A5074195161- Polysaccharides and Plant Cell Walls
- Polysaccharides Composition and Applications
- Enzyme Production and Characterization
- Microbial Metabolic Engineering and Bioproduction
- Microbial Metabolites in Food Biotechnology
- Bacterial Genetics and Biotechnology
- Analytical Methods in Pharmaceuticals
- Enzyme Catalysis and Immobilization
- CRISPR and Genetic Engineering
- Analytical Chemistry and Chromatography
- Glycosylation and Glycoproteins Research
- Stability and Controllability of Differential Equations
- Biofuel production and bioconversion
- Genomics and Phylogenetic Studies
- Advanced Mathematical Modeling in Engineering
- Mass Spectrometry Techniques and Applications
- Pharmacogenetics and Drug Metabolism
- RNA and protein synthesis mechanisms
- Carbohydrate Chemistry and Synthesis
- Antibiotics Pharmacokinetics and Efficacy
- Pharmacological Effects and Assays
- Seaweed-derived Bioactive Compounds
- Receptor Mechanisms and Signaling
- Plant pathogens and resistance mechanisms
- Manufacturing Process and Optimization
University of Münster
2020-2024
University of Basel
2024
German Center for Infection Research
2024
Technical University of Munich
2012-2023
Fraunhofer Institute for Industrial Mathematics
2023
Universitätsklinikum Erlangen
2022
Norwegian University of Science and Technology
2019-2022
Stadtwerke Straubing (Germany)
2016-2019
Biogen (Germany)
2011-2017
Bucharest University of Economic Studies
2015-2017
The enzyme subclass of glycosyltransferases (GTs; EC 2.4) currently comprises 97 families as specified by CAZy classification. One their important roles is in the biosynthesis disaccharides, oligosaccharides, and polysaccharides catalyzing transfer sugar moieties from activated donor molecules to other molecules. In addition GTs also catalyze onto aglycons, which great relevance for synthesis many high value natural products. Bacterial show a higher sequence similarity comparison mammalian...
The study was conducted in healthy male volunteers to evaluate the absorption, metabolic pattern, and mode of elimination telmisartan, a nonpeptide angiotensin II receptor antagonist. [ 14 C]telmisartan administered orally solution as single 40 mg dose 5 subjects. A further subjects received short‐term intravenous infusion mg. Measurement total C radioactivity plasma showed that about 50% absorbed following oral administration, with maximum concentration observed after 0.5 1 hour. Absolute...
Abstract Antamanide is the name we have given to a constituent of fungus Amanita phalloides. This substance counteracts lethal action toxins phalloidine and α‐amanatine if administered white mouse before, or simultaneously with, poisons. Its concentration in is, however, so low that toxic latter predominates. cyclic decapeptide formed from L ‐amino acids alanine, phenylalanine, proline, valine molar ratio 1:4:4:1. The amino‐acid sequence was determined by combination gas chromatography mass...
Abstract Als Antamanid bezeichnen wir einen Inhaltsstoff des grünen Knollenblätterpilzes, Amanita phalloides, der an weißen Maus die tödliche Wirkung Amanitatoxine Phalloidin und α‐Amanitin bei spätestens gleichzeitiger Verabreichung aufhebt. Die Konzentration im Pilz ist allerdings so gering, daß dessen Giftwirkung überwiegt. ein cyclisches Dekapeptid aus den L‐Aminosäuren Alanin, Phenylalanin, Prolin Valin, zueinander Molverhältnis von 1:4:4:1 stehen. Sequenz wurde durch...
1. The metabolism of Meloxicam (ME) and the cytochrome(s) P450 (CYPs) involved were analysed by using primary human hepatocytes, liver microsomes from recombinant B-lymphoblastoid cell lines. 2. While hepatocytes capable converting ME to a 5-hydroxymethyl metabolite (M7) then 5-carboxyderivative (M5), formed mostly only 5-hydroxymethylderivative. kinetics formation M7 biphasic with Km = 13.6 +/- 9.5 381 55.2 microM respectively. corresponding Vmax 33.7 24.2 143 83.9 pmol/min/mg protein 3....
Application of state-of-the-art genome editing tools like CRISPR-Cas9 drastically increase the number undomesticated micro-organisms amenable to highly efficient and rapid genetic engineering. Adaptation these new bacterial families can open up entirely possibilities for organisms accelerate as biotechnologically relevant microbial factories, also making products economically competitive. Here, we report implementation a based vector system in Paenibacillus polymyxa, enabling fast reliable...
Microbial exopolysaccharides (EPS) are multifunctional biogenic polymers, which exist in highly diverse chemical structures. To facilitate a fast determination of the carbohydrate composition novel isolated strains or modified EPS variants screening and analytical method is required. The platform as realized described this article based on analysis via liquid chromatography coupled with ultra violet electrospray ionization ion trap detection 96-well format to detect different sugars, sugar...
Paenibacillus polymyxa is a Gram-positive, non-pathogenic soil bacterium that has been extensively investigated for the production of R-,R-2,3-butanediol in exceptionally high enantiomeric purity. Rational metabolic engineering efforts to increase productivity and product titers were restricted due limited genetic accessibility organism up now. By use CRISPR-Cas9 mediated genome editing, six mutant variants generated compared batch fermentations first time. Downstream processing was...
Abstract Purpose The objective examination of the Post-COVID syndrome (PCS) remains difficult due to heterogeneous definitions and clinical phenotypes. aim study was verify functionality correlates a recently developed PCS score. Methods score applied prospective, multi-center cross-sectoral cohort (in- outpatients with SARS-CoV-2 infection) "National Pandemic Cohort Network (NAPKON, Germany)". Symptom assessment patient-reported outcome measure questionnaires were analyzed at 3 12 months...
Meloxicam [4-hydroxy-2-methyl-N-(5-methyl-2-thiazolyl)-2H- 1,2-benzothiazine-3-carboxamide-1,1-dioxide] is a new nonsteroidal antiinflammatory drug belonging to the enolic acid group. In crossover study, 30 mg 14C-labeled meloxicam was administered four male healthy volunteers as short-term infusion and an oral solution. The objectives of study were determine mode elimination, excretion balance, in vivo binding characteristics serum proteins, investigate metabolic pattern plasma, urine,...
In analyzing the reductive power of Escherichia coli K-12 for metabolic engineering approaches, we identified YahK and YjgB, two medium-chain dehydrogenases/reductases subgrouped to cinnamyl alcohol dehydrogenase family, as being important. Identification was achieved using a stepwise purification protocol starting with crude extract. For exact characterization, genes were cloned into pET28a vector expressed N-terminal His tag. Substrate specificity studies revealed that large variety...