis follow-up after treatment as prognostic...