A5016534786- Lipid Membrane Structure and Behavior
- Cellular transport and secretion
- Bacterial Genetics and Biotechnology
- Biofuel production and bioconversion
- Botulinum Toxin and Related Neurological Disorders
- Bacteriophages and microbial interactions
- Microbial Metabolic Engineering and Bioproduction
- Fungal and yeast genetics research
- Calcium signaling and nucleotide metabolism
- Biotin and Related Studies
- Nanopore and Nanochannel Transport Studies
- Endoplasmic Reticulum Stress and Disease
- Neurological disorders and treatments
- Mitochondrial Function and Pathology
- Antibiotic Resistance in Bacteria
- Biochemical Acid Research Studies
- Advanced Fluorescence Microscopy Techniques
- Caveolin-1 and cellular processes
- Evolution and Genetic Dynamics
- Signaling Pathways in Disease
- Electrocatalysts for Energy Conversion
- Advancements in Solid Oxide Fuel Cells
- Analytical Chemistry and Chromatography
- Erythrocyte Function and Pathophysiology
- Vibrio bacteria research studies
Université Paris Cité
2019-2023
Inserm
2021-2023
École Normale Supérieure - PSL
2019-2023
Sorbonne Université
2019-2023
Centre National de la Recherche Scientifique
2019-2023
Institut de Psychiatrie et Neurosciences de Paris
2021-2023
École Normale Supérieure
2023
Université Paris Sciences et Lettres
2019-2022
Sungkyunkwan University
2010-2019
Sorbonne Paris Cité
2019
We show that 3-[4-(4-methoxyphenyl)piperazin-1-yl]piperidin-4-yl biphenyl-4-carboxylate (C10), screened out of a chemical library, selectively kills bacterial persisters tolerate antibiotic treatment but does not affect normal antibiotic-sensitive cells. C10 led to antibiotic-induced cell death by causing reversion This work is the first demonstration in which eradication based on single-chemical supplementation. The should be versatile elucidating mechanism persistence.
ABSTRACT Bacterial persisters are a small fraction of quiescent cells that survive in the presence lethal concentrations antibiotics. They can regrow to give rise new population has same vulnerability antibiotics as did parental population. Although formation bacterial various been documented, molecular mechanisms by which these tolerate still controversial. We found amplification fumarate reductase operon ( FRD ) Escherichia coli led higher frequency persister formation. The E. was...
Abstract Membrane-disrupting agents that selectively target virus versus host membranes could potentially inhibit a broad-spectrum of enveloped viruses, but currently such antivirals are lacking. Here, we develop nanodisc incorporated with decoy receptor inhibits infection. Mechanistically, nanodiscs carrying the viral sialic acid bind to influenza virions and co-endocytosed into cells. At low pH in endosome, rupture envelope, trapping RNAs inside endolysosome for enzymatic decomposition. In...
Experimental setups to produce and monitor model membranes have been successfully used for decades brought invaluable insights into many areas of biology. However, they all limitations that prevent the full in vitro mimicking monitoring most biological processes. Here, a suspended physiological bilayer-forming chip is designed from 3D-printing techniques. This can be simultaneously integrated confocal microscope path-clamp amplifier. It composed poly(dimethylsiloxane) consists ≈100 µm hole,...
Apolipoproteins L1 and L3 (APOLs) are associated at the Golgi with membrane fission factors phosphatidylinositol 4-kinase-IIIB (PI4KB) non-muscular myosin 2A. Either APOL1 C-terminal truncation (APOL1Δ) or APOL3 deletion (APOL3-KO [knockout]) reduces PI4KB activity triggers actomyosin reorganization. We report that APOL3, but not APOL1, controls through interaction neuronal calcium sensor-1 calneuron-1. Both APOLs present in Golgi-derived autophagy-related protein 9A vesicles, which involved...
Anti-allergic effects of dietary polyphenols were extensively studied in numerous allergic disease models, but the molecular mechanisms anti-allergic by remain poorly understood. In present study, we show that release granular cargo molecules, contained distinct subsets granules mast cells, is specifically mediated two sets SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) proteins, and various differentially inhibit formation those complexes. Expression analysis...
Significance Using our recently designed microfluidic setup, we investigated the early stage of SNAREpin-induced fusion. We discovered existence subsecond transient fusion pores with a well-defined subnanometer size that occur when one or two SNAREpins are mediating vesicle In contrast, is mediated by three SNAREpins, pore reaches diameter larger than 1.5 nm and expands spontaneously indefinitely. These results quantitatively explain need for complex machinery to ensure submillisecond...
Membrane fusion is mediated by the SNARE complex which formed through a zippering process. Here, we developed chemical controller for progress of membrane fusion. A hemifusion state was arrested polyphenol myricetin binds to complex. The arrest rescued an enzyme laccase that removes from metastable and long-lived with decay constant 39 min. This applied delineate how Ca(2+) stimulates fusion-pore formation in millisecond time scale. We found, using single-vesicle assay, such myricetin-primed...
Neurotransmitters are released within a millisecond after Ca2+ arrives at an active zone. However, the vesicle fusion pathway underlying this synchronous release is yet to be understood. At center of controversy whether hemifusion, in which outer leaflets merged while inner still separated, on-pathway or off-pathway product Ca2+-triggered exocytosis. Using single assay, we recently demonstrated that hemifusion intermediate immediately proceeds full upon triggering. It has been shown...
Synucleinopathies are neurological diseases that characterized by the accumulation of aggregates a cytosolic protein, α-synuclein, at plasma membrane. Even though pathological role protein is established, mechanism which it damages neurons remains unclear due to difficulty correctly mimic membrane in vitro. Using microfluidic setup composition membrane, including asymmetry two leaflets, recapitulated, we demonstrate triple action α-synuclein on First, changes topology inducing pores discrete...
Botulinum neurotoxins (BoNTs) are popularly used to treat various diseases and for cosmetic purposes. They act by blocking neurotransmission through specific cleavage of soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins. Recently, several polyphenols were shown interfere with SNARE complex formation wedging into the hydrophobic core interface, thereby leading reduced neuroexocytosis.In order find industrially-viable plant extract that functions like BoNT,...
Abstract This paper presents a promising approach to reduce the quantity of Pt required in cathodes for high‐temperature proton exchange membrane (PEM) fuel cells. Based on preliminary experiments, thermally stable Si 0.97 Al 0.03 C and Sn 0.95 In 0.05 P 2 O 7 were selected as promoter ionomer, respectively. particles (∼40 nm) (∼45 successfully produced carbon support. (∼9 selectively impregnated vicinity ionomer. Polarisation measurements revealed that Pt‐Sn ‐Si C/C cathode exhibited much...
Tail-anchored (TA) proteins contain a single C-terminal transmembrane domain (TMD) that is captured by the cytosolic Get3 in yeast (TRC40 humans). delivers TA to Get1/2 complex for insertion into endoplasmic reticulum (ER) membrane. How mediates of TMDs membrane poorly understood. Using bulk fluorescence and microfluidics assays, we show forms an aqueous channel reconstituted bilayers. We estimate diameter be ∼2.5 nm wide, corresponding circumference two complexes. find binding can seal...