A5082641413- Enzyme Structure and Function
- Microbial Applications in Construction Materials
- Enzyme Production and Characterization
- Educational and Social Studies
- Plant Pathogenic Bacteria Studies
- Biochemical and Molecular Research
- Microbial Metabolites in Food Biotechnology
- Enzyme function and inhibition
- Photosynthetic Processes and Mechanisms
- Bacteriophages and microbial interactions
- Plant nutrient uptake and metabolism
- Peptidase Inhibition and Analysis
- Genomics and Phylogenetic Studies
- Plant-Microbe Interactions and Immunity
- Microbial Metabolism and Applications
- Diet, Metabolism, and Disease
- Protein Structure and Dynamics
- Glycosylation and Glycoproteins Research
- Corrosion Behavior and Inhibition
- Biofuel production and bioconversion
- Cancer Research and Treatments
- RNA and protein synthesis mechanisms
- Metal complexes synthesis and properties
- Mycotoxins in Agriculture and Food
- Amino Acid Enzymes and Metabolism
Free University of Bozen-Bolzano
2014-2024
University of Bologna
1996-2021
University of York
2000-2011
AstraZeneca (United Kingdom)
2008
International Centre for Genetic Engineering and Biotechnology
2001-2004
University of Siena
2004
Institute of Bioorganic Chemistry, Polish Academy of Sciences
2004
European Bioinformatics Institute
2001
European Molecular Biology Laboratory
1999-2000
European Molecular Biology Laboratory
1998-2000
Transition metals are both essential to enzymatic catalysis and limited in environmental availability. These two biological facts have together driven organisms evolve mechanisms for selective metal ion sensing utilization. Changes concentrations perceived by metal-dependent transcription factors transduced into appropriate cellular responses, which regulate the machineries of competitive homeostasis metallo-enzyme activation. The intrinsic toxicity majority ions further creates a need...
Abstract Urease, the most efficient enzyme known, contains an essential dinuclear Ni II cluster in active site. It catalyzes hydrolysis of urea, inducing a rapid pH increase that has negative effects on human health and agriculture. Thus, control urease activity is utmost importance medical, pharmaceutical, agro‐environmental applications. All known inhibitors are either toxic or inefficient. The development new chemicals able to inhibit relies knowledge all steps catalytic mechanism. short...
The structure of the complex urease, a Ni-containing metalloenzyme, with boric acid was determined at 2.10 Å resolution. shows unprecedented binding mode competitive inhibitor to dinuclear metal center, B(OH)3 molecule bridging Ni ions and leaving in place hydroxide. Boric can be considered substrate analogue urea, supports proposal that Ni-bridging hydroxide acts as nucleophile enzymatic process urea hydrolysis.
Genome packaging into preformed viral procapsids is driven by powerful molecular motors. The small terminase protein essential for the initial recognition of DNA and regulates motor’s ATPase nuclease activities during translocation. crystal structure a full-length from Siphoviridae bacteriophage SF6, comprising N-terminal binding, oligomerization core, C-terminal β-barrel domains, reveals nine-subunit circular assembly in which DNA-binding domains are arranged around core highly flexible...
Erwinia amylovora is a plant pathogen that affects Rosaceae, such as apple and pear. In E. the fructans, produced by action of levansucrase (EaLsc), play role in virulence biofilm formation. Fructans are bioactive compounds, displaying health-promoting properties their own right. Their use food feed supplements increasing. this study, we investigated biomolecular EaLsc using HPAEC-PAD, MALDI-TOF MS, spectrophotometric assays. The enzyme, which was heterologously expressed Escherichia coli...
Abstract Urease uses a cluster of two Ni II ions to activate water molecule for urea hydrolysis. The key this unsurpassed enzyme is change in the conformation flexible structural motif, mobile flap, which must be able move from an open closed stabilize chelating interaction with cluster. This conformational brings imidazole side chain functionality critical histidine residue, αHis323, close proximity site that holds transition state structure reaction, facilitating its evolution products....
Several studies have shown that mammalian retinal rod outer segments (OS) are peculiar structures devoid of mitochondria, characterized by ectopic expression the molecular machinery for oxidative phosphorylation. Such aerobic metabolism would provide chemical energy phototransduction taking place in OS. Natural polyphenols include a large variety molecules having pleiotropic effects, ranging from anti-inflammatory to antioxidant and others. Our goal present study was investigate potential...
This article reports the first X-ray structure of soluble form a c-type cytochrome isolated from Gram-positive bacterium. Bacillus pasteurii c553, characterized by low reduction potential and sequence homology with cytochromes Gram-negative bacteria or eukaryotes, is useful case study for understanding structure−function relationships this class electron-transfer proteins. Diffraction data on single crystal c553 were obtained using synchrotron radiation at 100 K. The was determined 0.97-Å...
The Erwinia genus comprises species that are plant pathogens, non-pathogen, epiphytes, and opportunistic human pathogens. Within the genus, amylovora ranks among top 10 pathogenic bacteria. It causes fire blight disease is a global threat to commercial apple pear production. We analyzed presence/absence of E. genes reported be important for pathogenicity towards Rosaceae within various strains genomes. This simple bottom-up approach, allowed us correlate pathogenicity, host specificity, make...
The crystal structure of Helicobacter pylori holo-NikR, a Ni2+-dependent transcription factor, determined at pH 7.3, shows four square-planar nickel-binding sites, involving one cysteinate and three histidine ligands. This observation reconciles previous inconsistencies among calorimetric data, structural information non-physiological pH, computational studies.
Glucose-1-phosphate uridylyltransferase in conjunction with UDP-glucose pyrophosphorylase was found to catalyse the conversion of a range 5-substituted UTP derivatives into corresponding UDP-galactose poor yield. Notably 5-iodo derivative not converted UDP-sugar. In contrast, inorganic pyrophosphatase particularly effective at converting derivatives, including iodo compound, gluco-configured UDP-sugar good yields. Attempts effect 4″-epimerization these 4″-epimerase from yeast were...