A5018304322- Ion channel regulation and function
- Cardiac electrophysiology and arrhythmias
- Plant Stress Responses and Tolerance
- Computational Drug Discovery Methods
- Ion Channels and Receptors
- Neuroscience and Neuropharmacology Research
- Nicotinic Acetylcholine Receptors Study
- Electrochemical Analysis and Applications
- Plant Molecular Biology Research
- Animal Virus Infections Studies
- Alzheimer's disease research and treatments
- Receptor Mechanisms and Signaling
- Connexins and lens biology
- Cholinesterase and Neurodegenerative Diseases
- Lipid Membrane Structure and Behavior
- Plant and Biological Electrophysiology Studies
- Virus-based gene therapy research
- Photosynthetic Processes and Mechanisms
- Hydrology and Watershed Management Studies
- Viral gastroenteritis research and epidemiology
- Atrial Fibrillation Management and Outcomes
- Venomous Animal Envenomation and Studies
- Protein Structure and Dynamics
- Mass Spectrometry Techniques and Applications
- Plant nutrient uptake and metabolism
University of Talca
2016-2025
Millennium Nucleus of Ion Channel Associated Diseases
2017-2025
Rhode Island Hospital
2025
Brown University
2018-2025
University of Chile
2020-2025
Universidad Autónoma de Santo Domingo
2022-2023
Karlsruhe Institute of Technology
2021-2022
Centro de Investigación en Red en Enfermedades Cardiovasculares
2020
Instituto de Salud Carlos III
2020
Millennium Engineering and Integration (United States)
2018
The essential mineral nutrient potassium (K + ) is the most important inorganic cation for plants and recognized as a limiting factor crop yield quality. Nonetheless, it only partially understood how K contributes to plant productivity. used major active solute maintain turgor drive irreversible reversible changes in cell volume. also plays an role numerous metabolic processes, example, by serving cofactor of enzymes. Here, we provide evidence additional, previously unrecognized growth. By...
Reactive oxygen species (ROS) are essential for development and stress signaling in plants. They contribute to plant defense against pathogens, regulate stomatal transpiration, influence nutrient uptake partitioning. Although both Ca(2+) K(+) channels of plants known be affected, virtually nothing is the targets ROS at a molecular level. Here we report that single cysteine (Cys) residue within Kv-like SKOR channel Arabidopsis thaliana sensitivity H(2)O(2). We show H(2)O(2) rapidly enhanced...
A key to potassium channel activation Using drugs activate channels has the potential treat conditions like epilepsy, heart arrhythmias, and pain. Schewe et al. report a class of negatively charged activators (NCAs) with defined pharmacore that use similar mechanism many types channels. X-ray crystallography, functional analysis, molecular dynamics simulations showed NCAs bind below selectivity filter open gate Targeting this NCA site might be exploited in rational drug design. Science , issue p. 875
Research Article27 February 2017Open Access Transparent process Sodium permeable and "hypersensitive" TREK-1 channels cause ventricular tachycardia Niels Decher Corresponding Author [email protected] orcid.org/0000-0001-8892-1231 Institute of Physiology Pathophysiology, Vegetative Physiology, Philipps-University Marburg, Germany Search for more papers by this author Beatriz Ortiz-Bonnin Corinna Friedrich Department Cardiovascular Medicine, Genetics Heart Diseases (IfGH), University Hospital...
Potassium channels share a common selectivity filter that determines the conduction characteristics of pore. Diversity in K+ is given by how they are gated open. TASK-2, TALK-1, and TALK-2 two-pore region (2P) KCNK open extracellular alkalinization. We have explored mechanism for this alkalinization-dependent gating using molecular simulation site-directed mutagenesis followed functional assay. show side chain single arginine residue (R224) near pore senses pH TASK-2 with an unusual pKa 8.0,...
Abstract. Numerical models are an important tool for simulating temperature, hydrodynamics, and water quality in lakes reservoirs. Existing differ dimensionality by considering spatial variations of simulated parameters (e.g., flow velocity temperature) one (1D), two (2D) or three (3D) dimensions. The different approaches based on levels simplification the description hydrodynamic processes result demands computational power. aim this study is to compare with dimensionalities analyze...
Atrial fibrillation (AF) involves electrical remodeling of the atria, with ion channels such as NaV1.5, KV1.5, and TASK-1 playing crucial roles. This study investigates acetamide-based compounds designed multi-target inhibitors these to address AF. Compound 6f emerged most potent in series, demonstrating a strong inhibition (IC50 ∼0.3 μM), moderate NaV1.5 ∼21.2 μM) subtle KV1.5 ∼81.5 alongside unexpected activation TASK-4 (∼40% at 100 μM). Functional assays on human atrial cardiomyocytes...
Abstract Background Spinocerebellar ataxia 19/22 (SCA19/22) represents a rare autosomal dominant genetic disorder resulting in progressive and cerebellar atrophy. SCA19/22 is caused by variants the KCND3 gene, which encodes voltage-gated potassium channel subunit essential for Purkinje cell function. To date, 22 have been reported worldwide, with incomplete functional studies. Results We present four Chilean Mexican cases whom two single-nucleotide were identified through whole-exome...
TASK-2 (K2P5.1) is a two-pore domain K(+) channel belonging to the TALK subgroup of K2P family proteins. has been shown be activated by extra- and intracellular alkalinization. Extra- pH-sensors reside at arginine 224 lysine 245 might affect separate selectivity filter inner gates respectively. modulated changes in cell volume regulation direct G-protein interaction also proposed. Activation extracellular alkalinization associated with role kidney proximal tubule bicarbonate reabsorption,...
Snake venoms are rich and intriguing sources of biologically-active molecules that act on target cells, modulating a diversity physiological functions presenting promising pharmacological applications. Lys49 phospholipase A2 is one the multifunctional proteins present in these complex secretions and, although catalytically inactive, has variety biological activities, including cytotoxic, antibacterial, inflammatory, antifungal activities. Herein, A2, denominated CoaTx-II from Crotalus...
Transient receptor potential melastatin 4 (TRPM4) is a Ca2+-activated nonselective cationic channel that regulates cell migration and contractility. Increased TRPM4 expression has been related to pathologies, in which cytoskeletal rearrangement are altered, such as metastatic cancer. Here, we identify the K+ tetramerization domain 5 (KCTD5) protein, putative adaptor of cullin3 E3 ubiquitin ligase, novel TRPM4-interacting protein. We demonstrate KCTD5 positive regulator activity by enhancing...
Withaferin A (WFA), a C5,C6-epoxy steroidal lactone isolated from the medicinal plant Withania somnifera (L.) Dunal, inhibits growth of tumor cells in different cancer types. However, mechanisms underlying effect WFA on are not fully understood. In present study, we evaluated blockade TASK-3 channels by TASK-3-expressing HEK-293 cells. Explore if WFA-mediated can be used as pharmacological tool to decrease cell viability combination functional experiments (patch-clamp, gene downregulation,...