A5068413566- Liver Disease Diagnosis and Treatment
- Liver physiology and pathology
- Liver Disease and Transplantation
- Alcohol Consumption and Health Effects
- Organ Transplantation Techniques and Outcomes
- Pancreatic function and diabetes
- Diet, Metabolism, and Disease
- Phagocytosis and Immune Regulation
- MicroRNA in disease regulation
- Pancreatitis Pathology and Treatment
- Diabetes and associated disorders
- Hepatitis C virus research
- Hormonal Regulation and Hypertension
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Biomarkers in Disease Mechanisms
- Endoplasmic Reticulum Stress and Disease
- Epigenetics and DNA Methylation
- Pediatric Hepatobiliary Diseases and Treatments
- Circular RNAs in diseases
- Spanish Culture and Identity
- Adipokines, Inflammation, and Metabolic Diseases
- Biochemical effects in animals
- Cardiovascular Disease and Adiposity
- Cancer-related molecular mechanisms research
- Peroxisome Proliferator-Activated Receptors
Universitat de Barcelona
2014-2025
Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas
2014-2024
Consorci Institut D'Investigacions Biomediques August Pi I Sunyer
2013-2024
Centre for Biomedical Network Research on Rare Diseases
2024
Fundació Clínic per a la Recerca Biomèdica
2023
Fundació de Recerca Clínic Barcelona-Institut d’Investigacions Biomèdiques August Pi i Sunyer
2023
Hospital General Universitario Gregorio Marañón
2023
Charité - Universitätsmedizin Berlin
2022
Gilead Sciences (United States)
2022
Hospital Clínic de Barcelona
2019-2021
Defining the trajectory of cells during differentiation and disease is key for uncovering mechanisms driving cell fate identity. However, trajectories human remain largely unexplored due to challenges studying them with samples. In this study, we investigate proteome iPSCs hepatic stellate (diHSCs) identify RORA as a transcription factor governing metabolic reprogramming HSCs necessary diHSCs' commitment, identity, activation. Using deficient pharmacologic interventions, show that required...
Alcoholic hepatitis (AH) is a severe condition developed in patients with underlying alcoholic liver disease. Ductular reaction has been associated chronic alcohol consumption but there no information regarding the extent of progenitor cell (LPC) proliferation AH. The aim this study was to investigate LPC markers AH and its correlation disease severity. Fifty-nine clinical histological diagnosis were included study. assessed by real-time polymerase chain (PCR) immunohistochemistry. Standard...
Severe liver diseases are characterized by expansion of progenitor cells (LPC), which correlates with disease severity. However, the origin and role LPC in physiology hepatic injury remains a contentious topic. We found that ductular reaction human cirrhotic livers express hepatocyte nuclear factor 1 homeobox B (HNF1β). HNF1β expression was not present newly generated epithelial cell adhesion molecule (EpCAM)-positive hepatocytes. In order to investigate HNF1β-expressing we used...
Objective Chemokines are known to play an important role in the pathophysiology of alcoholic hepatitis (AH), a form acute-on-chronic liver injury frequently mediated by gut derived lipopolysaccharide (LPS). In our study, we hypothesise that chemokine CCL20, one most upregulated chemokines patients with AH, is implicated pathogenesis AH mediating LPS induced injury. Design CCL20 gene expression and serum levels their correlation disease severity were assessed AH. Cellular sources its...
<h3>Objective</h3> MicroRNAs (miRNAs) are well-known regulators of disease pathogenesis and have great potential as biomarkers therapeutic targets. We aimed at profiling miRNAs in alcoholic hepatitis (AH) identifying potentially involved liver injury. <h3>Design</h3> MiRNA was performed samples from patients with AH, alcohol disease, non-alcoholic steatohepatitis, HCV normal tissue. Expression assessed serum AH animal models. Mimic decoy miR-182 were used vitro vivo to evaluate miR-1829s...
Abstract Unveiling the regulatory pathways maintaining hepatic stellate cells (HSC) in a quiescent (q) phenotype is essential to develop new therapeutic strategies treat fibrogenic diseases. To uncover miRNA-mRNA interactions qHSCs, HSCs were FACS-sorted from healthy livers and activated (aHSCs) generated vitro . MiRNA Taqman array analysis showed expressed low number of miRNAs (n = 259), which 47 down-regulated 212 up-regulated upon activation. Computational integration miRNA gene...
Chronic liver diseases are characterized by the expansion of ductular reaction (DR) cells and expression progenitor cell (LPC) markers. In alcoholic hepatitis (AH), degree DR correlates with disease progression short‐term survival. However, little is known about biological properties cells, their impact on pathogenesis human disease, contribution to tissue repair. this study, we have evaluated transcriptomic profile laser capture microdissection in patients AH assessed its association...
MicroRNA 155 (miR-155) is involved in immune and inflammatory diseases associated with liver fibrosis steatohepatitis. However, the mechanisms miR-155 regulation of injury are largely unknown. The role acute was assessed wild-type (WT), miR-155-/- , mice transplanted WT bone marrow. Additionally, expression evaluated tissue peripheral blood mononuclear cells patients autoimmune hepatitis. Concanavalin A, but not acetaminophen, treatment increased mice. A induced increases cell death,...
// Adil El Taghdouini 1, * , Anita L. Sørensen 2, Andrew H. Reiner 2 Mar Coll 3 Stefaan Verhulst 1 Inge Mannaerts Cristina I. Øie 4 Bård Smedsrød Mustapha Najimi 5 Etienne Sokal Aernout Luttun 6 Pau Sancho-Bru Philippe Collas Leo A. van Grunsven Liver Cell Biology Lab, Vrije Universiteit Brussel (VUB), Brussels, Belgium Department of Molecular medicine, Institute Basic Medical Sciences, Faculty Medicine, University Oslo, Norway Institut d’Investigacions...
Background and AimsGAS6 signaling, through the TAM receptor tyrosine kinases AXL MERTK, participates in chronic liver pathologies. Here, we addressed GAS6/TAM involvement Non-Alcoholic SteatoHepatitis (NASH) development.MethodsGAS6/TAM signaling was analyzed cultured primary hepatocytes, hepatic stellate cells (HSC) Kupffer (KCs). Axl-/-, Mertk-/- wild-type C57BL/6 mice were fed with Chow, High Fat Choline-Deficient Methionine-Restricted (HFD) or methionine-choline-deficient (MCD) diet. HSC...
Background & AimsYearly, approximately 20million people become infected with the hepatitis E virus (HEV) resulting in over 3million cases of acute hepatitis. Although HEV-mediated is usually self-limiting, severe fulminant as well chronic infections have been reported, annually an estimated 60,000 deaths. We studied whether pluripotent stem cell (PSC)-derived hepatocytes, mesodermal and/or neuroprogenitor cells support HEV replication.MethodsHuman PSC were differentiated towards...
The molecular mechanisms driving the progression from early-chronic liver disease (CLD) to cirrhosis and, finally, acute-on-chronic failure (ACLF) are largely unknown. Our aim was develop a protein network-based approach investigate pathways early-CLD ACLF.Transcriptome analysis performed on biopsies patients at different stages, including fibrosis, compensated cirrhosis, decompensated and ACLF, control healthy livers. We created 9 liver-specific disease-related protein-protein interaction...
Fibrosis contributes to 45% of deaths in industrialized nations and is characterized by an abnormal accumulation extracellular matrix (ECM). There are no specific anti-fibrotic treatments for liver fibrosis, previous unsuccessful attempts at drug development have focused on preventing ECM deposition. Because fibrosis largely acknowledged be reversible, regulating resolution could offer novel therapeutical options. However, little known about the mechanisms controlling remodeling during...
Acute‐on‐chronic liver injury is characterized by an important inflammatory response frequently associated with endotoxemia. In this context, acute‐phase proteins such as Pentraxin‐3 (PTX3) are released; however, little known about their role in chronic disease. The aim of study was to elucidate the PTX3 injury. evaluated cultured human cells, tissue slices, and mice acute‐on‐chronic expression assessed serum samples from 54 patients alcoholic hepatitis. up‐regulated animal models strongly...
Background and Aims Bacterial infections are common severe in cirrhosis, but their pathogenesis is poorly understood. Dysfunction of liver macrophages may play a role, information about function cirrhosis limited. Our aims were to investigate the specific profile contribution infections. Macrophages from human cirrhotic livers characterized phenotypically by transcriptome analysis flow cytometry; was assessed vivo single photon emission computerized tomography patients with cirrhosis. Serum...
Ductular reaction (DR) expands in chronic liver diseases and correlates with disease severity. Besides its potential role regeneration, DR plays a the wound-healing response of liver, promoting periductular fibrosis inflammatory cell recruitment. However, there is no information regarding intrahepatic angiogenesis. In current study we investigated contribution cells to hepatic vascular remodeling during disease.In mouse models injury, express genes involved Among angiogenesis-related genes,...
Abstract Fatty-acid-binding proteins (FABPs) are small intracellular that coordinate lipid-mediated processes by targeting metabolic and immune response pathways. The aim of the study was to investigate plasma FABPs levels their relationship with clinical outcomes in cirrhosis. Plasma L-FABP1(liver kidney), I-FABP2(intestine), A-FABP4(adipocyte macrophages) were measured 274 patients decompensated Hepatic gene expression assessed liver biopsies from cirrhosis cell types mice...
MicroRNAs (miRNAs) circulate in several body fluids and can be useful biomarkers. The aim of this study was to identify blood-circulating miRNAs associated with cirrhosis progression acute-on-chronic liver failure (ACLF).Using high-throughput screening 754 miRNAs, serum samples from 45 patients compensated cirrhosis, decompensated or ACLF were compared those healthy individuals (n = 15). miRNA levels correlated clinical parameters, organ failure, disease outcome. Dysregulated evaluated...
: The accumulation of adipose tissue macrophages (ATMs) in obesity has been associated with hepatic injury. However, the contribution ATMs to fibrosis non-alcoholic fatty liver disease (NAFLD) remains be elucidated. Herein, we investigate relationship between and patients NAFLD evaluate impact modulation over an experimental steatohepatitis (NASH) model.Adipose biopsies from 42 different stages were collected. characterised by immunohistochemistry flow cytometry correlation was assessed....