A5016963599- Liver Disease Diagnosis and Treatment
- Alcohol Consumption and Health Effects
- Liver physiology and pathology
- Endoplasmic Reticulum Stress and Disease
- Drug-Induced Hepatotoxicity and Protection
- interferon and immune responses
- Eicosanoids and Hypertension Pharmacology
- Phagocytosis and Immune Regulation
- Peroxisome Proliferator-Activated Receptors
- Liver Disease and Transplantation
- NF-κB Signaling Pathways
- Gastrointestinal Tumor Research and Treatment
- Ferroptosis and cancer prognosis
- Vascular Tumors and Angiosarcomas
- Cholesterol and Lipid Metabolism
- Autophagy in Disease and Therapy
- Botulinum Toxin and Related Neurological Disorders
- Immune cells in cancer
- Gastric Cancer Management and Outcomes
- FOXO transcription factor regulation
- Vascular anomalies and interventions
- Diabetes and associated disorders
- Nerve injury and regeneration
- RNA Research and Splicing
- Cell death mechanisms and regulation
Universidad Complutense de Madrid
2021-2024
Cleveland Clinic
2016-2021
Cleveland Clinic Lerner College of Medicine
2015-2019
Universidad Autónoma de Madrid
2013-2016
Consejo Superior de Investigaciones Científicas
2012-2016
Instituto de Investigaciones Biomédicas Sols-Morreale
2013-2016
Instituto de Biomedicina y Biotecnología de Cantabria
2016
Universidad de Las Palmas de Gran Canaria
2016
Hospital General Universitario Gregorio Marañón
2005-2007
Multiple pathways of programmed cell death are important in liver homeostasis. Hepatocyte is associated with progression nonalcoholic fatty disease, and inhibition apoptosis partially protects against injury response to a high‐fat diet (HFD). However, the contribution necroptosis, caspase‐independent pathway death, HFD‐induced not known. Wild‐type C57BL/6 receptor interacting protein (RIP) 3 −/− mice were randomized chow or HFD. HFD‐fed increased expression RIP3, master regulator as well...
Since it was first described by the German anatomist and histologist, Joseph Hugo Vincenz Disse, structure functions of space a thin perisinusoidal area between endothelial cells hepatocytes filled with blood plasma, have acquired great importance in liver disease. The Disse is home for hepatic stellate (HSCs), major fibrogenic players liver. Quiescent HSCs (qHSCs) store vitamin A, upon activation they lose their retinol reservoir become activated. Activated (aHSCs) are responsible secretion...
Hepatocellular death contributes to progression of alcohol–associated (ALD-associated) and non–alcohol-associated (NAFL/NASH) liver diseases. However, receptor-interaction protein kinase 3 (RIP3), an intermediate in necroptotic cell death, injury murine models ALD but not NAFL/NASH. We show here that a differential role for mixed-lineage domain–like (MLKL), the downstream effector RIP3, versus NAFL/NASH RIP1-RIP3-MLKL can be used as biomarkers distinguish alcohol-associated hepatitis (AH)...
Cot/tpl2 is the only MAP3K that activates MKK1/2-Erk1/2 in Toll-like receptor-activated macrophages. Here we show regulates RSK, S6 ribosomal protein, and 4E-BP phosphorylation after stimulation of bone marrow-derived macrophages with lipopolysaccharide (LPS), poly I:C, or zymosan. The dissociation 4E-BP-eIF4E complex, a key event cap-dependent mRNA translation initiation, dramatically reduced LPS-stimulated Cot/tpl2-knockout (KO) versus wild-type (Wt) Accordingly, LPS activation, increased...
Map3k8 (Cot/Tpl2) activates the MKK1/2-ERK1/2, MAPK pathway downstream from interleukin-1R, tumor necrosis factor-αR, NOD-2R (nucleotide-binding oligomerization domain-like 2R), adiponectinR, and Toll-like receptors. plays a key role in innate adaptive immunity influences inflammatory processes by modulating functions of different cell types. However, its atherogenesis remains unknown. In this study, we analyzed kinase pathology.We show here that deficiency results smaller numbers...
Interferon regulatory factor 3 (IRF3) has both transcriptional and nontranscriptional functions. Transcriptional activity is dependent on serine phosphorylation of IRF3, while transcription‐independent IRF3‐mediated apoptosis requires ubiquitination. IRF3 also binds to inhibitor nuclear kappa B kinase (IKKβ) in the cytosol, restricting translocation p65. IRF3‐deficient mice are highly sensitive high‐fat diet (HFD)‐induced liver injury; however, it not known if and/or confers protection....
Whereas the main function of APN is to enhance insulin activity, it also involved in modulating macrophage phenotype. Here, we demonstrate that at physiological concentrations, activates Erk1/2 via IKKβ-p105/NF-κΒ1-Cot/tpl2 intracellular signal transduction cassette macrophages. In peritoneal macrophages stimulated with APN, Cot/tpl2 influences ability phagocytose beads. However, did not modulate known capacity decrease lipid content response treatment oxLDL or acLDL. A microarray analysis...
Toll-like receptor 4 (TLR4) is critical for ethanol (EtOH)-induced liver injury. TLR4 signaling mediated by 2 proximal adaptor molecules: myeloid differentiation primary response protein (MyD88) and TLR-domain-containing adaptor-inducing interferon-β (TRIF). Studies utilizing global knockouts of MyD88 TRIF identified a predominant role in the progression EtOH-induced In contrast, IL-1 receptor, which signals solely via pathway, also known to mediate We postulated that cell-specific cells...
We aimed to identify a microRNA (miRNA)-E3 ubiquitin ligase regulatory network for protein substrates enriched in cell death pathways and investigate the underlying molecular mechanisms alcohol-associated hepatitis (AH). An miRNA-E3 was constructed using integrated bioinformatics analysis. Differentially expressed hub miRNAs (GSE59492) their validated miRNA target genes (GSE28619) were identified liver of patients with AH compared healthy controls. Liver samples from individuals mice exposed...
Lately, many countries have restricted or even banned transfat, and palm oil has become a preferred replacement for food manufacturers. Whether is potentially an unhealthy mainly due to its high content of saturated Palmitic Acid (PA) matter debate. The aim this study was test whether qualitative aspects diet such as levels PA the fat source are risk factors Metabolic Syndrome (MS) Associated Fatty Liver Disease (MAFLD).C57BL/6 male mice were fed 14 weeks with three types Western (WD): 1....
It has been assumed that connections between the postparotid terminal branches of facial nerve are purely motor. However, nature their fibers remains unexplored. The aim this study is to determine whether these comprise motor exclusively. In total 17 were obtained from 13 different nerves. Choline acetyltransferase antibody (ChAT) was used stain in and or not all them All contained ChAT positive negative fibers. average number overall 287 (84-587) proportion 63% (37.7%-91.5%). 29% nerves,...
Expression of P21, encoded by the