A5032395535- Cardiac electrophysiology and arrhythmias
- Ion channel regulation and function
- Cardiac Arrhythmias and Treatments
- Cardiac pacing and defibrillation studies
- Receptor Mechanisms and Signaling
- Cardiovascular Effects of Exercise
- Cardiomyopathy and Myosin Studies
- Atrial Fibrillation Management and Outcomes
- ECG Monitoring and Analysis
- Heart Rate Variability and Autonomic Control
- Neuroscience and Neuropharmacology Research
- Analytical Methods in Pharmaceuticals
- Neuroscience and Neural Engineering
- Cardiac Structural Anomalies and Repair
- Cardiac Fibrosis and Remodeling
- Signaling Pathways in Disease
- Cardiovascular Function and Risk Factors
- Cardiac Imaging and Diagnostics
- Plant-based Medicinal Research
- Inorganic and Organometallic Chemistry
- Sports injuries and prevention
- Cardiac Ischemia and Reperfusion
- Analytical Chemistry and Sensors
- Electrochemical Analysis and Applications
- Antibiotics Pharmacokinetics and Efficacy
University of Copenhagen
2024
University of Calgary
2014-2023
Libin Cardiovascular Institute of Alberta
2013-2022
Universitätsklinikum Würzburg
2019
Institute of Solution Chemistry
2019
San Diego Cardiac Center
2018
Hospital General Universitario Gregorio Marañón
2017
Centro de Investigación en Red en Enfermedades Cardiovasculares
2017
Calgary Laboratory Services
2011-2016
Derriford Hospital
2015
BACKGROUND Patients with a history of class Ia drug-induced torsade de pointes have been treated chronic amiodarone without recurrence despite comparable prolongation the QT interval. We hypothesized that in such patients, drugs cause nonhomogeneous cardiac repolarization times, whereas causes homogeneous times. METHODS AND RESULTS Thirty-eight consecutive patients who received both drug therapy and were evaluated. Standard 12-lead ECGs at baseline during each used to calculate precordial...
Abstract Tubulointerstitial inflammation and fibrosis are strongly associated with the outcome of chronic kidney disease. We recently demonstrated that NOD-like receptor, pyrin domain containing-3 (NLRP3) contributes to renal inflammation, injury, following unilateral ureteric obstruction in mice. NLRP3 expression tubular epithelial cells (TECs) was found be an important component experimental disease pathogenesis, although biology is unknown. In human mouse primary TECs, increased response...
This study reports that the affinity of HERG1 A for dofetilide is decreased from 0.125 +/- 0.003 microM wild-type (WT) channels to 15 3 F656V, a mutation in COOH-terminal half S6. Similarly, IC(50) quinidine was increased 8 4 WT 219 65 F656V mutation, whereas external tetraethylammonium similar (51 10 mM) and (36 mM, NS). Kinetics onset inactivation but kinetics deactivation, activation, recovery differed WT. However, mutations nearby amino acids S6 more strikingly altered had little effect...
Expression of cardiac transient outward current and inwardly rectifying K + is age dependent. However, little known about age-related changes in delayed rectifier (I , with rapidly slowly activating components, I Kr Ks respectively). Accordingly, the purpose present study was to assess developmental channels fetal, neonatal, adult mouse ventricles. Three techniques were used: conventional microelectrode measure action potential, voltage clamp record macroscopic currents radioligand assay...
Abstract Mutants of HERG, the human form ERG (the ether-a-go-go –related K + channel gene), are responsible for some forms long-QT syndrome, an abnormality cardiac repolarization. HERG was cloned from brain and has properties similar but not identical to rapidly activating component native current ( I Kr ). We identified in mouse alternatively processed (MERG B) that is expressed abundantly heart only trace amounts brain. MERG B a unique 36–amino acid NH 2 -terminal domain strongly basic...
There is controversy over whether therapy to prevent ventricular tachyarrhythmias should be selected noninvasively (by trying drugs and monitoring the patient electrocardiographically) or invasively selecting a drug that prevents induction of arrhythmia by programmed stimulation). We randomly assigned 57 patients with symptomatic demonstrable either invasively. The involved were sustained tachycardia (35 patients), nonsustained hypotension (15 fibrillation (7 patients). noninvasive approach...
A RIA for alpha-human atrial natriuretic peptide (alpha hANP) in plasma was developed and used to study the immunoreactive components secreted by heart circulating peripheral venous plasma. The assay [125I]diiodotyrosyl-alpha hANP, purified high pressure liquid chromatography (HPLC), a C-terminal-specific antiserum purchased from Peninsula Laboratories. Serial dilution curves of coronary sinus samples were parallel with standard curve, but significant nonparallelism found low...
Caffeine has long been used as a pharmacological probe for studying RyR (ryanodine receptor)-mediated Ca(2+) release and cardiac arrhythmias. However, the precise mechanism by which caffeine activates RyRs is elusive. In present study, we investigated effects of on spontaneous response single RyR2 (cardiac RyR) channels to luminal or cytosolic Ca(2+). We found that HEK-293 cells (human embryonic kidney cells) expressing displayed partial 'quantal' in repetitive additions submaximal...
Heart failure is associated with a low-grade and chronic cardiac inflammation that impairs function; however, the mechanisms by which this sterile occurs in structural heart disease remain poorly defined. Cardiac-specific heterozygous overexpression of calcineurin transgene (CNTg) mice results hypertrophy, inflammation, apoptosis ventricular dilatation. We hypothesized activation Nlrp3 inflammasome, an intracellular danger-sensing pathway required for processing pro-inflammatory cytokine...
Nonmicrobial inflammation contributes to CKD progression and fibrosis. Absent in melanoma 2 (AIM2) is an inflammasome-forming receptor for double-stranded DNA. AIM2 expressed the kidney activated mainly by macrophages. We investigated potential pathogenic role of inflammasome disease. In kidneys from patients with diabetic or nondiabetic CKD, immunofluorescence showed expression glomeruli, tubules, infiltrating leukocytes. a mouse model unilateral ureteral obstruction (UUO), Aim2 deficiency...
Induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) capture patient-specific genotype-phenotype relationships, as well cell-to-cell variability of cardiac electrical activity Computational modelling and simulation provide a high throughput approach to reconcile multiple datasets describing physiological variability, also identify vulnerable parameter regimes We have developed whole-cell model iPSC-CMs, composed single exponential voltage-dependent gating variable rate constants,...
The term idiopathic ventricular fibrillation (IVF) describes survivors of unexplained cardiac arrest (UCA) without a specific diagnosis after clinical and genetic testing. Previous reports have described subset IVF individuals with arrhythmia initiated by short-coupled trigger premature contractions (PVCs) for which the (SCVF) has been proposed. aim this article is to establish phenotype frequency SCVF in large cohort UCA survivors.We performed multicentre study including consecutive from...
The expression of calreticulin, a Ca(2+)-binding chaperone the endoplasmic reticulum, is elevated in embryonic heart, and because impaired cardiac development, knockout Calreticulin gene lethal during embryogenesis. downregulated after birth. Here we have investigated physiological consequences continued high calreticulin postnatal by producing transgenic mice that overexpress protein heart. These animals exhibit decreased systolic function inward I(Ca,L), low levels connexin43 connexin40,...
Acute mountain sickness (AMS) may affect individuals who (rapidly) ascend to altitudes higher than 2,000-3,000 m. A more serious consequence of rapid ascent be high-altitude pulmonary edema, a hydrostatic edema associated with increased capillary pressures. Acetazolamide is effective against AMS, possibly by increasing ventilation and cerebral blood flow (CBF). In animals, it inhibits hypoxic vasoconstriction.We examined the influence acetazolamide on response hypoxia ventilation, CBF,...