A5070164014- Sperm and Testicular Function
- Pancreatic function and diabetes
- Reproductive Biology and Fertility
- Xenotransplantation and immune response
- Heavy Metal Exposure and Toxicity
- Tissue Engineering and Regenerative Medicine
- Diabetes Management and Research
- Diabetes and associated disorders
- Electrospun Nanofibers in Biomedical Applications
- Muscle Physiology and Disorders
- RNA Interference and Gene Delivery
- Growth Hormone and Insulin-like Growth Factors
- Reproductive System and Pregnancy
- 3D Printing in Biomedical Research
- Infant Nutrition and Health
- Ovarian function and disorders
- Nerve injury and regeneration
- Cannabis and Cannabinoid Research
- Adipose Tissue and Metabolism
- Renal Diseases and Glomerulopathies
- Selenium in Biological Systems
- Microfluidic and Bio-sensing Technologies
- Carbohydrate Chemistry and Synthesis
- Extracellular vesicles in disease
- Testicular diseases and treatments
University of Perugia
2015-2024
University of Naples Federico II
2002-2012
General Department of Preventive Medicine
2009
William Harvey Research Institute
1995
St Bartholomew's Hospital
1995
Time-related decline of human islet allograft (TX) function in generally immunosuppressed type 1 diabetic patients (1–5) has led us, after years preclinical study (6), to initiate a phase pilot clinical trial microencapsulated TX into 10 nonimmunosuppressed with diabetes, under permission and surveillance by the Italian Ministry Health (file no. 19382, PRE 805, 5 September 2003). ### Human procurement Human islets were isolated from single-donor pancreases according Edmonton protocol (1)....
This study investigates the effect of dexamethasone on leukocyte extravasation in post-capillary venules hamster cheek pouch, using an intravital microscopy technique, and seeks to clarify potential involvement steroid-inducible protein lipocortin 1. Topical application FMLP (10 nmol), or substance P superfused pouch induced at level three characteristic phenomena rolling, adhesion, transmigration. Pretreatment hamsters with anti-inflammatory dose (1 mg/kg) increased 1 levels circulating...
Type I diabetes mellitus is caused by autoimmune destruction of pancreatic β cells, and effective treatment the disease might require rescuing cell function in a context reinstalled immune tolerance. Sertoli cells (SCs) are found testes, where their main task to provide local immunological protection nourishment developing germ cells. SCs engraft, self-protect, coprotect allogeneic xenogeneic grafts from different experimental settings. have also been successfully implanted into central...
Melatonin (MLT) is a cytoprotective agent holding potential to prevent cadmium (Cd) toxicity and its impact in testicular function fertility. In this study, we explored such porcine pre-pubertal Sertoli cells (SCs). Cd resulted impaired SC viability function, abnormal cellular H2 O2 generation efflux, induction of reductive stress by the upregulation Nrf2 expression activity, cystine uptake glutathione biosynthesis, glutathione-S-transferase P (GSTP) expression, protein glutathionylation...
This study aimed to evaluate the intrinsic in vitro performance of naïve porcine prepubertal Sertoli cells (SCs) and SCs loaded with blank poly(lactic acid) microparticles (MP) or amphotericin B (AmB-MP) against Candida tropicalis, a prevalent pathogenic non-albicans species. The objective was assess their impact on biofilm formation cellular response mechanisms involved, building previous findings that highlight SCs’ potential as anti-infective agents drug carriers. Our results demonstrated...
The description of a microencapsulation procedure for Wharton's jelly mesenchymal stem cells (WJMSCs) is reported. applied method based on the generation monodisperse droplets by vibrational nozzle. An ionic alginate encapsulation was utilized microbeads hardening. Different experimental parameters were analyzed, including frequency and amplitude vibration, polymer pumping rate, distance between nozzle gelling bath. produced barium-alginate characterized excellent morphological...
A method for microencapsulation of isolated neonatal porcine Sertoli cells is described. Using a conventional alginate-poli-l-ornithine encapsulation procedure, which has been used in our laboratory almost two decades to envelop pancreatic islets, we observed significant loss cell viability, possibly due excessive Ca2+ ion exposure. Replacing calcium with barium, or shortening the incubation period presence Ca ions, obtained barium alginate gel microbeads that did not alter morphology and...
The increasing use of nanomaterials in a variety industrial, commercial, medical products, and their environmental spreading has raised concerns regarding potential toxicity on human health. Titanium dioxide nanoparticles (TiO2 NPs) represent one the most commonly used nanoparticles. Emerging evidence suggested that exposure to TiO2 NPs induced reproductive male animals. In this vitro study, porcine prepubertal Sertoli cells (SCs) have undergone acute (24 h) chronic (from 1 up 3 weeks)...
Lately, nickel oxide nanoparticles (NiO NPs) have been employed in different industrial and biomedical fields. Several studies reported that NiO NPs may affect the development of reproductive organs inducing oxidative stress and, resulting male infertility. We investigated vitro effects on porcine pre-pubertal Sertoli cells (SCs) which undergone acute (24 h) chronic (from 1 up to 3 weeks) exposure at two subtoxic doses μg/ml 5 μg/ml. After we performed following analysis: (a) SCs...
The optimization, through a Design of Experiments (DoE) approach, microencapsulation procedure for isolated neonatal porcine islets (NPI) is described. applied method based on the generation monodisperse droplets by vibrational nozzle. An alginate/polyornithine encapsulation procedure, developed and validated in our laboratory almost decade, was used to embody pancreatic islets. We analyzed different experimental parameters including frequency vibration, amplitude polymer pumping rate,...
Pancreatic‐islet‐cell transplantation may reverse hyperglycaemia in diabetic recipients that undertake general pharmacological immunosuppression. A major challenge remains is the need to avoid immunosuppression associated with use of allogeneic or heterologous islet cells. In present study we demonstrate microencapsulation cells using artificial biocompatible and permselective membranes prepared alginic acid derivatives polyamino acids. While characterization microcapsule constituent...
Abstract Background Porcine Sertoli cells ( pSC s) have been employed for cell therapy in pre‐clinical studies several chronic/immune diseases as they deliver molecules associated with trophic and anti‐inflammatory effects. To be human xenografts, s products need to comply safety stability. fulfill such requirements, we a microencapsulation technology increase pre‐transplant storage stability of specific pathogen‐free SPF ‐ evaluated the vivo long‐term viability grafts. Methods Specific...
Abstract Background The nerve growth factor (NGF), a member of the neurotrophins family, plays an important role not only in nervous but also other non-nervous systems such as reproductive system. aim paper is to study vitro effect NGF on rabbit sperm functions. Methods Ten adult bucks were collected five times, and pooled semen samples have been analysed. was quantified seminal plasma, distribution receptors (TrKA p75NTR) established. Moreover, dose-effect motility rate track speed...
Several lines of evidence suggest that insulin-like growth factor 1 (IGF1) is involved in Sertoli cell (SC) proliferation and its receptor (IGF1R) could mediate follicle-stimulating hormone (FSH) effects. To examine the role (GH)-IGF1 axis on SC function, we evaluated effects GH IGF1 basal FSH-modulated proliferation, as well anti-Müllerian (AMH) inhibin B expression secretion in-vitro. SCs from neonatal pigs were incubated with (1) placebo, (2) 100 nM highly purified urofollitropin (hpFSH),...
The Sertoli cells (SeCs) of the seminiferous tubules secrete a multitude immunoregulatory and trophic factors to provide immune protection assist in orderly development germ cells. Grafts naked or encapsulated SeCs have been proved represent an interesting therapeutic option plethora experimental models diseases. However, whether immunosuppressive immunomodulatory effects, which is imperative for their clinical translatability, has not demonstrated. We directly assessed immunopotential...