A5063959885- Bone Metabolism and Diseases
- Mesenchymal stem cell research
- Estrogen and related hormone effects
- Bone health and treatments
- NF-κB Signaling Pathways
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Spine and Intervertebral Disc Pathology
- Osteoarthritis Treatment and Mechanisms
- Bone Tissue Engineering Materials
- Tissue Engineering and Regenerative Medicine
- DNA and Nucleic Acid Chemistry
- Bone and Dental Protein Studies
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- 3D Printing in Biomedical Research
- Electrospun Nanofibers in Biomedical Applications
- Cytokine Signaling Pathways and Interactions
- Dental Implant Techniques and Outcomes
- Periodontal Regeneration and Treatments
- Adenosine and Purinergic Signaling
- Cell Adhesion Molecules Research
- Silk-based biomaterials and applications
- Molecular Biology Techniques and Applications
- Renal and related cancers
University of Ferrara
2016-2025
Albert Einstein College of Medicine
1999
Azienda Ospedaliero-Universitaria di Modena
1999
Azienda Unità Sanitaria Locale di Ferrara
1999
There is a growing demand for the development of experimental strategies efficient articular cartilage repair. Current tissue engineering-based regenerative make use human mesenchymal stromal cells (hMSCs). However, when implanted in defect, control hMSCs differentiation toward chondrogenic lineage remains significant challenge. We have recently demonstrated that silencing antichondrogenic regulator microRNA-221 (miR-221) was highly effective promoting vitro chondrogenesis monolayered...
Abstract Background Osteoclasts (OCs) are involved in rheumatoid arthritis and several pathologies associated with bone loss. Recent results support the concept that some medicinal plants derived natural products of great interest for developing therapeutic strategies against disorders, including osteoporosis. In this study we determined whether extracts Emblica officinalis fruits display activity possible treatment osteoporosis by activating programmed cell death human primary osteoclasts....
The description of a microencapsulation procedure for Wharton's jelly mesenchymal stem cells (WJMSCs) is reported. applied method based on the generation monodisperse droplets by vibrational nozzle. An ionic alginate encapsulation was utilized microbeads hardening. Different experimental parameters were analyzed, including frequency and amplitude vibration, polymer pumping rate, distance between nozzle gelling bath. produced barium-alginate characterized excellent morphological...
Abstract Background Breast cancer and its metastatic progression is mainly directed by epithelial to mesenchymal transition (EMT), a phenomenon supported specific transcription factors miRNAs. Methods In order investigate possible correlation between Slug factor miR-221, we performed gene silencing in MDA-MB-231 breast cells evaluated the expression of genes involved supporting phenotype, using qRT-PCR Western blot analysis. Chromatin immunoprecipitation wound healing assays were employed...
The aim of this study was to investigate the role an antichondrogenic factor, MIR221 (miR-221), in intervertebral disc degeneration (IDD), and provide basic information for development a therapeutic strategy repair based on specific nucleic acid drugs, such as miR-221 silencing.We established relatively quick protocol minimize artifacts from extended vitro culture, without selecting different types cells (IVD) or completely disrupting extracellular matrix (ECM), but by using whole cell...
Regenerative therapies for intervertebral disc (IVD) injuries are currently a major challenge that is addressed in different ways by scientists working this field. Extracellular matrix (ECM) deriving from decellularized non-autologous tissues has been established as biomaterial with remarkable regenerative capacity and its potential therapeutic agent rising. In the present study, we investigated of Wharton's jelly (DWJM) human umbilical cord to act an ECM-based scaffold IVD cell culturing....
Abstract Background Lymphoid Enhancer Factor-1 (Lef-1) is a member of transcription factor family that acts as downstream mediator the Wnt/β-catenin signalling pathway which plays critical role in osteoblast proliferation and differentiation. In search for Lef-1 responsive genes human osteoblasts, we focused on transcriptional regulation SLUG, zinc finger belonging to Snail developmental proteins. Although SLUG epithelial-mesenchymal transition cell motility during embryogenesis well...
The pathways that control mesenchymal stem cells (MSCs) differentiation are not well understood, and although some of the involved transcription factors (TFs) have been characterized, role others remains unclear. We used human MSCs from tibial plateau (TP) trabecular bone, iliac crest (IC) bone marrow Wharton's jelly (WJ) umbilical cord demonstrating a variability in their mineral matrix deposition, expression levels TFs including Runx2, Sox9, Sox5, Sox6, STAT1 Slug, all...
Background/Aims: Mesenchymal stromal cells (MSCs) hold considerable promise in bone tissue engineering, but their poor survival and potency when vivo implanted limits therapeutic potential. For this reason, the study on culture conditions cellular signals that can influence potential outcomes of MSCs have received attention recent years. Cell maintenance under hypoxic conditions, particular for a short period, is beneficial MSCs, as low O2 tension similar to present physiologic niche,...
The development and the optimization of novel culture systems mesenchymal osteoprogenitors are some most important challenges in field bone tissue engineering (TE). A new combination between cells extracellular matrix (ECM)-scaffold, containing ECM has here been analyzed. As source for osteoprogenitors, stem obtained from human umbilical cord Wharton's Jelly (hWJMSCs), were used. ECM-scaffold, a powder form isolated purified porcine urinary bladder (pUBM), was employed. goals current work...
Abstract We have previously demonstrated that collagen type XV (ColXV) is a novel bone extracellular matrix (ECM) protein. It well known the complex mixture of multiple components present in ECM can help both to maintain stemness or promote differentiation stromal cells following change qualitative characteristics concentrations. investigated possible correlation between ColXV expression and mineral deposition by human mesenchymal (hMSCs) with different osteogenic potential osteoblasts...
Bioreactors are devices that efficiently create an environment enables cell cultures to grow in a three-dimensional (3D) context mimicking vivo conditions. In this study, we investigate the effect of dynamic fluid flow on osteogenic potential human mesenchymal stem cells obtained from periodontal ligament and entrapped alginate microbeads.After proper immunophenotyping, were encapsulated barium alginate, cultured 3D static or conditions represented by bioreactor system. Calcein-AM/propidium...
Tumor-specific isoforms generated by alternative splicing (AS) are demonstrated to contribute tumor progression and can represent potential biomarkers. NOVA2 is an AS factor that in physiological conditions regulates endothelial cells' (ECs) polarity vessel lumen maturation, likely mediating of apical-basal regulators. However, expression ECs its regulation have never been investigated.To elucidate this, 40 colorectal cancer patients were enrolled was investigated immunohistochemistry...
Human amniotic fluid mesenchymal stem cells (hAFMSCs) are promising for therapeutic applications in bone damage. Calcium sensing receptor (CaSR), a G protein-coupled receptor, plays physiological role the regulation of metabolism. Thus, CaSR could be targeted by calcimimetic agonists, which may potentially helpful treating diseases. The aim our study was to characterize expression hAFMSCs and assess activity R-568 during vitro osteogenesis. Using western blotting, immunofluorescence, flow...
Abstract The degeneration of intervertebral disc (IVD) is a disease the entire joint between two vertebrae in spine caused by loss extracellular matrix (ECM) integrity, to date with no cure. various regenerative approaches proposed so far have led very limited successes. An emerging opportunity arises from use decellularized ECM as scaffolding material that, directly or combination other materials, has greatly facilitated advancement tissue engineering. Here we focused on obtained human...
The aim of the present study was to determine whether expression estrogen receptor alpha (ERalpha) gene may be a possible target for compounds in plant extracts from Aegle marmelos and Emblica officinalis, used traditional Asian medicine treatment tissue inflammation cancer. To this aim, we evaluated potential selected affect proliferation differentiation ERalpha-negative MDA-MB-231 breast cancer cells, which become ERalpha positive after with decoy molecule against regulatory region human...
Abstract We have used cytokine protein array to analyze the secretion of cytokines from an osteoblastic clone derived human umbilical cord blood mesenchymal stem cells (MSCs) cultured in osteogenic differentiation medium. The analysis demonstrated unexpected ability osteoblast committed and their early progenitors produce significant amounts a range soluble immune mediators without vitro exposure clinically relevant bacterial pathogens. were expanded potential analyzed over 45 days culture...