A5031476784- Cellular Mechanics and Interactions
- Colorectal Cancer Treatments and Studies
- Cancer, Hypoxia, and Metabolism
- Cancer Treatment and Pharmacology
- Vascular Malformations Diagnosis and Treatment
- 3D Printing in Biomedical Research
- Phagocytosis and Immune Regulation
- Protein Kinase Regulation and GTPase Signaling
- Hippo pathway signaling and YAP/TAZ
- Cancer Research and Treatments
- Cancer Genomics and Diagnostics
- Cancer Cells and Metastasis
- Vascular Tumors and Angiosarcomas
- Endoplasmic Reticulum Stress and Disease
- Neuroscience and Neural Engineering
- Vascular Malformations and Hemangiomas
- Cell death mechanisms and regulation
Candiolo Cancer Institute
2015-2024
University of Turin
2017-2020
Istituti di Ricovero e Cura a Carattere Scientifico
2015
Abstract Somatic activating mutations within the PIK3CA gene have been recently detected in sporadic lymphatic and venous malformations, vascular malformations (VM) associated to overgrowth syndromes, such as CLOVES Klippel–Trenaunay syndrome. Although VM are often limited specific tissue areas can be well treated, extended or recurrent lesions novel therapeutic approaches needed. We generated a mouse model of by local expression PIK3CA- mutation endothelial cells. driven characterized large...
Abstract Cellular protrusions are highly dynamic structures involved in fundamental processes,including cell migration and invasion. For a to migrate, its leading edge mustform then adhere or retract. The spatial temporal coordinationof retraction is yet be fully understood. study of protrusiondynamics mainly relies on live-microscopy often coupled fluorescent labeling.Here we report the use an alternative, label-free, quantitative rapid assayto analyze protrusion dynamics population based...
Extrusion of apoptotic cells from epithelial tissues requires orchestrated morphological rearrangements the cell and its neighbors. However, connections between cascade events leading to extrusion are not fully understood. Here, we characterize an apical actin ring (EAAR) that is assembled within drives extrusion. Caspase-mediated cleavage myotonic dystrophy kinase–related CDC42-binding kinase-α (MRCKα) triggers a signaling pathway leads assembly EAAR pulls bundles, resulting in compaction...
Abstract The activation of the majority AGC kinases is regulated by two phosphorylation events on conserved serine/threonine residues located loop and hydrophobic motif, respectively. In kinase family, phosphomimetic substitutions with aspartate or glutamate, leading to constitutive activation, have frequently occurred at motif site. On contrary, in are absent across evolution kinases. This observation explained failure glutamate mimic phosphorylatable this regulatory By detailed 3D...
The breadth and depth at which cancer models are interrogated contribute to the successful clinical translation of drug discovery efforts. In colorectal (CRC), model availability is limited by a dearth large-scale collections patient-derived xenografts (PDXs) paired tumoroids from metastatic disease, where experimental therapies typically tested. Here we introduce XENTURION, an open-science resource offering platform 128 PDX patients with CRC, along matched PDX-derived tumoroids....
ABSTRACT The breadth and depth at which cancer models are interrogated contribute to successful translation of drug discovery efforts the clinic. In colorectal (CRC), model availability is limited by a dearth large-scale collections patient-derived xenografts (PDXs) paired tumoroids from metastatic disease, setting where experimental therapies typically tested. XENTURION unique open-science resource that combines platform 129 PDX sister matched PDX-derived (PDXTs) patients with CRC,...
Abstract 3-Phosphoinositide Dependent Kinase 1 (PDK1) is the kinase that phosphorylates activation loop of several protein kinases such as Akt, PKC, RSK or SGK. In melanoma, breast and prostate carcinomas PDK1 was found amplified overexpressed this event correlates with a more aggressive phenotype. The purpose our study to unveil mechanism by which regulates cell migration invasion aim rationally target it in order prevent tumor dissemination. Combining stable silencing overexpression, we...