A5013758426- Bone Metabolism and Diseases
- Estrogen and related hormone effects
- Mesenchymal stem cell research
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- RNA Research and Splicing
- RNA Interference and Gene Delivery
- Osteoarthritis Treatment and Mechanisms
- Bone health and treatments
- MicroRNA in disease regulation
- DNA and Nucleic Acid Chemistry
- NF-κB Signaling Pathways
- Genomics and Chromatin Dynamics
- Cancer-related molecular mechanisms research
- Genetic and Kidney Cyst Diseases
- Tissue Engineering and Regenerative Medicine
- Bone and Dental Protein Studies
- 3D Printing in Biomedical Research
- RNA modifications and cancer
- Cell Adhesion Molecules Research
- Bone Tissue Engineering Materials
- Retinoids in leukemia and cellular processes
- Advanced biosensing and bioanalysis techniques
- Molecular Biology Techniques and Applications
- T-cell and B-cell Immunology
University of Ferrara
2016-2025
University of Genoa
2018
University of Bonn
2017
In-Q-Tel
2014
Center for Translational Neurophysiology of Speech and Communication
2011
Micron (Italy)
2009
Institute of Molecular Biology
2007
Institute of Clinical and Experimental Medicine
2006
Azienda Unità Sanitaria Locale di Ferrara
1999
Albert Einstein College of Medicine
1999
There is a growing demand for the development of experimental strategies efficient articular cartilage repair. Current tissue engineering-based regenerative make use human mesenchymal stromal cells (hMSCs). However, when implanted in defect, control hMSCs differentiation toward chondrogenic lineage remains significant challenge. We have recently demonstrated that silencing antichondrogenic regulator microRNA-221 (miR-221) was highly effective promoting vitro chondrogenesis monolayered...
In order to study the pathogenesis of gingival overgrowth induced by immuno‐suppressive drug cyclosporine‐A (CyA), we investigated its effect on 3 H thymidine incorporation and collagen production mRNA levels in fibroblast cultures obtained from normal human gingiva. At concentrations 100, 500 1000 ng/ml, CyA did not modify after 24 72 h incubation. However, it significantly increased level proline‐containing proteins medium. addition, α‐procollagen chains up three times. This CyA‐induced...
Abstract Background Osteoclasts (OCs) are involved in rheumatoid arthritis and several pathologies associated with bone loss. Recent results support the concept that some medicinal plants derived natural products of great interest for developing therapeutic strategies against disorders, including osteoporosis. In this study we determined whether extracts Emblica officinalis fruits display activity possible treatment osteoporosis by activating programmed cell death human primary osteoclasts....
The description of a microencapsulation procedure for Wharton's jelly mesenchymal stem cells (WJMSCs) is reported. applied method based on the generation monodisperse droplets by vibrational nozzle. An ionic alginate encapsulation was utilized microbeads hardening. Different experimental parameters were analyzed, including frequency and amplitude vibration, polymer pumping rate, distance between nozzle gelling bath. produced barium-alginate characterized excellent morphological...
Abstract Background Breast cancer and its metastatic progression is mainly directed by epithelial to mesenchymal transition (EMT), a phenomenon supported specific transcription factors miRNAs. Methods In order investigate possible correlation between Slug factor miR-221, we performed gene silencing in MDA-MB-231 breast cells evaluated the expression of genes involved supporting phenotype, using qRT-PCR Western blot analysis. Chromatin immunoprecipitation wound healing assays were employed...
The aim of this study was to investigate the role an antichondrogenic factor, MIR221 (miR-221), in intervertebral disc degeneration (IDD), and provide basic information for development a therapeutic strategy repair based on specific nucleic acid drugs, such as miR-221 silencing.We established relatively quick protocol minimize artifacts from extended vitro culture, without selecting different types cells (IVD) or completely disrupting extracellular matrix (ECM), but by using whole cell...
Regenerative therapies for intervertebral disc (IVD) injuries are currently a major challenge that is addressed in different ways by scientists working this field. Extracellular matrix (ECM) deriving from decellularized non-autologous tissues has been established as biomaterial with remarkable regenerative capacity and its potential therapeutic agent rising. In the present study, we investigated of Wharton's jelly (DWJM) human umbilical cord to act an ECM-based scaffold IVD cell culturing....
Polycystin-2 (PC2), encoded by the PKD2 gene, mutated in 10-15% of autosomal-dominant polycystic kidney disease (ADPKD) patients, is a Ca2+-permeable cation channel present epithelia and other tissues. As PC2 was found expressed B-lymphoblastoid cells (LCLs) Ca2+ signaling pathways are important regulators B cell function activities, we investigated whether plays some role B-LCLs. In LCLs, mainly ER membranes but ~8 times less than HEK293 cells. The same reductions were PKD1 RNA; thus, PKD...
This paper describes a method for the production of alginate microfibres using glass-based microfluidic chips fabricated by photolithography-wet etching procedure. The main focus work is fabrication cell containing multifunctional which have great potential applications in drug release formulations and tissue engineering scaffolds (to guide regeneration tissues predefined sizes shapes) providing structural support immunoisolation. key parameters, critically influence formation their...
Journal Article Dinucleotide repeat polymorphism in the human estrogen receptor (ESR) gene Get access L.del Senno, Senno * Istituto di Chimica Biologica, Centro Studi Biochimici delle Patologie del Genoma Umano, Università FerraraItaly *To whom correspondence should be addressed Search for other works by this author on: Oxford Academic PubMed Google Scholar G.L. Aguiari, Aguiari R. Piva Human Molecular Genetics, Volume 1, Issue 5, August 1992, Page 354, https://doi.org/10.1093/hmg/1.5.354...
Abstract Background Lymphoid Enhancer Factor-1 (Lef-1) is a member of transcription factor family that acts as downstream mediator the Wnt/β-catenin signalling pathway which plays critical role in osteoblast proliferation and differentiation. In search for Lef-1 responsive genes human osteoblasts, we focused on transcriptional regulation SLUG, zinc finger belonging to Snail developmental proteins. Although SLUG epithelial-mesenchymal transition cell motility during embryogenesis well...
The pathways that control mesenchymal stem cells (MSCs) differentiation are not well understood, and although some of the involved transcription factors (TFs) have been characterized, role others remains unclear. We used human MSCs from tibial plateau (TP) trabecular bone, iliac crest (IC) bone marrow Wharton's jelly (WJ) umbilical cord demonstrating a variability in their mineral matrix deposition, expression levels TFs including Runx2, Sox9, Sox5, Sox6, STAT1 Slug, all...