A5023975863- Gut microbiota and health
- Metabolomics and Mass Spectrometry Studies
- Diet and metabolism studies
- Gene expression and cancer classification
- Rheumatoid Arthritis Research and Therapies
- Bioinformatics and Genomic Networks
- Traditional Chinese Medicine Studies
- Clostridium difficile and Clostridium perfringens research
- Helicobacter pylori-related gastroenterology studies
- Colorectal Cancer Screening and Detection
- Microbial Metabolic Engineering and Bioproduction
- Bone health and treatments
- Gastric Cancer Management and Outcomes
- Food Security and Health in Diverse Populations
- Nutritional Studies and Diet
- Pediatric Hepatobiliary Diseases and Treatments
- Radiopharmaceutical Chemistry and Applications
- Liver Diseases and Immunity
- Genetic factors in colorectal cancer
- Molecular Biology Techniques and Applications
- Advanced Breast Cancer Therapies
- Liver Disease Diagnosis and Treatment
- Microbial Natural Products and Biosynthesis
- Bone health and osteoporosis research
- Breast Cancer Treatment Studies
Mayo Clinic
2018-2025
Mayo Clinic in Arizona
2019-2025
University of Minnesota Rochester
2024
WinnMed
2019-2024
Mayo Clinic in Florida
2019-2024
University of Minnesota
2022
Nemours Children’s Clinic
2022
Jacksonville College
2019
Harvard University
2018
Broad Institute
2018
Abstract A system-level framework of complex microbe–microbe and host–microbe chemical cross-talk would help elucidate the role our gut microbiota in health disease. Here we report a literature-curated interspecies network human microbiota, called NJS16. This is an extensive data resource composed ∼570 microbial species 3 cell types metabolically interacting through >4,400 small-molecule transport macromolecule degradation events. Based on contents network, develop mathematical approach...
Abstract Providing insight into one’s health status from a gut microbiome sample is an important clinical goal in current human research. Herein, we introduce the Gut Microbiome Health Index (GMHI), biologically-interpretable mathematical formula for predicting likelihood of disease independent diagnosis. GMHI formulated upon 50 microbial species associated with healthy ecosystems. These are identified through multi-study, integrative analysis on 4347 stool metagenomes 34 published studies...
Colorectal adenomas are precursors of CRC. Recently, the gut microbiota, i.e., collection microbes residing in our gut, has been recognized as a key player CRC development. There have number microbiota profiling studies for colorectal adenoma and CRC; however, fewer considered metabolome, which serves chemical interface between host microbiota. Here, we conducted metabolome study analyzed metabolomic profiles together with paired composition profiles. We found several signatures that were...
Links between colorectal cancer (CRC) and the gut microbiome have been established, but specific microbial species their role in carcinogenesis remain an active area of inquiry. Our understanding would be enhanced by better accounting for tumor subtype, community interactions, metabolism, ecology.
Abstract Incidence rates for endometrial cancer (EC) are rising, particularly in postmenopausal and obese women. Previously, we showed that the uterine vaginal microbiome distinguishes patients with EC from those without. Here, sought to examine impact of patient factors (such as menopause status, body mass index, pH) absence how these might contribute signature EC. We find each factor independently alters identified status main driver a polymicrobial network associated (ECbiome)....
Abstract Background Rapid advances in the past decade have shown that dysbiosis of gut microbiome is a key hallmark rheumatoid arthritis (RA). Yet, relationship between and clinical improvement RA disease activity remains unclear. In this study, we explored patients with to identify features are associated with, as well predictive of, minimum clinically important (MCII) activity. Methods We conducted retrospective, observational cohort study on diagnosed 1988 2014. Whole metagenome shotgun...
Recent advancements in translational gut microbiome research have revealed its crucial role shaping predictive healthcare applications. Herein, we introduce the Gut Microbiome Wellness Index 2 (GMWI2), an enhanced version of our original GMWI prototype, designed as a standardized disease-agnostic health status indicator based on taxonomic profiles. Our analysis involves pooling existing 8069 stool shotgun metagenomes from 54 published studies across global demographic landscape (spanning 26...
ABSTRACT Bacterial persisters are a small fraction of quiescent cells that survive in the presence lethal concentrations antibiotics. They can regrow to give rise new population has same vulnerability antibiotics as did parental population. Although formation bacterial various been documented, molecular mechanisms by which these tolerate still controversial. We found amplification fumarate reductase operon ( FRD ) Escherichia coli led higher frequency persister formation. The E. was...
To date, there has been no multi-omic analysis characterizing the intricate relationships between intragastric microbiome and gastric mucosal gene expression in carcinogenesis. Using approaches, we provide a comprehensive view of connections host distinct stages carcinogenesis (i.e., healthy, gastritis, cancer). Our integrative uncovers various associations specific to disease states. For example, uniquely Helicobacteraceae is highly correlated with FAM3D, which previously implicated...
Abstract Background Dysbiosis of the gut microbiome is frequent in intensive care unit (ICU), potentially leading to a heightened risk nosocomial infections. Enhancing has been proposed as strategic approach mitigate potential adverse outcomes. While prior research on select probiotic supplements not successfully shown improve microbial diversity, fermented foods offer promising alternative. In this open-label phase I safety and feasibility study, we examined kefir an initial step towards...
Spinal cord injury (SCI) significantly alters gene expression, potentially impeding functional recovery. This study investigated the effects of atorvastatin, a widely prescribed cholesterol-lowering drug, on expression and recovery in chronic murine SCI model. Female C57BL/6J mice underwent moderate 0.25 mm lateral compression received daily atorvastatin (10 mg/kg) or vehicle-only injections from two weeks post-injury for four weeks. Sensorimotor functions were assessed using Basso Mouse...
Gut microbial symbionts have been shown to influence the development of autoimmunity in multiple sclerosis (MS). Emerging research points an important relationship between microbial-IgA interface and MS pathophysiology. IgA-secreting B cells are observed brain, shifts gut bacteria-IgA binding described some patients with MS. However, relationships microbiome host immune response, particularly regarding B-cell-depleting immunomodulation, remain underexplored. This study aimed evaluate...
BACKGROUND: Pharmacological treatments for fibrocalcific aortic valve stenosis (FCAVS) have been elusive >50 years. Here, we tested the hypothesis that reactivation of oxidized sGC (soluble guanylate cyclase), primary receptor nitric oxide, with ataciguat is a safe and efficacious strategy to slow progression FCAVS. METHODS: We used quantitative real-time reverse transcription polymerase chain reaction, Western blotting, immunohistochemistry characterize signaling biological effects on...
Abstract Background Rheumatoid arthritis (RA) is a chronic, autoimmune disorder characterized by joint inflammation and pain. In patients with RA, metabolomic approaches, i.e., high-throughput profiling of small-molecule metabolites, on plasma or serum has thus far enabled the discovery biomarkers for clinical subgroups, risk factors, predictors treatment response. Despite these recent advancements, identification blood metabolites that reflect quantitative disease activity remains an...
Chemotherapy-induced cognitive impairment (CICI) has emerged as a significant medical problem without therapeutic options. Using the platinum-based chemotherapy cisplatin to model CICI, we revealed robust elevations in adenosine A 2A receptor (A R) and its downstream effectors, cAMP CREB, by adult mouse hippocampus, critical brain structure for learning memory. Notably, R inhibition Food Drug Administration–approved antagonist KW-6002 prevented cisplatin-induced impairments neural progenitor...
Abstract We previously proposed the Gut Microbiome Wellness Index (GMWI), a predictor of disease presence based on gut microbiome taxonomic profile. As an application this index for food science research, we applied GMWI as quantitative tool measuring prebiotic effect oligosaccharides. Mainly, in vitro anaerobic batch fermentation system, fructooligosaccharides (FOS), galactooligosaccharides (GOS), xylooligosaccharides (XOS), inulin (IN), and 2’-fucosyllactose (2FL), were mixed separately...
Primary sclerosing cholangitis (PSC) is a pathogenically complex, chronic, fibroinflammatory disorder of the bile ducts without known etiology or effective pharmacotherapy. Emerging in vitro and vivo evidence support fundamental pathophysiologic mechanisms PSC centered on enterohepatic circulation. To date, no studies have specifically interrogated chemical footprint circulation PSC. Herein, we evaluated metabolome lipidome portal venous blood obtained at time liver transplantation patients...
SUMMARY Microglial calcium signaling is rare in a baseline state but shows strong engagement during early epilepsy development. The mechanism and purpose behind microglial not known. By developing an vivo UDP fluorescent sensor, GRAB UDP1.0 , we discovered that release conserved response to seizures excitotoxicity across brain regions. signals the P2Y 6 receptor for broad increases epileptogenesis. UDP-P2Y necessary lysosome upregulation limbic regions enhances production of pro-inflammatory...