A5030335749- Liver Disease Diagnosis and Treatment
- Diet, Metabolism, and Disease
- Pancreatitis Pathology and Treatment
- Liver physiology and pathology
- Liver Disease and Transplantation
- Diet and metabolism studies
- Gut microbiota and health
- Kruppel-like factors research
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Hepatocellular Carcinoma Treatment and Prognosis
- Dietary Effects on Health
- Peroxisome Proliferator-Activated Receptors
- Drug Transport and Resistance Mechanisms
- Liver Diseases and Immunity
- Alcohol Consumption and Health Effects
- Endoplasmic Reticulum Stress and Disease
- Celiac Disease Research and Management
- Organ Transplantation Techniques and Outcomes
- Pediatric Hepatobiliary Diseases and Treatments
- Cancer Mechanisms and Therapy
- Drug-Induced Hepatotoxicity and Protection
- Digestive system and related health
- Genomics, phytochemicals, and oxidative stress
- Lipid metabolism and biosynthesis
- Vitamin C and Antioxidants Research
Ruhr University Bochum
2020-2024
Universitätsklinikum Knappschaftskrankenhaus Bochum
2020-2024
Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico
2022
University Hospital Magdeburg
2019-2020
Otto-von-Guericke University Magdeburg
2017-2020
University of Duisburg-Essen
2011-2018
Essen University Hospital
2009-2017
Kliniken Essen-Mitte
2013
The prevalence of nonalcoholic steatohepatitis (NASH) associated hepatocellular carcinoma (HCC) is increasing. However, strategies for detection early-stage HCC in patients with NASH have limitations. We assessed the ability GALAD score, which determines risk based on patient sex; age; and serum levels α-fetoprotein (AFP), AFP isoform L3 (AFP-L3), des-gamma-carboxy prothrombin (DCP), to detect NASH.We performed a case-control study 125 (20% within Milan Criteria) 231 without (NASH controls)...
Abstract Nonalcoholic fatty liver disease (NAFLD) is the most common in industrialized countries and may proceed to steatohepatitis (NASH). Apoptosis free acid (FFA)-induced lipotoxicity are important features of NASH pathogenesis. We have shown a hepatoprotective effect adiponectin steatotic livers hepatitis C virus (HCV) patients recent data links bile (BA) metabolism pathogenesis NAFLD. The aim this study was identify potential interactions between BA FFA Liver biopsies serum samples from...
Non-alcoholic fatty liver disease (NAFLD) is closely linked to obesity and constitutes part of the metabolic syndrome, which have been associated with low serum vitamin D (VD). Due known crosstalk between VD transforming growth factor (TGF)-β signalling, has proposed as an antifibrotic treatment.We evaluated association VD, receptor (VDR) fibrosis in primary human hepatic stellate cells (phHSC) 106 morbidly obese patients NAFLD.Treating phHSC ameliorated TGF-β-induced fibrogenesis via both...
OBJECTIVES: The precipitous increase in nonalcoholic steatohepatitis (NASH) is accompanied by a dramatic the incidence of NASH-related hepatocellular carcinoma (HCC). HCC NASH has higher propensity to arise without pre-existing cirrhosis compared with other chronic liver diseases. METHODS: To identify potential links between and gut hepatocarcinogenesis, we microbiota mediators bile acid (BA) signaling absence or presence through analysis stool serum samples from patients non-HCC NASH-HCC...
Upon liver injury, hepatic stellate cells (HSCs) transdifferentiate to migratory, proliferative and extracellular matrix-producing myofibroblasts (e.g., activated HSCs; aHSCs) causing fibrosis. HSC activation is associated with increased glycolysis glutaminolysis. Here, we compared the contribution of glycolysis, glutaminolysis mitochondrial oxidative phosphorylation (OXPHOS) in rat human activation. Basal levels (extracellular acidification rate ~3-fold higher) particularly respiration...
Abstract Objective Progressive hepatic fibrosis can be considered the final stage of chronic liver disease. Hepatic stellate cells (HSC) play a central role in fibrogenesis. Thyroid hormones (TH, e.g. thyroxine; T4 and triiodothyronine; T3) significantly affect development, growth, cell differentiation metabolism through activation TH receptor α and/or β (TRα/β). Here, we evaluated influence Design Human tissue was obtained from explanted livers following transplantation. TRα‐deficient...
Fetuin-A is a pro-inflammatory protein expressed by hepatocytes. Its course in morbidly obese patients with NAFLD (non-alcoholic fatty liver disease) following weight loss BAS (bariatric surgery) has not been fully elucidated yet. In the present study, we prospectively examined effects of on various metabolic factors at 4 weeks and 6 months after surgery. Blood tissues were retrieved from 108 before/during BAS, 50 these individuals met criteria for NASH steatohepatitis). expression was...
Background & Objective Currently, a major clinical challenge is to distinguish between chronic liver disease caused by metabolic syndrome (non-alcoholic fatty disease, NAFLD) from that long term or excessive alcohol consumption (ALD). The etiology of severe affects treatment options and priorities for transplantation organ allocation. Thus we compared physiologically similar NAFLD ALD patients detect biochemical differences improved separation these mechanistically overlapping etiologies....
Abstract Background & Aims Non‐alcoholic fatty liver disease (NAFLD) is characterized by excessive lipid accumulation, inflammation and an imbalanced redox homeostasis. We hypothesized that systemic free thiol levels, as a proxy of oxidative stress, are associated with NAFLD. Methods Protein‐adjusted serum concentrations were determined in participants from the Prevention Renal Vascular End‐Stage Disease (PREVEND) cohort study (n = 5562). Suspected NAFLD was defined Fatty Liver Index...
Cholangiocarcinoma (CCA) cells paradoxically express the death ligand tumor necrosis factor-related apoptosis-inducing (TRAIL) and thus rely on potent survival signals to circumvent cell by TRAIL. Hedgehog (Hh) signaling is an important pathway in CCA. Herein, we further examine mechanisms whereby Hh mediates apoptosis resistance CCA, revealing a pivotal role for division regulating serine/threonine kinase polo-like 2 (PLK2). We employed 50 human CCA samples (25 intrahepatic 25 extrahepatic...
Abstract Krüppel-like factor 6 (KLF6) is a transcription and tumor suppressor. We previously identified KLF6 as mediator of hepatocyte glucose lipid homeostasis. The loss or reduction linked to the progression hepatocellular carcinoma, but its contribution liver regeneration repair in acute injury are lacking so far. Here we explore role models mice, patients with failure (ALF). was induced hepatocytes ALF, both acetaminophen (APAP)- carbon tetrachloride (CCl 4 )-treated mice. In mice...
Hepatic lipid accumulation and mitochondrial dysfunction are hallmarks of metabolic associated fatty liver disease (MAFLD), yet molecular parameters underlying MAFLD progression not well understood. Differential methylation within the DNA (mtDNA) has been suggested to be with dysfunctional mitochondria, also during Metabolic Steatohepatitis (MeSH). This study further investigates whether mtDNA is hepatic MAFLD.HepG2 cells were constructed stably express mitochondria-targeted viral...
Mechanisms that control progression from simple steatosis to steato-hepatitis and fibrosis in patients with non-alcoholic fatty liver disease (NAFLD) are unknown. SPARC, a secreted matricellular protein, is over-expressed the under chronic injury. Contribution of SPARC accumulation severity largely unknown NAFLD. We assessed hypothesis increased livers more necrosis inflammation could be associated fibrosis. qrt-PCR, immunohistochemistry, ELISA were employed localize quantify changes 62...
Binge drinking, i.e., heavy episodic drinking in a short time, has recently become an alarming societal problem with negative health impact. However, the harmful effects of acute alcohol injury gut-liver axis remain elusive. Hence, we focused on physiological and pathological changes underlying mechanisms experimental binge context axis. Eight-week-old mice C57BL/6 background received single dose (p.o.) ethanol (EtOH) [6 g/kg b.w.] as preclinical model injury. Controls PBS. Mice were...
Liver fibrosis is characterized by the accumulation of extracellular matrix proteins, mainly composed collagen. Hepatic stellate cells (HSCs) mediate liver secreting Vitamin C (ascorbic acid) a cofactor prolyl-hydroxylases that modify newly synthesized collagen on route for secretion. Unlike most animals, humans cannot synthesize ascorbic acid and its role in remains unclear. Here, we determined effect prolyl-hydroxylase inhibition production secretion human HSCs. Primary HSCs (p-hHSCs)...
Patients with advanced hepatocellular carcinoma (HCC) arising in nonalcoholic fatty liver disease (NAFLD) may not be suitable for systemic therapy due to metabolic syndrome-related diseases. Recent trials did show a survival benefit of radioembolization (RE) compared sorafenib stage HCC but RE represent an adequate alternative patients contraindications its favorable safety profile.To investigate the impact NAFLD-related comorbidities on and efficacy treatment retrospective monocentric...
Non-alcoholic fatty liver disease (NAFLD) is the hepatic manifestation of metabolic syndrome. Particularly morbidly obese patients are at risk developing progressive disease. Nutritional and lifestyle intervention recommended as standard care in NAFLD. However, there a striking lack evidence to support efficacy treat NAFLD patients. Here, we aimed assess impact on real-world setting.136 were included an industry-independent, multiprofessional program with lead-in phase 12 weeks formula diet...
High-calorie diets lead to hepatic steatosis and the development of non-alcoholic fatty liver disease (NAFLD), which can evolve over many years into inflammatory form steatohepatitis (NASH), posing a risk for hepatocellular carcinoma (HCC). Due diet alteration, axis between gut is disturbed, resulting in microbiome alterations. Consequently, detecting these alterations represents promising strategy early NASH HCC detection. We analyzed medical parameters fecal metaproteome 19 healthy...
Alcoholic liver disease (ALD) and non-alcoholic fatty (NAFLD) have emerged as leading causes of chronic diseases worldwide. ALD NAFLD share several pathophysiological patterns well histological features, while clinically, they are distinguished by the amount alcohol consumed daily. However, coexists with moderate consumption in a growing proportion population. Here, we investigated effects on injury, lipid metabolism, gut microbiota 30 NAFLD-patients. We anonymously assessed drinking habits,...
Introduction Changes in thyroid hormone levels, mostly as non-thyroidal illness syndrome (NTIS), have been described many diseases. However, the relationship between acute liver failure (ALF) and levels has not yet clarified. The present study evaluates potential correlations of select functional parameters with ALF. Methods 84 consecutively recruited ALF patients were grouped according to outcome (spontaneous recovery: SR; transplantation or death: NSR). TSH, free thyroxine (fT4),...
<b><i>Background/Aims:</i></b> Hepatocellular carcinoma (HCC) is the sixth most common malignancy worldwide and therapeutic options are scarce. As they might represent future targets for cancer therapy, expression of apoptosis-related genes in HCC particular interest. In this pilot study, we further examined human also focused on vitamin D signaling as be a regulator cell apoptosis. <b><i>Methods:</i></b> We employed tumor tissue serum samples...