A5078154869- Neuroinflammation and Neurodegeneration Mechanisms
- Immune Response and Inflammation
- Immune cells in cancer
- Aquaculture disease management and microbiota
- interferon and immune responses
- Neuroscience and Neuropharmacology Research
- Luminescence Properties of Advanced Materials
- Pain Mechanisms and Treatments
- Viral Infections and Vectors
- Cancer-related molecular mechanisms research
- Plasmonic and Surface Plasmon Research
- Spinal Cord Injury Research
- Immune Cell Function and Interaction
- Malaria Research and Control
- Adenosine and Purinergic Signaling
- Solid State Laser Technologies
- Chemokine receptors and signaling
- Animal Virus Infections Studies
- Pharmacological Effects of Natural Compounds
- Inflammation biomarkers and pathways
- Immunotherapy and Immune Responses
- Photonic and Optical Devices
- Alzheimer's disease research and treatments
- Advanced Biosensing Techniques and Applications
- T-cell and B-cell Immunology
Mayo Clinic
2018-2025
WinnMed
2020-2025
The University of Texas Health Science Center at Houston
2024-2025
Sixth Affiliated Hospital of Sun Yat-sen University
2025
Sun Yat-sen University
2019-2025
Shenyang Pharmaceutical University
2025
Capital Medical University
2025
South China Agricultural University
2018-2024
Mayo Clinic in Arizona
2019-2024
Fujian Normal University
2024
The apolipoprotein E (
Gliosis is a histopathological characteristic of epilepsy that comprises activated microglia and astrocytes. It unclear whether or how crosstalk occurs between astrocytes in the evolution epilepsy. Here, we report mouse model status epilepticus, induced by intracerebroventricular injection kainic acid (KA), sequential activation their close spatial interaction hippocampal CA3 region. Microglial ablation reduced astrocyte upregulation complement C3. When compared to wild-type mice, both C3
TREM2 is exclusively expressed by microglia in the brain and strongly linked to risk for Alzheimer’s disease (AD). As microglial responses modulated are central AD pathogenesis, enhancing signaling has been explored as an therapeutic strategy. However, effective window targeting unclear. Here, using microglia-specific inducible mouse models overexpressing human wild-type (TREM2-WT) or R47H variant (TREM2-R47H), we show that TREM2-WT expression reduces amyloid deposition neuritic dystrophy...
Optical fibers can be effective biosensors when employed in early-stage diagnostic point-of-care devices as they avoid interference from molecules with similar redox potentials. Nevertheless, their sensitivity needs to improved for real-world applications, especially small-molecule detection. This work demonstrates an optical microfiber biosensor dopamine (DA) detection based on the DA-binding-induced aptamer conformational transitions that occur at plasmonic coupling sites a...
Abstract Immunosuppression of unknown aetiology is a hallmark feature glioblastoma and characterized by decreased CD4 T-cell counts downregulation major histocompatibility complex class II expression on peripheral blood monocytes in patients. This immunosuppression critical barrier to the successful development immunotherapies for glioblastoma. We recapitulated observed patients C57BL/6 mouse investigated low counts. determined that thymic involution was three distinct models brain cancer,...
Spinal microglia are highly responsive to peripheral nerve injury and known be a key player in pain. However, there has not been direct evidence showing that selective microglial activation vivo is sufficient induce chronic Here, we used optogenetic approaches address this question employing CX3CR1 creER/+ : R26 LSL-ReaChR/+ transgenic mice, which red-activated channelrhodopsin (ReaChR) inducibly specifically expressed microglia. We found of ReaChR by red light spinal evoked reliable inward...
Abstract Microglia are known to engage in physical interactions with neurons. However, our understanding of the detailed mechanistic regulation microglia-neuron is incomplete. Here, using high resolution two photon imaging, we investigated NMDA receptor-induced interactions. We found that GluN2A inhibitor NVPAAM007, but not GluN2B ifenprodil, blocked occurrence these Consistent well-known developmental subunit, absent neonatal tissues. Furthermore, consistent a preferential synaptic...
Microglia have been shown to be of critical importance the progression temporal lobe epilepsy. However, broad transcriptional changes that these cells undergo following seizure induction is not well understood. As such, we utilized RNAseq analysis upon microglia isolated from hippocampus determine expression pattern alterations kainic acid induced seizure. We determined dramatic their patterns, particularly with regard mitochondrial activity and metabolism. also observed initiate...
Border-associated macrophages (BAMs) are tissue-resident that reside at the border of central nervous system (CNS). Since BAMs originate from yolk sac progenitors do not persist after birth, means by which this population cells is maintained well understood. Using two-photon microscopy and multiple lineage-tracing strategies, we determine CCR2
Abstract Motor cortical hyperexcitability is well-documented in the presymptomatic stage of amyotrophic lateral sclerosis (ALS). However, mechanisms underlying this early dysregulation are not fully understood. Microglia, as principal immune cells central nervous system, have emerged important players sensing and regulating neuronal activity. Here we investigated role microglia motor circuits a mouse model TDP-43 neurodegeneration (rNLS8). Utilizing multichannel probe recording longitudinal...
Abstract Activation of spinal cord microglia contributes to the development peripheral nerve injury-induced neuropathic pain. However, molecular mechanisms underlying microglial function in pain are not fully understood. We identified that voltage-gated proton channel Hv1, which is functionally expressed microglia, was significantly increased after transection (SNT). Hv1 mediated currents and mice lacking (Hv1 KO) display attenuated hypersensitivities SNT compared with wildtype (WT) mice. In...
Myeloid cells comprise up to 50% of the total tumor mass in glioblastoma (GBM) and have been implicated promoting progression immunosuppression. Modulating response myeloid has emerged as a promising new approach for cancer treatment. In this regard, we focus on Triggering Receptor Expressed Cells 2 (TREM2), which recently novel immune modulator peripheral tumors.
Abstract Activation of innate immunity in the brain is a prominent feature Alzheimer’s disease (AD). The present study investigated regulation by wild-type serum injection transgenic AD mouse model. We found that treatment with significantly reduced number neutrophils and microglial reactivity brains APP/PS1 mice. Mimicking this effect, neutrophil depletion via Ly6G neutralizing antibodies resulted improvements functions. Serum proteomic analysis identified vascular endothelial growth...
Microglia actively survey the brain and dynamically interact with neurons to maintain homeostasis. Microglial Gi protein–coupled receptors (Gi-GPCRs) play a critical role in microglia-neuron communications. However, impact of temporally activating microglial signaling on dynamics neuronal activity homeostatic remains largely unknown. In this study, we used Gi-based designer exclusively activated by drugs (Gi-DREADD) selectively modulate pathway. By integrating chemogenetic approach vivo...