A5060750921- Liver Disease Diagnosis and Treatment
- Hemoglobin structure and function
- Mitochondrial Function and Pathology
- Pancreatitis Pathology and Treatment
- Inflammation biomarkers and pathways
- Neonatal Health and Biochemistry
- Metabolism and Genetic Disorders
- Receptor Mechanisms and Signaling
- Hemoglobinopathies and Related Disorders
- Neuropeptides and Animal Physiology
- Immune cells in cancer
- Pancreatic function and diabetes
- Endoplasmic Reticulum Stress and Disease
- Apelin-related biomedical research
- Erythrocyte Function and Pathophysiology
- Liver Diseases and Immunity
- Diabetes and associated disorders
University of Southern Denmark
2020-2023
Background and Aims: Reliable noninvasive biomarkers are an unmet clinical need for the diagnosis of NASH. This study investigates diagnostic accuracy circulating triggering receptor expressed on myeloid cells 2 (plasma TREM2) as a biomarker NASH in patients with NAFLD elevated liver stiffness. Approach Results: We collected cross‐sectional, data including biopsies from derivation ( n = 48) validation cohort 170) stiffness measurement (LSM ≥ 8.0 kPa). Patients activity scores (NAS) ≥4 were...
Apolipoproteins L1 and L3 (APOLs) are associated at the Golgi with membrane fission factors phosphatidylinositol 4-kinase-IIIB (PI4KB) non-muscular myosin 2A. Either APOL1 C-terminal truncation (APOL1Δ) or APOL3 deletion (APOL3-KO [knockout]) reduces PI4KB activity triggers actomyosin reorganization. We report that APOL3, but not APOL1, controls through interaction neuronal calcium sensor-1 calneuron-1. Both APOLs present in Golgi-derived autophagy-related protein 9A vesicles, which involved...
Macrophages play an important role in the development of nonalcoholic fatty liver disease (NAFLD) and its progression to steatohepatitis (NASH). In this study, we investigated hepatic expression macrophage scavenger receptor CD163 plasma level shed soluble form (sCD163) patients with obesity NASH, non-NASH NAFLD (NAFL), or healthy livers (no NAFLD).
Haptoglobin (Hp) is an abundant plasma protein scavenging hemoglobin (Hb) via CD163 on macrophages. This process consumes Hp, which therefore negatively correlates to hemolysis. However, exact measurements of Hp levels are complicated by different phenotypes (Hp1-1, Hp2-1, and Hp2-2) forming oligomeric states with differences in immunoreactivity. In addition, humans have immune-cross-reactive Hp-related protein. the present study, we developed Hp-specific monoclonal antibodies for accurate...
Haptoglobin-related protein (Hpr) is a plasma with high sequence similarity to haptoglobin (Hp). Like Hp, Hpr also binds hemoglobin (Hb) affinity, but it does not bind the Hb-Hp receptor CD163 on macrophages. The concentration markedly lower than Hp in and its regulation understood. In present study, we have developed non-crossreactive antibodies analyze 112 samples from anonymized individuals compared Hp. results show that correlated concentrations (rho = 0.46, p .0001). accounts for...
Apolipoproteins-L1 and -L3 (APOLs) are associated at the Golgi membrane with phosphatidylinositol-4-kinase-IIIB (PI4KB) non-muscular-myosin-2A (NM2A). Either APOL1 C-terminal truncation (APOL1Δ) or APOL3 deletion (APOL3-KO) reduces PI4KB activity triggers actomyosin reorganization. We report that APOL3, but not APOL1, controls through interaction neuronal-calcium-sensor-1 (NCS1) calneuron-1 (CALN1). Both APOLs present in Golgi-derived autophagy-related-protein-9A (ATG9A) vesicles, involved...