A5047303618- Endoplasmic Reticulum Stress and Disease
- Autophagy in Disease and Therapy
- Ubiquitin and proteasome pathways
- Circadian rhythm and melatonin
- Nanoplatforms for cancer theranostics
- Nanoparticle-Based Drug Delivery
- RNA modifications and cancer
- MicroRNA in disease regulation
- RNA regulation and disease
- Angiogenesis and VEGF in Cancer
- Nerve injury and regeneration
- Phagocytosis and Immune Regulation
- Adenosine and Purinergic Signaling
- Neuroinflammation and Neurodegeneration Mechanisms
- Liver physiology and pathology
- Immune cells in cancer
- Tryptophan and brain disorders
- Protease and Inhibitor Mechanisms
- Cancer-related gene regulation
- Anesthesia and Neurotoxicity Research
- Pancreatic function and diabetes
- Alzheimer's disease research and treatments
- Mitochondrial Function and Pathology
- Advanced biosensing and bioanalysis techniques
- Liver Disease Diagnosis and Treatment
Anhui Medical University
2016-2025
First Affiliated Hospital of Anhui Medical University
2008-2025
Zhejiang Wanli University
2025
Anhui Provincial Hospital
2025
The Seventh Affiliated Hospital of Sun Yat-sen University
2021-2022
Sun Yat-sen University
2021-2022
First People's Hospital of Yunnan Province
2020
Dalian Medical University
2020
Chongqing Medical University
2020
Second Affiliated Hospital of Chongqing Medical University
2020
Endoplasmic reticulum-associated degradation (ERAD) is a protein quality control mechanism that eliminates unwanted proteins from the endoplasmic reticulum (ER) through ubiquitin-dependent proteasomal pathway. gp78 previously described ER membrane-anchored ubiquitin ligase (E3) involved in ubiquitination of proteins. AAA ATPase (ATPase associated with various cellular activities) p97/valosin-containing (VCP) subsequently dislodges ubiquitinated and chaperones them to cytosol, where they...
Background and Aims Endoplasmic reticulum (ER) stress is associated with liver inflammation hepatocellular carcinoma (HCC). However, how ER links HCC remains obscure. Mesencephalic astrocyte‐derived neurotrophic factor (MANF) an stress‐inducible secretion protein that inhibits by interacting the key subunit of nuclear kappa light chain enhancer activated B cells (NF‐κB) p65. We hypothesized MANF may play a role in linking HCC. Approach Results Here, we found mRNA levels were lower tissues...
Abstract Inflammation can cause endoplasmic reticulum (ER) stress and therefore activates the unfolded protein response (UPR). ER consequent UPR have potential to activate NF-κB. However, factors mediating crosstalk between NF-κB pathway remain unclear. Here, we determined that inducible Mesencephalic Astrocyte-derived Neurotrophic Factor (MANF) was up-regulated in autoimmune diseases inflammatory disease models. caused MANF relocalize nuclei. interacted with DNA binding domain of p65...
Abstract Microglial activation has been recognized as a major contributor to inflammation of the epileptic brain. Seizures are commonly accompanied by remarkable microgliosis and loss neurons. In this study, we utilize CX3CR1 GFP/+ CCR2 RFP/+ genetic mouse model, in which + resident microglia monocytes labeled with GFP RFP, respectively. Using combination time‐lapse two‐photon imaging whole‐cell patch clamp recording, determined distinct morphological, dynamic, electrophysiological...
Extracellular accumulation of amyloid β-peptide (Aβ) is one pathological hallmarks Alzheimer's disease (AD) and contributes to the neuronal loss. Mesencephalic astrocyte-derived neurotrophic factor (MANF) an endoplasmic reticulum (ER) stress-inducible factor. Many groups, including ours, have proved that MANF rescues loss in several neurological disorders, such as Parkinson's cerebral ischemia. However, whether exerts its protective effect against Aβ neurotoxicity AD remains unknown.In...
Mesencephalic astrocyte-derived neurotrophic factor (MANF), an endoplasmic reticulum stress-inducible secreting protein, has evolutionarily conserved immune-regulatory function that contributes to the negative regulation of inflammation in macrophages. In this study, we investigated profiles MANF macrophages patients with active inflammatory bowel disease (IBD) and mice experimental colitis, which was induced both myeloid cell-specific knockout wild-type by 3% dextran sodium sulfate (DSS)...
Abstract Colorectal cancer (CRC) usually creates an immunosuppressive microenvironment, thereby hindering immunotherapy response. Effective treatment options remain elusive. Using scRNA‐seq analysis in a tumor‐bearing murine model, it is found that lobeline, alkaloid from the herbal medicine lobelia, promotes polarization of tumor‐associated macrophages (TAMs) toward M1‐like TAMs while inhibiting their M2‐like TAMs. Additionally, lobeline upregulates mRNA expression secreted...
Improperly folded proteins in the endoplasmic reticulum (ER) are eliminated via ER-associated degradation, a process that dislocates misfolded from ER membrane into cytosol, where they undergo proteasomal degradation. Dislocation requires subclass of ubiquitin ligases includes gp78 addition to AAA ATPase p97/VCP and its cofactor, Ufd1-Npl4 dimer. We have previously reported interacts directly with p97/VCP. Here, we identify novel p97/VCP-interacting motif (VIM) within mediates this...
Cerebral ischemia-induced accumulation of unfolded proteins in vulnerable neurons triggers endoplasmic reticulum (ER) stress. Arginine-rich, mutated early stage tumors (ARMET) is an ER stress-inducible protein and upregulated the cerebral ischemia. The purposes this study were to investigate characteristics implications ARMET expression induced by focal Focal ischemia rats was right middle artery occlusion with a suture; ischemic lesions assessed magnetic resonance imaging histology;...
Colorectal cancer (CRC), a malignant tumor worldwide consists of microsatellite instability (MSI) and stable (MSS) phenotypes. Although SHP2 is hopeful target for therapy, its relationship with innate immunosuppression remains elusive. To address that, single-cell RNA sequencing was performed to explore the role in all cell types microenvironment (TME) from murine MC38 xenografts. Intratumoral cells were found be functionally heterogeneous responded significantly SHP099, allosteric...
The mesencephalic astrocyte-derived neurotrophic factor (MANF) has been recently identified as a factor, but its role in hepatic fibrosis is unknown. Here, we found that MANF was upregulated the fibrotic liver tissues of patients with chronic diseases and mice treated CCl4. deficiency either hepatocytes or mono-macrophages, particularly clearly exacerbated fibrosis. Myeloid-specific knockout increased population Ly6Chigh macrophages promoted HSCs activation. Furthermore, MANF-sufficient...
Peroxiredoxin 6 (Prdx6), a unique 1-Cys member of the peroxiredoxin family, exhibits peroxidase activity, phospholipase and lysophosphatidylcholine acyltransferase (LPCAT) activity. Prdx6 has been known to be an important enzyme for maintenance lipid peroxidation repair, cellular metabolism, inflammatory signaling, antioxidant damage. Growing research demonstrated that altered activity this is linked with various pathological processes including central nervous system (CNS) disorders. This...
To investigate the role of melatonin in D-galactose-induced amnesic mice, avoidance/escape and water maze tests were performed to evaluate their learning memory function. Spectrophotometry was employed determine content thiobarbituric acid-reactive substances (TBARS) activities antioxidative enzymes brain. The present results demonstrate that mice had significantly decreased reduced superoxide dismutase glutathione peroxidase increased levels TBARS found brain tissue mice. Melatonin,...
Misfolded proteins in the endoplasmic reticulum (ER) are eliminated by a process known as ER-associated degradation (ERAD), which starts with misfolded protein recognition, followed ubiquitination, retrotranslocation to cytosol, deglycosylation, and targeting proteasome for degradation. Actions of multisubunit machineries ER membrane integrate these steps. We hypothesized that regulation machinery assembly is mechanism ERAD activity regulated. To test this hypothesis, we investigated...
Mesencephalic astrocyte-derived neurotrophic factor (MANF), a 20 kDa secreted protein, was originally derived from rat mesencephalic type-1 astrocyte cell line. MANF belongs to novel evolutionally conserved family of factors along with dopamine factor. In recent years, ever-increasing evidence has shown that both them play remarkable protective role against various injuries neurons in vivo or vitro. However, the characteristics expression different types glial cells, especially astrocytes,...
SERPINA1/AAT/α-1-antitrypsin (serpin family A member 1) deficiency (SERPINA1/ AAT-D) is an autosomal recessive disorder characterized by the retention of misfolded SERPINA1/AAT in endoplasmic reticulum (ER) hepatocytes and a significant reduction serum level. The Z variant SERPINA1/AAT, containing Glu342Lys (E342K) mutation (SERPINA1E342K/ATZ), most common form SERPINA1/AAT-D, prone to misfolding polymerization, which retains it ER leads liver injury. Both proteasome macroautophagy/autophagy...
Abstract: Melatonin‐selenium nanoparticles (MT‐Se), a novel complex, were synthesized by preparing selenium in melatonin medium. The present investigation was designed to determine the protective effects of MT‐Se against Bacillus Calmette–Guérin (BCG)/lipopolysaccharide (LPS)‐induced hepatic injury mice. In BCG/LPS‐induced model, administered (i.g.) at doses 5, 10, or 20 mg/kg BCG/LPS‐treated mice for 10 days, significantly reduced increase plasma aminotransferase, severe extent cell damage...