A5028055383- Pharmacogenetics and Drug Metabolism
- Liver physiology and pathology
- Drug Transport and Resistance Mechanisms
- Computational Drug Discovery Methods
- Drug-Induced Hepatotoxicity and Protection
- 3D Printing in Biomedical Research
- Pancreatic function and diabetes
- Carcinogens and Genotoxicity Assessment
- Animal testing and alternatives
- Liver Disease Diagnosis and Treatment
- Organ Transplantation Techniques and Outcomes
- Molecular Biology Techniques and Applications
- Statistical Methods in Clinical Trials
- Viral Infectious Diseases and Gene Expression in Insects
- Pharmacological Effects and Toxicity Studies
- Barrier Structure and Function Studies
- Dermatologic Treatments and Research
- Liver Disease and Transplantation
- Hepatocellular Carcinoma Treatment and Prognosis
- Pluripotent Stem Cells Research
- RNA Interference and Gene Delivery
- Contact Dermatitis and Allergies
- Synthesis and biological activity
- Skin and Cellular Biology Research
- Advancements in Transdermal Drug Delivery
Biopredic (France)
2015-2024
Centre Hospitalier privé Saint Grégoire
2010-2023
Inserm
1988-1999
Uppsala University
1996
Hôpital Pontchaillou
1988-1994
HepaRG cells possess the unique property to differentiate in vitro and express various functions of mature hepatocytes, including major cytochromes P450 (P450s). In present study, we carefully analyzed mRNA expression activity P450s their responsiveness three prototypical inducers, phenobarbital, rifampicin, omeprazole, differentiated cell cultures over a 4-week period after low high seeding. Only minor differences were observed activities when measured by two cocktails probe substrates,...
Striae distensae evaluation criteria have been recently described, but none is focused on objective striae assessment. With the purpose of better and objectively estimating severity distensae, Objective Stretch Marks Assessment Scale has developed by authors' team.Seven hundred White patients were included in study assessed. To assess abdomen, breasts, hips, gluteal area, back thighs, calves, upper limbs photonumeric grading scales developed. The Rasch model was used as part validation...
Cryopreserved hepatocytes from various animal species and human beings were tested for their ability to survive function in primary culture. The freeze/thaw protocol primarily designed rat was used with slight modifications the cells of all other species; it consisted suspending parenchymal Leibovitz L15 medium containing 10% fetal calf serum 16% dimethyl sulfoxide. After transient storage at 4 degrees C cell suspensions transferred -20 then -70 before being plunged liquid nitrogen....
The aim of this work was to conduct a comprehensive study about the transport properties NSAIDs across blood-brain barrier (BBB) in vitro. Transport studies with celecoxib, diclofenac, ibuprofen, meloxicam, piroxicam and tenoxicam were accomplished Transwell models based on cell line PBMEC/C1-2, ECV304 or primary rat brain endothelial cells. Single as well group substance carried out. In compositions, medium serum content varied, inhibitors verapamil probenecid added. Resulted permeability...
1. The metabolism of Meloxicam (ME) and the cytochrome(s) P450 (CYPs) involved were analysed by using primary human hepatocytes, liver microsomes from recombinant B-lymphoblastoid cell lines. 2. While hepatocytes capable converting ME to a 5-hydroxymethyl metabolite (M7) then 5-carboxyderivative (M5), formed mostly only 5-hydroxymethylderivative. kinetics formation M7 biphasic with Km = 13.6 +/- 9.5 381 55.2 microM respectively. corresponding Vmax 33.7 24.2 143 83.9 pmol/min/mg protein 3....
Recent studies have demonstrated that various DNA adducts can be detected in human tissues and fluids using liquid chromatography connected to tandem mass spectrometry (LC-MS/MS). However, the utility of a single adduct as biomarker risk assessment is debatable because humans are exposed many genotoxicants. We established method measure derived from 16 ubiquitous genotoxicants developed an analytical technique for their simultaneous quantification by ultra performance (UPLC)-MS/MS. Methods...
Genetic toxicity information is critical for the safety assessment of all xenobiotics. In absence carcinogenicity data, genetic studies may be used to draw conclusions about potential chemicals. However, current in vitro assays have many limitations as they produce a high rate irrelevant positive data and possible false negative due weakness models used. Based on knowledge that majority human genotoxic carcinogens require metabolic activation become genotoxic, it necessary develop cell mimic...
The hepatoma-derived cell line HepaRG is regarded as an in vitro model of drug metabolism because fully differentiated cells demonstrate functional metabolic responses comparable to those primary human hepatocytes. Recently, it was demonstrated that the 3D culture enhanced their functions and toxicological responses. We approached mechanisms underlying these enhancement effects.We compared 2D-cultured with 3D-cultured spheroids gene expression patterns functions. In present study, we...
Despite decades of investigation on the proliferation adult human primary hepatocytes, their expansion in vitro still remains challenging. To later be able to consider hepatocytes as a cell therapy alternative or bridge liver transplantation, dramatically impeded by shortage donors, first step is having an almost unlimited source these cells. The banking transplantable also implies protocol for that can compatible with large-scale production. We show when grown 3D organoids are easily...
CYP enzyme induction is a sensitive biomarker for phenotypic metabolic competence of in vitro test systems; it key event associated with thyroid disruption, and toxicologically relevant nuclear receptor-mediated pathways. This paper summarises the results multi-laboratory validation study two methods that assess potential chemicals to induce cytochrome P450 (CYP) activity, particular CYP1A2, CYP2B6, CYP3A4. The are based on use cryopreserved primary human hepatocytes (PHH) HepaRG cells. was...
The purpose of this project report is to introduce the European “GOLIATH” project, a new research which addresses one most urgent regulatory needs in testing endocrine-disrupting chemicals (EDCs), namely lack methods for EDCs that disrupt metabolism and metabolic functions. These collectively referred as “metabolism disrupting compounds” (MDCs) are natural anthropogenic can promote changes ultimately result obesity, diabetes, and/or fatty liver humans. This introduces main approaches...
Results from tests of the first 30 MEIC reference chemicals in 68 different toxicity assays are presented as a prerequisite to subsequent vitro/in vivo comparisons acute data. A comparative cytotoxicity study was also carried out. Firstly, variability all results analysed by using principal components analysis (PCA), analyses variance (ANOVAs) and pairwise means according Tukey's method. The PCA component described 80% ANOVA indicated similar sensitivity for assays, approximately 80%....
As the conventional approach to assess potential of a chemical cause cancer in humans still includes 2-year rodent carcinogenicity bioassay, development alternative methodologies is needed. In present study, transcriptomics responses following exposure genotoxic (GTX) and non-genotoxic (NGTX) hepatocarcinogens non-carcinogens (NC) five liver-based vitro models, namely epigenetically stabilized cultures primary rat hepatocytes, human hepatoma-derived cell lines HepaRG HepG2 embryonic stem...
HepaRG cells have the ability to differentiate into hepatocyte-like cells. Many papers shown that these share several functional properties with intact human hepatocytes. However, although previous studies indicated partial maintenance of mRNA expression drug transporters, their and function not been quantitatively characterized. In present study, protein levels transport activities hepatic uptake organic anion transporting polypeptides (OATPs) Na+-taurocholate cotransporting polypeptide...