A5101857886- Gut microbiota and health
- MicroRNA in disease regulation
- Probiotics and Fermented Foods
- Cancer-related molecular mechanisms research
- Rice Cultivation and Yield Improvement
- Biofuel production and bioconversion
- RNA Research and Splicing
- Genetic Mapping and Diversity in Plants and Animals
- Cancer Immunotherapy and Biomarkers
- Fungal and yeast genetics research
- Microbial Metabolic Engineering and Bioproduction
- RNA modifications and cancer
- Immunotherapy and Immune Responses
- Barrier Structure and Function Studies
- Agriculture, Soil, Plant Science
- Ferroptosis and cancer prognosis
- Plant Disease Resistance and Genetics
- CAR-T cell therapy research
- GABA and Rice Research
- Circular RNAs in diseases
- Advanced biosensing and bioanalysis techniques
- Clostridium difficile and Clostridium perfringens research
- Plant Molecular Biology Research
- Virus-based gene therapy research
- Cancer Research and Treatments
Shanghai Sixth People's Hospital
2015-2025
Shanghai Jiao Tong University
2016-2025
Nanjing Medical University
2023-2024
Jiangsu Shengze Hospital
2023-2024
Anyang Academy of Agricultural Sciences
2023-2024
China National Rice Research Institute
2015-2022
University of Pittsburgh
2020-2022
UPMC Hillman Cancer Center
2020-2022
State Key Laboratory of Microbial Metabolism
2021
China University of Petroleum, East China
2019
Summary Grain size is an important agronomic trait in determining grain yield. However, the molecular mechanisms that determine final are not well understood. Here, we report functional analysis of a rice ( Oryza sativa L.) mutant, dwarf and small grain1 dsg1 ), which displays pleiotropic phenotypes, including grains, dwarfism erect leaves. Cytological observations revealed were mainly caused by inhibition cell proliferation. Map‐based cloning DSG 1 encoded mitogen‐activated protein kinase...
Abstract Colorectal cancer (CRC) usually creates an immunosuppressive microenvironment, thereby hindering immunotherapy response. Effective treatment options remain elusive. Using scRNA‐seq analysis in a tumor‐bearing murine model, it is found that lobeline, alkaloid from the herbal medicine lobelia, promotes polarization of tumor‐associated macrophages (TAMs) toward M1‐like TAMs while inhibiting their M2‐like TAMs. Additionally, lobeline upregulates mRNA expression secreted...
<h3>Objective</h3> miR-21 was found to be overexpressed in the colon tissues and serum of patients with UC colorectal cancer (CRC); however, exact roles colitis-associated CRC remain unclear. The aim our study investigate biological mechanisms (CAC). <h3>Design</h3> expression examined tumours 62 from China 37 neoplastic Japan Austria. functions were studied using a series vitro, vivo clinical approaches. <h3>Results</h3> levels markedly upregulated CRC, 22 CAC, mouse model CAC. Following...
Background MicroRNA-21 (miR-21) is overexpressed in most inflammatory diseases, but its physiological role gut inflammation and tissue injury poorly understood. The goal of this work to understand the miR-21 colitis damage progression intestine a genetically modified murine model. Methods Experimental was induced KO wild-type (WT) mice by 3.5% dextran sulphate sodium (DSS) administration for 7 days. Disease activity index(DAI), blood parameters, intestinal permeability, histopathologic...
Although recent studies indicate that DNA methylation contributes to the down-regulation of microRNAs (miRNAs) in colorectal cancer (CRC), this field remains largely unexplored. To identify methylation-silenced miRNAs and clarify their role CRC, we performed a microarray analysis screened for were induced CRC cells by 5-aza-2'-deoxycytidine treatment or knockdown methyltransferases. The status candidate miRNA was analysed bisulphite sequencing PCR methylation-specific PCR. We found miRNA-149...
// Yongzhi Yang 1,* , Xia 2,* Hongqi Chen 2 Leiming Hong 1 Junlan Feng Jun Zhe Chenzhang Shi Wen Wu Renyuan Gao Qing Wei 3 Huanlong Qin and Yanlei Ma Department of GI Surgery, Shanghai Tenth People's Hospital Affiliated to Tongji University, Shanghai, China Jiao Tong University Sixth Hospital, Pathology, * These authors have contributed equally this work Correspondence to: Qin, email: Ma, Wei, Keywords : colorectal cancer, perioperative probiotics treatment, post-operative outcome,...
Background Increasing infiltration of CD8 + T cells within tumor tissue predicts a better prognosis and is essential for response to checkpoint blocking therapy. Furthermore, current clinical protocols use unfractioned cell populations as the starting point transduction chimeric antigen receptors (CARs)-modified cells, but optimal subtype CAR-modified remains unclear. Thus, accurately identifying group cytotoxic lymphocytes with high antitumor efficacy imperative. Inspired by theory yin...
Colorectal cancer (CRC) is one of the most common malignant tumors, with second-highest mortality all 36 cancers worldwide. The roles fatty acid metabolism in CRC were investigated to explore potential therapeutic strategies. data files downloaded from Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. Univariate least absolute shrinkage selection operator (LASSO) Cox regression analyses used construct a prognostic risk score model metabolism-related genes for predicting...
Background The loss of tumor antigens and depletion CD8 T cells caused by the PD-1/PD-L1 pathway are important factors for immune escape. In recent years, there has been increasing research on traditional Chinese medicine in treatment. Cycloastragenol (CAG), an effective active molecule Astragalus membranaceus , found to have antiviral, anti-aging, anti-inflammatory, other functions. However, its antitumor effect mechanism not clear. Methods CAG was investigated MC38 CT26 mouse transplanted...
Colorectal tumors often create an immunosuppressive microenvironment that prevents them from responding to immunotherapy. Cannabidiol (CBD) is a non-psychoactive natural active ingredient the cannabis plant has various pharmacological effects, including neuroprotective, antiemetic, anti-inflammatory, and antineoplastic activities. This study aimed elucidate specific anticancer mechanism of CBD by single-cell RNA sequencing (scRNA-seq) ATAC (scATAC-seq) technologies. Here, we report inhibits...
Molecular subtyping is expected to enable precise treatment. However, reliable strategies for clinical application remains defective and controversial. Given the significance of tumor immune dysfunction exclusion (TIDE), we aimed develop a novel TIDE-based strategy guide personalized immunotherapy in bladder cancer (BC).
Colorectal cancer (CRC) is the third most common worldwide and has poor prognosis. To identify oncofetal proteins involved in CRC carcinogenesis, differentially expressed among fetal colorectal tissues, CRC, paired tumor-adjacent normal tissues were investigated by a two-dimensional gel electrophoresis MALDI-TOF/TOF-based proteomics approach. 42 protein spots these 22 identified MS analysis. Desmin zinc finger 829 found to be elevated tissue compared with tissue. The expression of desmin...
Abstract Colorectal cancer (CRC) is one of the most common neoplasms worldwide. However, mechanisms underlying its development are still poorly understood. Thyroid hormone Receptor Interactor 13 (TRIP13) a key mitosis regulator, and recent evidence has shown that it an oncogene. Here, we report TRIP13, which overexpressed in CRC, correlated with CEA (carcino-embryonic antigen), CA19-9 (carbohydrate antigen 19-9) pTNM (pathologic primary tumor, lymph nodes, distant metastasis) classification....
Abstract MicroRNA‐145 (miR‐145), as a tumor‐suppressive miRNA, has been demonstrated down‐regulated in colorectal cancer (CRC) cells, and could inhibit CRC cells growth. However, the molecular pathway which miR‐145 modulates malignant transformation not fully revealed. Here, we reported an intense correlation between expressions of PAK4 human cell lines. Transwell assay verified overexpression miR‐145, well knockdown PAK4, significantly suppressed migration invasion ability. The impaired...
Immune checkpoint blockade is arguably the most effective current cancer therapy approach; however, its efficacy limited to patients with “hot” tumors, warranting an approach transform “cold” tumors. Oncolytic viruses (especially properly armed ones) have positive effects on almost every aspect of cancer–immunity cycle and can change cancer–immune set point a tumor. Here, we tested whether oncolytic vaccinia virus delivering tethered interleukin 12 (IL-12) could turn tumor into while...
Colorectal cancer (CRC), a malignant tumor worldwide consists of microsatellite instability (MSI) and stable (MSS) phenotypes. Although SHP2 is hopeful target for therapy, its relationship with innate immunosuppression remains elusive. To address that, single-cell RNA sequencing was performed to explore the role in all cell types microenvironment (TME) from murine MC38 xenografts. Intratumoral cells were found be functionally heterogeneous responded significantly SHP099, allosteric...
Post-traumatic peritendinous adhesion presents a significant challenge in clinical medicine. This study proposes the use of diamond-like carbon (DLC) deposited on polylactic acid (PLA) membranes as biophysical mechanism for anti-adhesion barrier to encase ruptured tendons tendon-injured rats. The results indicate that PLA/DLC composite membrane exhibits more efficient effect than PLA membrane, with histological score decreasing from 3.12 ± 0.27 2.20 0.22 and effectiveness increasing 21.61%...
Background: Newly emerging cancer immunotherapy has led to significant progress in treatment; however, its efficacy is limited solid tumors since the majority of them are "cold" tumors. Oncolytic viruses, especially when properly armed, can directly target tumor cells and indirectly modulate microenvironment (TME), resulting "hot" These viruses be applied as a approach either alone or combination with other immunotherapies. Cytokines good candidates arm oncolytic viruses. IL-23, an IL-12...
T cells, including both CD4+ and CD8+ play a pivotal role in mediating various inflammation immune disorders. A long-standing challenge cell-based immunotherapy is to precisely inactivate or delete the pathogenic cells autoimmune diseases, selectively expand immunocompetent cell tumor other compromised situations, without inducing global immunosuppression zealous activation respectively. To achieve this, specific marker needed differentiate among rests. Indeed, recent progress of immunology...
The increase in proliferation and the lack of differentiation cancer cells resemble what occur embryonic stem during physiological process embryogenesis. There are also striking similarities behaviour between invasive placental cells. In present study, microarrays were used to analyse global expression microRNAs a human cell line (i.e. HUES-17) four colorectal (CRC) lines LoVo, SW480, HT29 Caco-2) with different metastatic potentialities. Only miR-26b was significant decreased HUES-17s LoVo...
Colorectal cancer (CRC) is the third most common worldwide and has poor prognosis. To identify proteins involved in colorectal carcinogenesis, we employed 2-DE MALDI-TOF/TOF-based proteomics approach to study differentially expressed tumor adjacent nontumor tissue samples. Samples from 10 patients were analyzed. Of 7 significantly consistently altered identified, hnRNP A1 was one of its overexpression confirmed using RT-PCR Western blot analyses. Immunohistochemical examination showed that...