Background —Newly discovered circulating peptides, N-terminal pro–brain natriuretic peptide (N-BNP) and adrenomedullin (ADM), were examined for prediction of cardiac function prognosis compared with previously reported markers in 121 patients myocardial infarction. Methods Results —The association between radionuclide left ventricular ejection fraction (LVEF) N-BNP at 2 to 4 days ( r =−.63, P <.0001) 3 5 months =−.58, after infarction was comparable that C-terminal BNP far stronger than...

OBJECTIVE Human brain natriuretic peptide‐32 (BNP) (i.e. proBNP(77–108)), the mature form of BNP and secreted predominantly by cardiac ventricle, is formed from a high molecular weight precursor, proBNP(1–108). We have recently identified aminoterminal proBNP(1–76) (NT‐proBNP) in human plasma but its source, metabolism production circulatory disorders are unknown. investigated relationship between immunoreactive (IR) NT‐proBNP BNP‐32 normal hypertensive subjects patients with impairment, as...

Background— A recent landmark report has demonstrated that plasma B-type natriuretic peptide (BNP) measured in acute coronary syndromes independently predicts mortality, heart failure, and new myocardial infarction. After cardiac injury, left ventricular ejection fraction (LVEF) is also of prognostic significance plays a major role determining the therapeutic response. Methods Results— The present first from substantial (n=666) cohort patients with infarction to test utility concurrent...

Plasma neurohormones were analyzed for prediction of adverse outcomes and response to treatment in 415 patients with ischemic left ventricular dysfunction randomly assigned receive carvedilol or placebo.Atrial natriuretic peptide, brain peptide (BNP), norepinephrine (NE) levels above the group median associated increased mortality rates heart failure. On multivariate analysis, both BNP NE interacted predict death failure independent age, New York Heart Association class, ejection fraction....

Abstract —Cardiac natriuretic peptides, especially amino terminal pro–Brain Natriuretic Peptide (NT-proBNP), are emerging as powerful circulating markers of cardiac function. However, the in vivo secretion and elimination (t1/2) these peptides during acute volume overload have not been studied. We present first report based on deconvolution analysis endogenous ovine plasma levels measured by specific radioimmunoassay. Four normal, conscious sheep underwent rapid right ventricular pacing (225...

A RIA for human brain natriuretic peptide (BNP) was developed. Both BNP and atrial (ANP) were extracted from plasma with Vycor glass powder (71% recovery BNP). The assay had a minimum detection limit of 0.45 fmol/tube an IC50 9 fmol/tube. within-assay coefficients variation 11.4% at 4 pmol/L 3.2% 22 pmol/L, the between-assay coefficient 11% 24 pmol/L. There no significant loss immunoreactive (IR)-BNP in samples stored -80 C weeks. Low rates labeled IR-BNP degradation occurred EDTA incubated...

A RIA for alpha-human atrial natriuretic peptide (alpha hANP) in plasma was developed and used to study the immunoreactive components secreted by heart circulating peripheral venous plasma. The assay [125I]diiodotyrosyl-alpha hANP, purified high pressure liquid chromatography (HPLC), a C-terminal-specific antiserum purchased from Peninsula Laboratories. Serial dilution curves of coronary sinus samples were parallel with standard curve, but significant nonparallelism found low...

OBJECTIVE--To document regional plasma concentrations of brain natriuretic factor (BNF) and their relations to concurrent atrial factor, cyclic guanosine monophosphate, haemodynamic state. DESIGN--Regional blood sampling from a systemic artery vein, renal coronary sinus together with indices in patients coming forward for left right cardiac catheterisation. SETTING--Tertiary referral centre. PATIENTS--22 consecutive unselected catheterisation or electrophysiological studies the course...

1. Since important interrelationships between haemodynamic and hormone indices are possible in cardiac failure, measurements of output, mean pulmonary artery pressure, plasma renin activity, angiotensin II aldosterone were carried out before during acute chronic frusemide therapy patients with oedematous heart failure who had been given digoxin. 2. Cardiac output fell significantly 90 min after injection, then returned to baseline. Mean pressure declined steadily throughout the 4 h...

C-type natriuretic peptide (CNP) is an important regulator of skeletal growth. Loss-of-function mutations affecting the CNP receptor receptor-B (gene NPR2) cause autosomal recessive dysplasia, acromesomelic Maroteaux type (AMDM). The phenotype heterozygous carriers NPR2 less clear.The objective study was to determine phenotypic features mutations.This a case-control from general community.Thirty-nine members family in which one member has AMDM were studied.This observational study.The...

Five related phenotypic females, representing three different sibships in the same family, were found to have pure gonadal dysgenesis, which was confirmed by finding of streak gonads at laparotomy each case. Cytogenetic studies a number tissues showed normal male (XY) karyotype all affected members, none whom had signs Turner's syndrome. Hormone carried out two five cases indicated negligible estrogen excretion and no response stimulus human chorionic gonadotrophin. No evidence testicular...

C-Type natriuretic peptide (CNP) is a recently identified member of the family with potent vasodepressor activity in experimental animals. Specific CNP receptors and gene transcripts have been human vascular tissues, but bioactivity metabolism humans are unknown. Accordingly, we studied renal, hormonal, hemodynamic responses nine normal men (seated, nonfasting) receiving morning infusion (2 h) synthetic CNP-22 (5 pmol/kg.min) or placebo single blind, random order. To determine action, effect...

<h3>Objective</h3> To determine the relations of plasma levels brain natriuretic peptide (BNP), atrial factor (ANF), N-terminal ANF (N-ANF), cyclic guanosine monophosphate (cGMP; cardiac second messenger), and catecholamines to left ventricular function prognosis in patients admitted with acute myocardial infarction. <h3>Design</h3> Plasma hormones (radionuclide ventriculography) were measured 1–4 days after infarction 220 a single coronary care unit. Radionuclide scanning was repeated 3–5...

To determine the renal, endocrine and haemodynamic effects of an orally active inhibitor neutral endopeptidase EC 3.4.24.11 in essential hypertension.Two groups 12 white male patients with hypertension were treated candoxatril at 25 mg every h (group 1) or 200 2) for 5 days double-blind, placebo-controlled, crossover studies.Candoxatril enhanced natriuresis over initial 48 treatment. Twenty-four-hour diurnal hormone profiles (day 4) showed modest elevations plasma atrial natriuretic factor...

The effect of 60-min constant iv infusions αhuman atrial natriuretic peptide (αhANP; 200 μg), sufficient to increasethe steady state venous plasma αhANP concentration levels found inpatients with some circulatory disorders, was studied in six normal men equilibrated on a high sodium diet (200 mmol daily) and again when low intake (10 daily). In each instance, the responsestoαhANP were compared those control given preceding day. mean immunoreactive ANP during 320 pmol/liter same both diets....

Distinguishing surgically remedial forms from other causes of primary aldosteronism (PA) may be difficult, and it is made more challenging by the earlier detection milder disease. The technical demands bilateral adrenal vein sampling (AVS)—increasingly advocated for localizing a unilateral autonomous lesion (UAL)— lack agreed criteria establishing autonomy, add further to diagnostic challenge. This retrospective review 49 hypokalemic patients with unequivocal PA (41 proven UAL, eight...

Repeated acute or chronic exposure to a particular stress results in adaptation whereby the hypothalamopituitary adrenal (HPA) axis becomes less responsive subsequent continued that stress. To investigate adaptive changes occur HPA response humans, we studied effect of exercise on basal activity six highly trained male ultramarathon athletes and healthy controls matched for body mass index. After 3-5 d abstention from intense physical activity, subjects were admitted metabolic study ward at...