A5077440082- Neuroscience and Neuropharmacology Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Neurogenesis and neuroplasticity mechanisms
- Distributed and Parallel Computing Systems
- Neural dynamics and brain function
- Methane Hydrates and Related Phenomena
- Meteorological Phenomena and Simulations
- Scientific Computing and Data Management
- Geological Modeling and Analysis
- Advanced Computational Techniques and Applications
- Alzheimer's disease research and treatments
- Receptor Mechanisms and Signaling
- Signaling Pathways in Disease
- Medical Imaging Techniques and Applications
- Zebrafish Biomedical Research Applications
- Nerve injury and regeneration
- Computational Geometry and Mesh Generation
- Climate variability and models
- Geophysics and Gravity Measurements
- Memory and Neural Mechanisms
- Barrier Structure and Function Studies
- Genetics and Neurodevelopmental Disorders
- Immune cells in cancer
- Advanced Memory and Neural Computing
- Adenosine and Purinergic Signaling
University of Illinois Urbana-Champaign
2020-2024
University of California, Los Angeles
2017-2023
Tsinghua University
2006-2023
Czech Academy of Sciences, Institute of Physiology
2021
Shenyang University of Technology
2021
Sichuan University
2020
University of California, Santa Cruz
2009-2017
Astrocytes are ubiquitous in the brain and widely held to be largely identical. However, this view has not been fully tested, possibility that astrocytes neural circuit specialized remains unexplored. Here, we used multiple integrated approaches, including RNA sequencing (RNA-seq), mass spectrometry, electrophysiology, immunohistochemistry, serial block-face-scanning electron microscopy, morphological reconstructions, pharmacogenetics, diffusible dye, calcium, glutamate imaging, directly...
Astrocytes, a type of glia, are abundant and morphologically complex cells. Here, we report astrocyte molecular profiles, diversity, morphology across the mouse central nervous system (CNS). We identified shared region-specific astrocytic genes functions explored cellular origins their regional diversity. gene networks correlated with morphology, several which unexpectedly contained Alzheimer’s disease (AD) risk genes. CRISPR/Cas9–mediated reduction candidate reduced morphological complexity...
Astrocyte gene expression is altered in mouse models of Huntington’s disease and postmortem brain samples from patients with HD.
Abstract Myelin is essential for rapid nerve signaling and increasingly found to play important roles in learning diverse diseases of the CNS. Morphological parameters myelin such as sheath length are thought precisely tune conduction velocity, but mechanisms controlling morphology poorly understood. Local calcium has been observed nascent sheaths can be modulated by neuronal activity. However, role formation remains incompletely Here, we use genetic tools attenuate oligodendrocyte during...
Functional hyperemia occurs when enhanced neuronal activity signals to increase local cerebral blood flow (CBF) satisfy regional energy demand. Ca
The membrane protein Nogo-A is known as an inhibitor of axonal outgrowth and regeneration in the CNS. However, its physiological functions normal adult CNS remain incompletely understood. Here, we investigated role cortical synaptic plasticity motor learning uninjured rodent cortex. receptor NgR1 are present at synapses. Acute treatment slices with function-blocking antibodies (Abs) against or increased long-term potentiation (LTP) induced by stimulation layer 2/3 horizontal fibers....
The mammalian cerebral cortex is typically organized in six layers containing multiple types of neurons, with pyramidal neurons (PNs) being the most abundant. PNs different cortical have distinct morphology, physiology and functional roles neural circuits. Therefore, their development synaptic plasticity may also differ. Using vivo transcranial two-photon microscopy, we followed structural dynamics dendritic spines on apical dendrites layer (L) 2/3 L5 at developmental stages. We show that...
Inflammatory processes induced by brain injury are important for recovery; however, when uncontrolled, inflammation can be deleterious, likely explaining why most anti-inflammatory treatments have failed to improve neurological outcomes after in clinical trials. In the thalamus, chronic activation of glial cells, a proxy inflammation, has been suggested as an indicator increased seizure risk and cognitive deficits that develop cortical injury. Furthermore, lesions more than other regions,...
Astrocytes exist throughout the CNS and affect neural circuits behavior through intracellular Ca 2+ signaling. Studying function(s) of astrocyte signaling has proven difficult because paucity tools to achieve selective attenuation. Based on recent studies, we generated used male female knock-in mice for Cre-dependent expression mCherry-tagged hPMCA2w/b attenuate in genetically defined cells vivo (CalEx flox lcium Ex trusion). We characterized CalEx following local AAV-Cre microinjections...
SUMMARY Microglial calcium signaling is rare in a baseline state but shows strong engagement during early epilepsy development. The mechanism and purpose behind microglial not known. By developing an vivo UDP fluorescent sensor, GRAB UDP1.0 , we discovered that release conserved response to seizures excitotoxicity across brain regions. signals the P2Y 6 receptor for broad increases epileptogenesis. UDP-P2Y necessary lysosome upregulation limbic regions enhances production of pro-inflammatory...
Abstract Tropical cyclones (TCs), especially landfalling intense storms often pose serious threats to life and property in coastal areas. Although TC track forecast skill has been improved the past decades, progress of advancing intensity lags that forecast. One possible limiting factor is absence ocean surface waves systems. To better represent interaction underlying ocean, a regional atmosphere‐ocean‐wave coupled model employed this study. Twenty‐one TCs whole year 2013 are retrospectively...
In the mammalian cortex, neurons form extremely complicated networks and exchange information at synapses. Changes in synaptic strength, as well addition/removal of synapses, occur an experience-dependent manner, providing structural foundation neuronal plasticity. As postsynaptic components most excitatory synapses dendritic spines are considered to be a good proxy Taking advantages mouse genetics fluorescent labeling techniques, individual their structures can labeled intact brain. Here we...
The effects of beta amyloid (Abeta) on cytoplasmic Ca(2+) ([Ca(2+)](c)) have been studied extensively, but the current literature this aspect is confusing. We reported that 20 microM Abeta(25-35) significantly inhibited synchronized spontaneous transients immediately after application, whereas it had little effect baseline [Ca(2+)](c) concentration in neurons. Abeta(1-42) a similar as Abeta(25-35), while increased gradually. However, Abeta(1-40) either or [Ca(2+)](c). Such differential Abeta...