A5006159573- Ion channel regulation and function
- Cardiac electrophysiology and arrhythmias
- Pain Mechanisms and Treatments
- Neuroscience and Neuropharmacology Research
- Electrochemical Analysis and Applications
- Cardiomyopathy and Myosin Studies
- Fatty Acid Research and Health
- Pain Management and Opioid Use
- Bipolar Disorder and Treatment
- Advanced biosensing and bioanalysis techniques
- Viral Infections and Immunology Research
- Ion Channels and Receptors
- Peroxisome Proliferator-Activated Receptors
- Cell Adhesion Molecules Research
- Mass Spectrometry Techniques and Applications
- Receptor Mechanisms and Signaling
- Neuroscience and Neural Engineering
- Adipose Tissue and Metabolism
- Atherosclerosis and Cardiovascular Diseases
- NF-κB Signaling Pathways
- Nicotinic Acetylcholine Receptors Study
- Pain Management and Treatment
Instituto de Investigaciones Biomédicas Sols-Morreale
2019-2024
Universidad Autónoma de Madrid
2019-2024
Instituto de Salud Carlos III
2021-2023
Centro de Investigación en Red en Enfermedades Cardiovasculares
2021-2023
Consejo Superior de Investigaciones Científicas
2023
Universidad Complutense de Madrid
2022
Resolution of inflammation is orchestrated by specialized proresolving lipid mediators (SPMs), and this would be impaired in some cardiovascular diseases. Among SPMs, resolvins (Rv) have beneficial effects pathologies, but little known about their effect on damage hypertension.Aorta, small mesenteric arteries, heart, peritoneal macrophages were taken from C57BL/6J mice, infused or not with angiotensin II (AngII; 1.44 mg/kg/day, 14 days) presence absence resolvin D2 (RvD2) (100 ng/mice, every...
Abstract Aim The voltage‐gated Kv7.1 channel, in association with the regulatory subunit KCNE1, contributes to I Ks current heart. However, both proteins travel plasma membrane using different routes. While KCNE1 follows a classical Golgi‐mediated anterograde pathway, is located endoplasmic reticulum‐plasma junctions (ER‐PMjs), where it associates before being delivered membrane. Methods To characterize channel routing these spots we used wide repertoire of methodologies, such as protein...
DREAM/KChIP3/calsenilin is a neuronal calcium sensor with multiple functions, including the regulation of A-type outward potassium currents (IA). This effect mediated by interaction between DREAM and KV4 channels it has been shown that small molecules bind to modify channel function. current (IA) responsible fast repolarization neuron action potentials frequency firing. Using surface plasmon resonance assays electrophysiological recordings KV4.3/DREAM channels, we have identified IQM-266 as...
Neuropathic pain is a form of chronic arising from damage the neural cells that sense, transmit or process sensory information. Given its growing prevalence and common refractoriness to conventional analgesics, development new drugs with relief effects constitutes prominent clinical need. In this respect, reduce activity neurons by modulating ion channels hold promise become effective analgesics. Here, we evaluated mechanical antinociceptive effect IQM-PC332, novel ligand multifunctional...
Abstract Background Novel developmental mutations associated with disease are a continuous challenge in medicine. Clinical consequences caused by these include neuron and cognitive alterations that can lead to epilepsy or autism spectrum disorders. Often, it is difficult identify the physiological defects appropriate treatments. Results We have isolated cultured primary cells from skin of patient combined syndrome. A mutation potassium channel protein Kv10.2 was identified. characterised...
The transient outward potassium current (Itof) is generated by the activation of KV4 channels assembled with KChIP2 and other accessory subunits (DPP6 KCNE2). To test hypothesis that these modify channel pharmacology, we analyzed electrophysiological effects (3-(2-(3-phenoxyphenyl)acetamido)-2-naphthoic acid) (IQM-266), a new ligand, on currents KV4.3/KChIP2, KV4.3/KChIP2/DPP6 KV4.3/KChIP2/KCNE2 channels. CHO cells were transiently transfected cDNAs codifying for different proteins...
ABSTRACT Background Patients with atrial fibrillation (AF) exhibit a reduction in the ultrarapid outward potassium current ( I Kur ) conducted by K V 1.5 channels. Ion channels are closely modulated regulatory subunits, forming macromolecular complexes known as channelosomes. One such family is leucine-rich glioma-inactivated protein (Lgi1-4), which has been shown to interact 1, modifying their trafficking and/or biophysical properties neurons. However, expression and impact of these...
The voltage-gated potassium channel Kv1.3 plays a crucial role in the immune system response. In leukocytes, is co-expressed with dominant negative regulatory subunit KCNE4, which associates to trigger intracellular retention and accelerate C-type inactivation of channel. Previous research has demonstrated that main association between these proteins occurs through both COOH-termini. However, data fail fully elucidate KCNE4-dependent modulation kinetics. present study, we analyzed...
Abstract Aims The resolution of inflammation is modulated by specialized pro-resolving lipid mediators (SPMs), which can be modified in some cardiovascular diseases. Among them, RvD1 and LXA 4 prevent atrial fibrillation (AF) remodeling the atria cardiac hypertrophy, respectively animal models. However, little known about their electrophysiological effects on voltage-gated (VG) ion channels. Methods results We used patch-clamp technique heterologous systems cardiomyocytes to assess acute...
Abstract Background Flecainide and other class-Ic antiarrhythmic drugs (AADs) are widely used in Andersen-Tawil syndrome type 1 (ATS1) patients. However, might be proarrhythmic some cases. We investigated the molecular mechanisms of class-I AADs proarrhythmia whether they increase risk death ATS1 patients with structurally normal hearts. Methods Results Of 53 reviewed from literature, 54% responded partially to flecainide, ventricular arrhythmia (VA) reduction only 23%. latter patients, VA...