A5007499183- Gut microbiota and health
- Diet and metabolism studies
- Monoclonal and Polyclonal Antibodies Research
- Nutritional Studies and Diet
- Food Security and Health in Diverse Populations
- Pediatric Hepatobiliary Diseases and Treatments
- Liver Diseases and Immunity
- Liver Disease Diagnosis and Treatment
- Mycobacterium research and diagnosis
- Pancreatic and Hepatic Oncology Research
- Systemic Lupus Erythematosus Research
- Metabolomics and Mass Spectrometry Studies
- Vasculitis and related conditions
- Atherosclerosis and Cardiovascular Diseases
- Renal Diseases and Glomerulopathies
- Galectins and Cancer Biology
- Immunodeficiency and Autoimmune Disorders
- Epigenetics and DNA Methylation
- Rheumatoid Arthritis Research and Therapies
- Cell Adhesion Molecules Research
- Aortic Disease and Treatment Approaches
- Cardiac Valve Diseases and Treatments
- Cardiomyopathy and Myosin Studies
University of Minnesota
2020-2025
Mayo Clinic
2023
Mayo Clinic in Florida
2020
Twin Cities Orthopedics
2020
Abstract Providing insight into one’s health status from a gut microbiome sample is an important clinical goal in current human research. Herein, we introduce the Gut Microbiome Health Index (GMHI), biologically-interpretable mathematical formula for predicting likelihood of disease independent diagnosis. GMHI formulated upon 50 microbial species associated with healthy ecosystems. These are identified through multi-study, integrative analysis on 4347 stool metagenomes 34 published studies...
Abstract Background Rapid advances in the past decade have shown that dysbiosis of gut microbiome is a key hallmark rheumatoid arthritis (RA). Yet, relationship between and clinical improvement RA disease activity remains unclear. In this study, we explored patients with to identify features are associated with, as well predictive of, minimum clinically important (MCII) activity. Methods We conducted retrospective, observational cohort study on diagnosed 1988 2014. Whole metagenome shotgun...
Recent advancements in translational gut microbiome research have revealed its crucial role shaping predictive healthcare applications. Herein, we introduce the Gut Microbiome Wellness Index 2 (GMWI2), an enhanced version of our original GMWI prototype, designed as a standardized disease-agnostic health status indicator based on taxonomic profiles. Our analysis involves pooling existing 8069 stool shotgun metagenomes from 54 published studies across global demographic landscape (spanning 26...
BACKGROUND: Pharmacological treatments for fibrocalcific aortic valve stenosis (FCAVS) have been elusive >50 years. Here, we tested the hypothesis that reactivation of oxidized sGC (soluble guanylate cyclase), primary receptor nitric oxide, with ataciguat is a safe and efficacious strategy to slow progression FCAVS. METHODS: We used quantitative real-time reverse transcription polymerase chain reaction, Western blotting, immunohistochemistry characterize signaling biological effects on...
Patients with rheumatoid arthritis (RA) can test either positive or negative for circulating anti-citrullinated protein antibodies (ACPA) and are thereby categorized as ACPA-positive (ACPA+) ACPA-negative (ACPA-), respectively. In this study, we aimed to elucidate a broader range of serological autoantibodies that could further explain immunological differences between patients ACPA+ RA ACPA- RA. On serum collected from adult (n = 32), 30), matched healthy controls used highly multiplex...
The relationship between primary biliary cholangitis (PBC), a chronic cholestatic autoimmune liver disease, and the peripheral immune system remains to be fully understood. Herein, we performed first mass cytometry (CyTOF)-based, immunophenotyping analysis of in PBC at single-cell resolution. CyTOF was on blood mononuclear cells (PBMCs) from patients (n = 33) age-/sex-matched healthy controls obtain cell abundance marker expression profiles. Hierarchical clustering methods were applied...
Abstract Summary We recently introduced the Gut Microbiome Wellness Index (GMWI), a stool metagenome-based indicator for assessing health by determining likelihood of disease given state one’s gut microbiome. The calculation our wellness index depends on relative abundances health-prevalent and health-scarce species. Encouragingly, GMWI has already been utilized in various studies focusing differences microbiome between cases controls. Herein, we introduce GMWI-webtool, user-friendly browser...
This study aimed to identify plasma proteomic signatures that differentiate active and inactive giant cell arteritis (GCA) from non-disease controls. By comprehensively profiling the proteome of both patients with GCA controls, we proteins (1) distinguish controls (2) associate disease activity in GCA.
Abstract The relationship between Primary Biliary Cholangitis (PBC), a chronic cholestatic autoimmune liver disease, and the peripheral immune system remains to be fully understood. Herein, we performed first mass cytometry (CyTOF)-based, immunophenotyping analysis of in PBC at single-cell resolution. CyTOF was on blood mononuclear cells (PBMCs) from patients (n=33) age-/sex-matched healthy controls obtain cell abundance marker expression profiles. Hiearchical clustering methods were applied...
Abstract Background Rapid advances in the past decade have shown that dysbiosis of gut microbiome is a key hallmark rheumatoid arthritis (RA). Yet, relationship between and clinical improvement RA disease activity remains unclear. In this study, we explored patients with to identify features are associated with, as well predictive of, minimum clinically important (MCII) activity. Methods Whole metagenome shotgun sequencing was performed on 64 stool samples, which were collected from 32 at...
Abstract Recent advancements in human gut microbiome research have revealed its crucial role shaping innovative predictive healthcare applications. We introduce Gut Microbiome Wellness Index 2 (GMWI2), an advanced iteration of our original GMWI prototype, designed as a robust, disease-agnostic health status indicator based on taxonomic profiles. Our analysis involved pooling existing 8069 stool shotgun metagenome data across global demographic landscape to effectively capture biological...
ABSTRACT The development of a biologically-interpretable and robust metric that provides clear insight into the general health status (i.e. healthy or non-healthy) one’s gut microbiome remains an important target in human research. We introduce Gut Microbiome Health Index (GMHI), mathematical formula determines degree to which profile reflects good adverse health. GMHI was formulated based on microbial species specific ecosystems. These were identified through multi-study, integrative...
Abstract Summary We recently introduced the Gut Microbiome Health Index (GMHI), a stool-based indicator for monitoring health given state of one’s gut microbiome. GMHI depends on health-prevalent and health-scarce species determined validated using pooled dataset 5,026 stool shotgun metagenomic samples from 43 independent studies. Encouragingly, has already been utilized in various studies focusing identifying differences microbiome between cases controls. However, current computational...
Abstract Objectives Patients with rheumatoid arthritis (RA) can test either positive or negative for anti-citrullinated protein antibodies (ACPA), and are thereby ACPA-positive (ACPA+) ACPA-negative (ACPA–), respectively. Through comprehensive profiling of autoantibodies in serum, we aimed to identify that differentially abundant between patients ACPA+ RA ACPA– RA, also those significantly associated clinical disease activity. Methods Serum was collected from ( n = 32), 30), healthy controls...