A5100452678- Inflammasome and immune disorders
- Alcohol Consumption and Health Effects
- Cancer Mechanisms and Therapy
- Heme Oxygenase-1 and Carbon Monoxide
- Tea Polyphenols and Effects
- interferon and immune responses
- Immune Cell Function and Interaction
- Liver Disease Diagnosis and Treatment
- T-cell and B-cell Immunology
- Adipose Tissue and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- RNA modifications and cancer
- Galectins and Cancer Biology
- Immunotherapy and Immune Responses
- Nuclear Structure and Function
- Cancer Immunotherapy and Biomarkers
- Cancer, Hypoxia, and Metabolism
- Vitamin D Research Studies
- Peroxisome Proliferator-Activated Receptors
- Gout, Hyperuricemia, Uric Acid
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- Systemic Lupus Erythematosus Research
- Immune cells in cancer
- Extracellular vesicles in disease
Cleveland Clinic Lerner College of Medicine
2017-2025
Case Western Reserve University
2020-2025
Obstetrics and Gynecology Hospital of Fudan University
2025
Tohoku University
2023-2024
Anhui Medical University
2022-2024
Chongqing Medical University
2017-2024
Zhejiang Hospital
2024
Dalian Medical University
2024
The Affiliated Yongchuan Hospital of Chongqing Medical University
2017-2024
Ministry of Education of the People's Republic of China
2023
5-Fluorouracil (5-FU) is a widely used drug for the therapy of cancer. However, chemoresistance tumor cells to 5-FU usually limits its clinical effectiveness. In this study, we explored role NLRP3 inflammasome in resistance oral squamous cell carcinoma (OSCC). The mRNA and protein expression levels NLRP3, Caspase1 IL-1β resected OSCC specimens or lines were measured respectively by quantitative real time-PCR (qRT-PCR) western blot. Ki-67 paraffin-embedded tissues was determined...
Gasdermin D (GSDMD) induces pyroptosis via the pore-forming activity of its N-terminal domain, cleaved by activated caspases associated with release IL-1β. Here, we report a nonpyroptotic role full-length GSDMD in guiding IL-1β-containing small extracellular vesicles (sEVs) from intestinal epithelial cells (IECs). In response to caspase-8 inflammasome activation, GSDMD, chaperoned Cdc37/Hsp90, recruits E3 ligase, NEDD4, catalyze polyubiquitination pro-IL-1β, serving as signal for cargo...
Inflammasomes are reported to be abnormally expressed and activated in several malignancies play important roles tumor development. The present study was designed investigate the expression function of NLR family pyrin domain containing protein 3 (NLRP3) inflammasome oral squamous cell carcinoma (OSCC). NLRP3 OSCC lines normal human immortalized epithelial cells (HIOEC) determined by real-time PCR western blot. Immunohistochemistry used examine IL-1β paraffin-embedded tissues. proliferation...
Increasing evidence suggests that intratumoral inflammation has an outsized influence on antitumor immunity. Here, we report IL-17, a proinflammatory cytokine widely associated with poor prognosis in solid tumors, drives the therapeutic failure of anti-PD-L1. By timing deletion IL-17 signaling specifically cancer-associated fibroblasts (CAFs) late-stage show immune exclusion by activating collagen deposition program murine models cutaneous squamous cell carcinoma (cSCC). Ablation CAFs...
Inflammation plays important roles at different stages of diabetes mellitus, tumorigenesis and cardiovascular diseases. (−)-Epigallocatechin gallate (EGCG) can attenuate inflammatory responses effectively. However, the immediate early mechanism EGCG in inflammation remains unclear. Here, we showed that attenuated response stage treatment by shutting off Notch signaling, effect did not involve 67-kD laminin receptor (67LR), common for EGCG. eliminated mature from cell membrane nuclear...
CCL24 is one chemotactic factor extensively studied in airway inflammation and colorectal cancer but less hepatocellular carcinoma (HCC) retrospectively. So HCC tissue microarray (TMA) was used to estimate relationship between prognosis, cell experiments were conducted study its influence for biological behavior. injected nude mice monitor tumor formation pulmonary metastasis; qRT-PCR, western blot Immunohistochemistry explore potential mechanism. plays roles target cells via downstream...
Abstract Although Act1 (adaptor for IL-17 receptors) is necessary IL-17-mediated inflammatory responses, - (but not Il17ra- , Il17rc- or Il17rb -) deficient mice develop spontaneous SLE- and Sjögren’s-like diseases. Here, we show that functions as a negative regulator in T B cells via direct inhibition of STAT3. Mass spectrometry analysis detected an Act1–STAT3 complex, deficiency Il17ra -, ) results hyper IL-23- IL-21-induced STAT3 activation cells, respectively. IL-23R deletion blockade...
IL-1β can exit the cytosol as an exosomal cargo following inflammasome activation in intestinal epithelial cells (IECs) a Gasdermin D (GSDMD)-dependent manner. The mechanistic connection linking and biogenesis of exosomes has so far remained largely elusive. Here, we report Ras GTPase-activating-like protein IQGAP1 functions adaptor, bridging GSDMD to endosomal sorting complexes required for transport (ESCRT) machinery promote pro-IL-1β-containing response NLPR3 activation. We identified...
The C-type lectin receptor Mincle is known for its important role in innate immune cells recognizing pathogen and damage associated molecular patterns. Here we report a T cell-intrinsic the pathogenesis of experimental autoimmune encephalomyelitis (EAE). Genomic deletion impairs TH17, but not TH1 cell-mediated EAE, alignment with significantly higher expression TH17 than cells. Mechanistically, dying release β-glucosylceramide during inflammation, which serves as natural ligand Mincle....
Natural killer (NK) cells are important component of innate immunity and also contribute to activating reshaping the adaptive immune responses. The functions NK modulated by multiple inhibitory stimulatory receptors. Among these receptors, receptor CD226 (DNAM-1) mediates cell activation via binding its nectin-like (Necl) family ligand, CD155 (Necl-5). Here, we present a unique side-by-side arrangement pattern two tandem immunoglobulin V-set (IgV) domains deriving from ectodomains both human...
The tea catechin epigallocatechin-3-gallate (EGCG) proved to be the most potent physiologically active compound in vitro. It possesses antioxidant as well pro-oxidant properties. EGCG has effect of inducing apoptosis tumor cells and inhibiting cell proliferation. Whether this is associated with or pro-oxidative effects affecting genome stability normal cancer not been confirmed. Here, we selected Human colon epithelial NCM460 adenocarcinoma COLO205 investigate (0−40 μg/mL) on growth status....
Toll-like receptors/Interleukin-1 receptor signaling plays an important role in high-fat diet-induced adipose tissue dysfunction contributing to obesity-associated metabolic syndromes. Here, we show unconventional IL-1R-IRAKM-Slc25a1 axis adipocytes that reprograms lipogenesis promote obesity. Adipocyte-specific deficiency of IRAKM reduces body weight gain, increases whole energy expenditure and improves insulin resistance, associated with decreased lipid accumulation adipocyte cell sizes....
Background: Macrophage-inducible C-type lectin (Mincle) is expressed on hepatic macrophages and senses ethanol (EtOH)-induced danger signals released from dying hepatocytes promotes IL-1β production. However, it remains unclear what how EtOH-induced Mincle ligands activate downstream signaling events to mediate release contribute alcohol-associated liver disease (ALD). In this study, we investigated the association of circulating β-glucosylceramide (β-GluCer), an endogenous ligand, with...
Expression of inflammatory genes is determined in part by post-transcriptional regulation mRNA metabolism but how stimulus- and transcript-dependent nuclear export influence poorly understood. Here, we report a novel pathway which LPS/TLR4 engagement promotes localization IRAK2 to facilitate specific subset inflammation-related mRNAs for translation murine macrophages. kinase activity required LPS-induced RanBP2-mediated sumoylation subsequent translocation. Array analysis showed that an...
Abstract Background As an immune enhancer, Nocardia rubra cell‐wall skeleton (Nr‐CWS) has been used to treat persistent human papillomavirus infection and cervical precancerous lesions. However, it is still unclear whether can be carcinoma. Methods In our study, the aim was determine Nr‐CWS affects apoptosis of carcinoma cells by enhancing antitumor effect dendritic macrophages in vivo vitro. Results The experimental results showed that promote activity reduce their apoptosis. It also...
Abstract Klf5, a member of the Krüppel-like transcription factor family, has essential roles during embryonic development, cell proliferation, differentiation, migration and apoptosis. This study was to define molecular mechanism Klf5 odontoblastic differentiation. The expression odontoblast-differentiation markers, Dspp Dmp1 co-localized in cells at different stages mouse tooth development dental papilla mesenchymal cells. able promote differentiation enhance mineral formation Furthermore,...
A Gram-stain-positive, aerobic actinobacterium, designated strain NEAU-24T, was isolated from saline-alkali soil collected Daqing City, Heilongjiang Province, PR China. Strain NEAU-24T found to produce abundant substrate mycelia but no aerial hyphae. The formed irregular pseudosporangia consisting of nuciform spores, and the surface spores smooth. 16S rRNA gene sequence analysis showed that clustered with Pseudosporangium ferrugineum 3-44-a(19)T, Couchioplanes caeruleus subsp. azureus DSM...
The surface properties and dissolution kinetics of tea polyphenols, TP, with different polyphenol percents, e.g. at about 80, 90, 100%, were studied. free energy TP was found in the range 39–44 mN/m directly proportional to percent, dominated by Lifshitz–van der Waals interaction component. is strong Lewis base weak acid, acid–base component increased percent increase. water can be enhanced increasing both temperature, this process follows second-order model. DMF has been unlike due ignoring variety.