A5005200259- Algal biology and biofuel production
- Photosynthetic Processes and Mechanisms
- Plant Molecular Biology Research
- Chromosomal and Genetic Variations
- Postharvest Quality and Shelf Life Management
- Microbial Inactivation Methods
- Biocrusts and Microbial Ecology
- 3D Printing in Biomedical Research
- Electrochemical sensors and biosensors
- Gene Regulatory Network Analysis
- Viral Infectious Diseases and Gene Expression in Insects
- Fermentation and Sensory Analysis
- Plant Reproductive Biology
- Plant tissue culture and regeneration
- Microbial Metabolic Engineering and Bioproduction
- Light effects on plants
- Photoreceptor and optogenetics research
- Water Quality Monitoring and Analysis
- Plant Stress Responses and Tolerance
- Analytical Chemistry and Sensors
- Single-cell and spatial transcriptomics
- Biochemical Analysis and Sensing Techniques
- Spectroscopy and Chemometric Analyses
- Plant nutrient uptake and metabolism
- Phytochemicals and Antioxidant Activities
Neubrandenburg University of Applied Sciences
2020-2024
Leibniz Institute of Plant Genetics and Crop Plant Research
2013-2021
Institute for Food and Environmental Research
2016-2020
University of Potsdam
2010-2015
Leibniz Association
2014
Interactive Motion Technologies (United States)
2004
Abstract Background Previous results with the planar robot MIT-MANUS demonstrated positive benefits in trials over 250 stroke patients. Consistent motor learning, effects did not generalize to other muscle groups or limb segments. Therefore we are designing a new class of robots exercise This paper presents basic engineering aspects novel robotic module that extends our approach anti-gravity movements out horizontal plane and pilot study 10 outpatients. Patients were trained during initial...
The essential mineral nutrient potassium (K + ) is the most important inorganic cation for plants and recognized as a limiting factor crop yield quality. Nonetheless, it only partially understood how K contributes to plant productivity. used major active solute maintain turgor drive irreversible reversible changes in cell volume. also plays an role numerous metabolic processes, example, by serving cofactor of enzymes. Here, we provide evidence additional, previously unrecognized growth. By...
We applied a top-down systems biology approach to understand how Chlamydomonas reinhardtii acclimates long-term heat stress (HS) and recovers from it. For this, we shifted cells 25 42°C for 24 h back 25°C ≥8 monitored abundances of 1856 proteins/protein groups, 99 polar 185 lipophilic metabolites, cytological photosynthesis parameters. Our data indicate that acclimation HS consists temporally ordered, orchestrated implementation response elements at various system levels. These comprise (1)...
The centromeric histone H3 variant cenH3 is an essential protein required for assembly, maintenance, and proper function of kinetochores during mitosis meiosis. We identified a kinetochore null2 (KNL2) homolog in Arabidopsis thaliana uncovered features its role loading at centromeres. show that KNL2 colocalizes with associated centromeres all stages the mitotic cell cycle, except from metaphase to mid-anaphase. regulated by proteasome degradation pathway. promoter mainly active meristematic...
KINETOCHORE NULL2 (KNL2) is involved in recognition of centromeres and centromeric localization the centromere-specific histone cenH3. Our study revealed a cenH3 nucleosome binding CENPC-k motif at C terminus Arabidopsis thaliana KNL2, which conserved among wide spectrum eukaryotes. Centromeric KNL2 abolished by deletion mutating single amino acids, but can be restored insertion corresponding CENP-C. We showed electrophoretic mobility shift assay that binds DNA sequence-independently...
Potassium (K+) is an important nutrient for plants. It serves as a cofactor of various enzymes and the major inorganic solute maintaining plant cell turgor. In recent study, yet unknown role K+ in homeostasis was shown. demonstrated that gradients vascular tissues can serve energy source phloem (re)loading processes voltage-gated channels AKT2-type play unique this process. The AKT2 channel be converted by phosphorylation specific serine residues (S210 S329) into non-rectifying allows rapid...
Microalgae, and particularly the cyanobacterium Arthrospira platensis (spirulina), have attracted much attention due to their wide range of uses. The potential use spirulina in food is mainly driven by its high content macro micronutrients including proteins, γ-linolenic acid, sulfated polysaccharides, minerals, vitamins, natural pigment phycocyanin. Despite these benefits, still not widely used industry numerous technological challenges during manufacturing or specific sensory issues final...
Abstract Objective Flow cytometry (FC) is probably the most important technique for single-cell analysis. It’s precisely, rapid, and suitable multidimensional The commonly used side scatter (SSC) intensity determined by FC often interpreted as a measure of internal cellular complexity cells. In simple terms, more structured cell is, higher SSC quantified FC. Nevertheless, studies that support this interpretation are based on data derived from animal or human lines while it assumed results...
Microalgae bear a great potential to produce lipids for biodiesel, feed, or even food applications. To understand the still not well-known single-cell dynamics during lipid production in microalgae, novel analytical technology was applied study well-established model experiment. Multidimensional were investigated with non-supervised image analysis technique that utilizes data from epi-fluorescence microscopy. Reliability of this successfully proven via reference analysis. The developed used...
Due to multiple light scattering that occurs inside and between cells, quantitative optical spectroscopy in turbid biological suspensions is still a major challenge. This includes also inline determination of biomass bioprocessing. Photon Density Wave (PDW) spectroscopy, technique based on scattering, enables the independent absolute key parameters concentrated cell suspensions, which allow determine during cultivation.A unique reactor type, called "mesh ultra-thin layer photobioreactor" was...
While the treatment of red grapes with pulsed electric fields (PEFs) has been widely investigated, white have little studied to date. This work, therefore, investigates PEF (Thompson Seedless) by applying different field strengths (2 6 kV/cm), resulting in specific energy inputs (2.5 22.5 kJ/kg). Numerous parameters whole and extracted juice were analyzed, including total phenolic index (TPI), reducing sugars, yields, changes color texture. Changes aroma profile investigated using...
Cells of a cell-wall deficient line (cw15-type) Chlamydomonas reinhardtii and the corresponding wild type were grown during repetitive light–dark cycles. In direct comparison, both lines showed approximately same relative biomass increase light phase but cw-line produced significantly more, smaller, daughter cells. Throughout period average cellular starch content, chlorophyll rate dark respiration, photosynthesis was lower. Despite this, several non-cell volume related parameters like...
In cultures of unicellular algae, features single cells, such as cellular volume and starch content, are thought to be the result carefully balanced growth division processes. Single-cell analyses synchronized photoautotrophic alga Chlamydomonas reinhardtii reveal, however, that content only weakly correlated. Likewise, other cell parameters, e.g., chlorophyll per cell, correlated with size. We derive size distributions at beginning each synchronization cycle considering growth, timing...
Accurate determination of the mixing time in bioreactors is essential for optimization productivity bioprocesses. The aim this work was to develop a simple optical method determine photobioreactor. image processing should be based on freeware tools, not require programming skills, and thus could used education within high schools early stages undergraduate programs.An has been established analyze images from recorded videos experiments. steps are: 1. Extraction sequence video file; 2....