A5030590587- Plant Reproductive Biology
- Plant tissue culture and regeneration
- Plant Genetic and Mutation Studies
- Plant Stress Responses and Tolerance
- Plant Molecular Biology Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Chromosomal and Genetic Variations
- Kidney Stones and Urolithiasis Treatments
- Seed Germination and Physiology
- Immune Cell Function and Interaction
- Lymphoma Diagnosis and Treatment
- Photosynthetic Processes and Mechanisms
- Plant-Microbe Interactions and Immunity
- Cancer Immunotherapy and Biomarkers
- Sunflower and Safflower Cultivation
- RNA modifications and cancer
- Cell Adhesion Molecules Research
- S100 Proteins and Annexins
- Acute Myocardial Infarction Research
- Protease and Inhibitor Mechanisms
- Lung Cancer Treatments and Mutations
- Immune cells in cancer
- Transgenic Plants and Applications
- Growth and nutrition in plants
- Cancer Treatment and Pharmacology
Hospital Universitario Puerta de Hierro Majadahonda
2014-2025
Universidad Autónoma de Madrid
2017
Hospital Universitario La Paz
2014
Leibniz Institute of Plant Genetics and Crop Plant Research
2005-2012
Centro de Investigaciones Biológicas Margarita Salas
2000-2011
Consejo Superior de Investigaciones Científicas
2004-2008
Universitat Politècnica de València
2008
Kazusa DNA Research Institute
2006
Estación Experimental de Aula Dei
2006
Unidades Centrales Científico-Técnicas
2005
Abstract Background In recent years, the application of nanotechnology in several fields bioscience and biomedicine has been studied. The use nanoparticles for targeted delivery substances given special attention is particular interest treatment plant diseases. this work both penetration movement iron-carbon cells have analyzed living plants Cucurbita pepo . Results were applied planta using two different methods, injection spraying, magnets used to retain particles specific areas plant....
† Background and Aims The great potential of using nanodevices as delivery systems to specific targets in living organisms was first explored for medical uses.In plants, the same principles can be applied a broad range uses, particular tackle infections.Nanoparticles tagged agrochemicals or other substances could reduce damage plant tissues amount chemicals released into environment.To explore benefits applying nanotechnology agriculture, stage is work out correct penetration transport...
Platinum-based chemotherapy remains the standard of care for most lung cancer cases. However chemoresistance is often developed during treatment, limiting clinical utility this drug. Recently, ability tumor cells to adapt their metabolism has been associated resistance therapies. In study, we first described metabolic reprogramming Non-Small Cell Lung Cancer (NSCLC) in response cisplatin treatment. Cisplatin-resistant versions A549, H1299, and H460 cell lines were generated by continuous...
Lung cancer is a major public health problem due to its high incidence and mortality rate. The altered metabolism in lung key for the diagnosis has implications on both, prognosis response treatments. Although Cancer-associated fibroblasts (CAFs) are one of components tumor microenvironment, little known about their role metabolism. We studied biopsies from cohort 12 stage IIIA adenocarcinoma patients saw positive correlation between grade fibrosis glycolysis phenotype (Low PGC-1α High...
Peritoneal adhesions (PAs) are fibrotic bands formed between bowel loops, solid organs, and the parietal peritoneum, which may appear following surgery, infection or endometriosis. They represent an important health problem with no effective treatment. Mesothelial cells (MCs) line peritoneal cavity undergo a mesothelial-to-mesenchymal transition (MMT) under pathological conditions, transforming into myofibroblasts, abundant in tissue. The aim of this study was to investigate if MCs MMT...
Severe coronavirus disease 2019 (COVID-19) is characterized by the intense formation of neutrophil extracellular traps (NETs), leading to occlusion microvessels, as shown in pulmonary samples. The occurrence ST-elevated myocardial infarction (STEMI) a serious cardiac manifestation COVID-19; intrinsic mechanism coronary thrombosis appears still be unknown.
Under specific stress treatments (cold, starvation), in vitro microspores can be induced to deviate from their gametophytic development and switch embryogenesis, forming haploid embryos homozygous breeding lines a short period of time. The inductive produces reactive oxygen species (ROS) nitric oxide (NO), signalling molecules mediating cellular responses, cell death, modifying the embryogenic microspore response therefore, efficiency process. This work analysed caspase 3-like activity, ROS...
Plant cells exhibit a high rate of mitochondrial DNA (mtDNA) recombination. This implies that before cytokinesis, the different compartments must fuse to allow for mtDNA intermixing. When and how conditions intermixing are established largely unknown. We have investigated cell cycle-dependent changes in architecture Arabidopsis (Arabidopsis thaliana) types using confocal microscopy, conventional, three-dimensional electron microscopy techniques. Whereas mitochondria from most plant organs...
Background and aims Inflammation plays a pivotal role in the pathophysiology of ST-elevation myocardial infarction (STEMI). This involves neutrophil activation local release pro-inflammatory mediators. The formation extracellular traps (NETs) coronary thrombosis has been linked to poor short-term prognosis following STEMI, but usefulness specific circulating NET components as prognostic markers is unclear. We aimed evaluate NET-specific marker nucleosomal citrullinated histone H3 (H3Cit-DNA)...
Two types of Tn1732-induced mutants defective in extracellular amylase activity were isolated from the moderate halophile Halomonas meridiana DSM 5425. Type I displayed periplasm, and unable to use any carbon sources tested, including starch its hydrolysis product maltose. The type II mutant was affected gene responsible for synthesis α-amylase. This (amyH) by functional complementation sequenced. deduced protein (AmyH) showed a high degree homology proposed family α-amylases consisting...
Abstract Ascorbic acid (vitamin C) is a major antioxidant and redox buffer, but also involved in other critical processes of plants. Recently, the hypothesis has been proposed that legume nodules are unable to synthesize ascorbate have import it from shoot or root, thus providing means by which plant regulates nodule senescence. The last step biosynthesis plants catalyzed l-galactono-1,4-lactone dehydrogenase (GalLDH). mRNAs encoding GalLDH three enzymes clearly detectable nodules....
Following the production of transgenic plants, selectable marker gene(s) used in process are redundant, and their retention may be undesirable. They can removed by exploiting segregation among progeny co-transformants carrying both gene effector transgene. Here we show that doubled haploid technology widely conventional barley breeding programmes represents a useful means fixing transgene, while simultaneously removing unwanted gene. Primary involving hpt::gfp (the marker) gus (a model...
The alterations of organization leaf tissues and cell ultrastructure as a consequence salt stress (75 150 mM NaCl) were studied in two tomato (Lycopersicum esculentum Mill.) cultivars showing different salinity tolerance. brought changes shape, volume intercellular spaces chloroplast number, shape size. These characteristics specific each cultivar. ultrastructural also the most important ones number size starch granules chloroplasts, electron-dense corpuscules cytoplasm, structure...
Abstract Immunotherapies, such as checkpoint blockade of programmed cell death protein-1 (PD-1), have resulted in unprecedented improvements survival for patients with lung cancer. Nonetheless, not all benefit equally and many issues remain unresolved, including the mechanisms action possible effector function immune cells from non-lymphoid lineages. The purpose this study was to investigate whether anti-PD-1 immunotherapy acts on malignant tumor through beyond those related T lymphocyte...
Abstract Aim The voltage‐gated Kv7.1 channel, in association with the regulatory subunit KCNE1, contributes to I Ks current heart. However, both proteins travel plasma membrane using different routes. While KCNE1 follows a classical Golgi‐mediated anterograde pathway, is located endoplasmic reticulum‐plasma junctions (ER‐PMjs), where it associates before being delivered membrane. Methods To characterize channel routing these spots we used wide repertoire of methodologies, such as protein...