A5021586993- Pesticide Exposure and Toxicity
- Parkinson's Disease Mechanisms and Treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Neuroscience and Neuropharmacology Research
- Alzheimer's disease research and treatments
- Cholinesterase and Neurodegenerative Diseases
- Marine Biology and Ecology Research
- Effects and risks of endocrine disrupting chemicals
- Insect and Pesticide Research
- Nuclear Receptors and Signaling
- Nerve injury and regeneration
- Trace Elements in Health
- Neurological disorders and treatments
- Environmental Toxicology and Ecotoxicology
- Drug Transport and Resistance Mechanisms
- Adenosine and Purinergic Signaling
- Pesticide and Herbicide Environmental Studies
- Pharmacological Effects and Toxicity Studies
- Neurotransmitter Receptor Influence on Behavior
- Anesthesia and Neurotoxicity Research
- Pharmacogenetics and Drug Metabolism
- Epigenetics and DNA Methylation
- Histone Deacetylase Inhibitors Research
- Prenatal Substance Exposure Effects
- Marine and coastal plant biology
Florida International University
2014-2024
University of Georgia
2024
Environmental and Occupational Health Sciences Institute
2014-2023
Rutgers, The State University of New Jersey
2014-2023
Northeast Ohio Medical University
2014-2023
University of California, Riverside
2020
Johnson University
2011-2015
Nottingham University Hospitals NHS Trust
2014
New York Medical College
2014
Lehigh University
2014
Exposure of rats to the pesticide and complex I inhibitor rotenone reproduces features Parkinson's disease, including selective nigrostriatal dopaminergic degeneration alpha-synuclein-positive cytoplasmic inclusions (Betarbet et al., 2000; Sherer 2003). Here, we examined mechanisms toxicity using three model systems. In SK-N-MC human neuroblastoma cells, (10 nm 1 microm) caused dose-dependent ATP depletion, oxidative damage, death. To determine molecular site action rotenone, cells were...
The vesicular monoamine transporter 2 (VMAT2; SLC18A2) is responsible for packaging dopamine into vesicles subsequent release and has been suggested to serve a neuroprotective role in the system. Here, we show that mice express approximately 5% of normal VMAT2 (VMAT2 LO) display age-associated nigrostriatal dysfunction ultimately results neurodegeneration. Elevated cysteinyl adducts L-DOPA DOPAC are seen early followed by increased striatal protein carbonyl 3-nitrotyrosine formation. These...
Abstract Parkinson’s disease (PD) has been linked to mitochondrial dysfunction and pesticide exposure. The rotenone (ROT) inhibits complex I reproduces features of PD in animal models, suggesting that environmental agents inhibit may contribute PD. We have previously demonstrated ROT toxicity is dependent upon inhibition oxidative stress the primary mechanism toxicity. In this study, we examined vitro action several putative inhibitors are commonly used as pesticides. rank order pesticides...
<h3>Importance</h3> The causes of late-onset Alzheimer disease (AD) are not yet understood but likely include a combination genetic, environmental, and lifestyle factors. Limited epidemiological studies suggest that occupational pesticide exposures associated with AD. Previously, we reported serum levels dichlorodiphenyldichloroethylene (DDE), the metabolite dichlorodiphenyltrichloroethane (DDT), were elevated in small number patients AD (n=20). <h3>Objective</h3> To evaluate association...
Spinal long-term potentiation (LTP) at C-fiber synapses is hypothesized to underlie chronic pain. However, a causal link between spinal LTP and pain still lacking. Here, we report that high-frequency stimulation (HFS; 100 Hz, 10 V) of the mouse sciatic nerve reliably induces without causing injury. LTP-inducible triggers lasting for more than 35 days increases number calcitonin gene-related peptide (CGRP) terminals in dorsal horn. The behavioral morphological changes can be prevented by...
Paraquat, MPTP, and rotenone reproduce features of Parkinson's disease (PD) in experimental animals. The exact mechanisms by which these compounds damage the dopamine system are not firmly established, but selective to neurons inhibition complex I thought be involved. We others have previously documented that toxic metabolite MPP+, is transported into through transporter (DAT), while DAT. also demonstrated requirement for oxidative dopaminergic neurodegeneration produced rotenone. Based on...
Exposure to pesticides has been suggested increase the risk of Parkinson's disease (PD), but mechanisms responsible for this association are not clear. Here, we report that perinatal exposure mice during gestation and lactation low levels dieldrin (0.3, 1, or 3 mg/kg every days) alters dopaminergic neurochemistry in their offspring exacerbates MPTP toxicity. At 12 wk age, protein mRNA dopamine transporter (DAT) vesicular monoamine 2 (VMAT2) were increased by a dose-related manner. We then...
Pyrethroid insecticides are one of the most commonly used residential and agricultural insecticides.Based on increased use pyrethroids recent studies showing that pregnant women children exposed to pyrethroids, there concerns over potential for developmental neurotoxicity.However, have been relatively few neurotoxicity pyrethroids.In this study, we sought investigate toxicity six common three type I compounds (permethrin, resmethrin, bifenthrin) II (deltamethrin, cypermethrin,...
Neuroinflammation is one of the hallmarks neurodegenerative diseases, such as Parkinson's disease (PD). Activation glial cells, including microglia and astrocytes, a characteristic inflammatory response. Glycoprotein non-metastatic melanoma protein B (GPNMB) transmembrane glycoprotein that releases soluble signaling peptide when cleaved by ADAM10 or other extracellular proteases. GPNMB has demonstrated neuroprotective role in animal models ALS ischemia. However, mechanism this protection not...
Pyrethroid pesticides cause abnormalities in the dopamine system and produce an ADHD phenotype animal models, with effects accentuated males versus females. However, data regarding behavioral of pyrethroid exposure children is limited. We examined association between pesticide a nationally representative sample US children, tested whether this differs by sex. Data are from 8–15 year old participants (N = 687) 2001–2002 National Health Nutrition Examination Survey. Exposure was assessed using...
Exposure to the pyrethroid pesticide deltamethrin has been demonstrated cause apoptosis both in vitro and vivo. However, molecular pathways leading deltamethrin-induced have not established. To identify these pathways, SK-N-AS neuroblastoma cells were exposed (100nM–5μM) for 24–48 h. Deltamethrin produced a time- dose-dependent increase (21–300%) DNA fragmentation, an indicator of apoptosis. Data demonstrate that initiation fragmentation resulted from interaction with Na+ channels consequent...
Attention-deficit hyperactivity disorder (ADHD) is estimated to affect 8-12% of school-age children worldwide. ADHD a complex with significant genetic contributions. However, no single gene has been linked percentage cases, suggesting that environmental factors may contribute ADHD. Here, we used behavioral, molecular, and neurochemical techniques characterize the effects developmental exposure pyrethroid pesticide deltamethrin. We also epidemiologic methods determine whether there an...
The P2Y12 receptor (P2Y12R) is a purinoceptor that selectively expressed in microglia the central nervous system. As signature receptor, microglial P2Y12R mediates process chemotaxis towards ADP/ATP gradients and engaged several neurological diseases including chronic pain, stroke seizures. However, role of regulating neuronal excitability innate behaviors not fully understood. Here, we generated P2Y12-floxed mice to delete beginning development (CX3CR1Cre/+:P2Y12f/f; "constitutive...
Extracellular calcium concentrations in the brain fluctuate during neuronal activities and may affect behavior of cells. Microglia are highly dynamic immune cells brain. However, effects extracellular on microglial dynamics have not been investigated. Here, we addressed this question mouse slices vivo using two-photon microscopy. We serendipitously found that reduction induced processes to converge at distinct sites, a phenomenon termed process convergence (MPCs). Our studies revealed MPCs...
Microglia are the resident immune cells of center nervous system and participate in various neurological diseases. Here we determined function microglia epileptogenesis using microglial ablation approaches. Three different microglia-specific genetic tools were used, CX3CR1
Fibroblast growth factor (FGF) 15 (human homolog, FGF19) is an endocrine FGF highly expressed in the small intestine of mice. Emerging evidence suggests that FGF15 critical for regulating hepatic functions; however, role liver regeneration unclear. This study assessed whether altered knockout (KO) mice following 2/3 partial hepatectomy (PHx). The results showed KO had marked mortality, with survival rate influenced by genetic background. Compared wild-type mice, displayed extensive necrosis...
Abstract Increased pro‐inflammatory cytokine levels and proliferation of activated microglia have been found in Parkinson's disease (PD) patients animal models PD, suggesting that targeting the microglial inflammatory response may result neuroprotection PD. Microglial is regulated by many factors, but colony stimulating factor‐1 receptor (CSF1R) has emerged as a primary factor. Using data mining techniques on existing microarray data, we mRNA expression CSF1R ligand, CSF‐1, increased brain...
Abstract Microglial activation has been recognized as a major contributor to inflammation of the epileptic brain. Seizures are commonly accompanied by remarkable microgliosis and loss neurons. In this study, we utilize CX3CR1 GFP/+ CCR2 RFP/+ genetic mouse model, in which + resident microglia monocytes labeled with GFP RFP, respectively. Using combination time‐lapse two‐photon imaging whole‐cell patch clamp recording, determined distinct morphological, dynamic, electrophysiological...
Paraquat, N-methyl-4-phenyl-1,2,3,6 tetrahydropyridine, and rotenone have been shown to reproduce several features of Parkinson's disease in animal cell culture models. Although these chemicals are known perturb dopamine homeostasis induce dopaminergic death, their molecular mechanisms action not well defined. We previously that paraquat does require functional transporter inhibit mitochondrial complex I order mediate its toxic (Richardson et al., 2005). In this study, we show specifically...