A5009874765- Nanoplatforms for cancer theranostics
- Photodynamic Therapy Research Studies
- Luminescence and Fluorescent Materials
- Cell Image Analysis Techniques
- Advanced Fluorescence Microscopy Techniques
- Photoreceptor and optogenetics research
- Developmental Biology and Gene Regulation
- Photochromic and Fluorescence Chemistry
- Click Chemistry and Applications
- Ion channel regulation and function
- Gut microbiota and health
- Dental Implant Techniques and Outcomes
- Lipid Membrane Structure and Behavior
- Supramolecular Self-Assembly in Materials
- Neuroscience and Neural Engineering
- Bone Tissue Engineering Materials
- Hippo pathway signaling and YAP/TAZ
- Neurobiology and Insect Physiology Research
- Cardiac electrophysiology and arrhythmias
- Silk-based biomaterials and applications
- Barrier Structure and Function Studies
- Nanoparticle-Based Drug Delivery
- Porphyrin and Phthalocyanine Chemistry
- Cancer, Hypoxia, and Metabolism
- Single-cell and spatial transcriptomics
Universitat de Barcelona
2016-2025
Institut de Biomedicina de la Universitat de Barcelona
2010
University of Sussex
2010
The gastrointestinal epithelial layer forms a physical and biochemical barrier that maintains the segregation between host intestinal microbiota. integrity of this is critical in maintaining homeostasis body its dysfunction linked to variety illnesses, especially inflammatory bowel disease. Gut microbes, particularly probiotic bacteria, modulate by reducing gut permeability reinforcing tight junctions. Probiotic Escherichia coli Nissle 1917 (EcN) good colonizer human with proven therapeutic...
Among the palette of previously described fluorescent organic molecules, coumarins are ideal candidates for developing cellular and molecular imaging tools due to their high cell permeability minimal perturbation living systems. However, blue-to-cyan fluorescence emission is usually difficult in vivo applications inherent toxicity poor tissue penetration short visible light wavelengths. Here, we introduce a new family coumarin-based fluorophores, nicknamed COUPY, with promising photophysical...
Photodynamic therapy (PDT) represents a promising approach for cancer treatment. However, the oxygen dependency of PDT to generate reactive species (ROS) hampers its therapeutic efficacy, especially against hypoxic solid tumors. In addition, some photosensitizers (PSs) have dark toxicity and are only activatable with short wavelengths such as blue or UV-light, which suffer from poor tissue penetration. Herein, we developed novel hypoxia-active PS operability in near-infrared (NIR) region...
Despite the potential of photodynamic therapy (PDT) in cancer treatment, development efficient and photostable photosensitizing molecules that operate at long wavelengths light has become a major hurdle. Here, we report for first time an Ir(III)-phthalocyanine conjugate (
Symbiotic interactions between ascidians (sea-squirts) and microbes are poorly understood. Here we characterized the cyanobacteria in tissues of 8 distinct didemnid taxa from shallow-water marine habitats Bahamas Islands by sequencing a fragment cyanobacterial 16S rRNA gene entire 16S–23S internal transcribed spacer region (ITS) examining symbiont morphology with transmission electron (TEM) confocal microscopy (CM). As described previously for other species, Trididemnum spp. mostly contained...
Photodynamic therapy (PDT) for cancer treatment has drawn increased attention over the last decades. Herein, we introduce a novel family of low-molecular-weight coumarins as potential PDT anticancer tools. Through systematic study with library 15 compounds, have established detailed structure–activity relationship rationale, which allowed selection three lead compounds exhibiting effective in vitro activities upon visible-light irradiation both normoxia and hypoxia (phototherapeutic indexes...
Photodynamic therapy holds great promise as a non-invasive anticancer tool against drug-resistant cancers. However, highly effective, non-toxic, and reliable photosensitizers with operability under hypoxic conditions remain to be developed. Herein, we took the advantageous properties of COUPY fluorophores cyclometalated Ir(III) complexes develop novel PDT agents based on Ir(III)-COUPY conjugates aim exploring structure–activity relationships. The structural modifications carried out within...
A modern day light microscope has evolved from a tool devoted to making primarily empirical observations what is now sophisticated , quantitative device that an integral part of both physical and life science research. Nowadays, microscopes are found in nearly every experimental laboratory. However, despite their prevalent use capturing quantifying scientific phenomena, neither thorough understanding the principles underlying imaging techniques nor appropriate knowledge how calibrate,...
Integration of photosensitizers (PSs) within nanoscale delivery systems offers great potential for overcoming some the "Achiles' heels" photodynamic therapy (PDT). Herein, we have encapsulated a mitochondria-targeted coumarin PS into amphoteric polyurethane-polyurea hybrid nanocapsules (NCs) with aim developing novel nanoPDT agents. The synthesis coumarin-loaded NCs involved nanoemulsification suitable prepolymer in presence without needing external surfactants, and resulting small...
Light-activated treatments, such as photodynamic therapy (PDT), provide temporal and spatial control over a specific cytotoxic response by exploiting toxicity differences between irradiated dark conditions. In this work, novel strategy for developing near infrared (NIR)-activatable Ru(II) polypyridyl-based photosensitizers (PSs) was successfully developed through the incorporation of symmetric heptamethine cyanine dyes in metal complex via phenanthrimidazole ligand. Owing to their strong...
Inflammatory bowel disease (IBD) is a chronic inflammatory condition involving dysregulated immune responses and imbalances in the gut microbiota genetically susceptible individuals. Current therapies for IBD often have significant side-effects limited success, prompting search novel therapeutic strategies. Microbiome-based approaches aim to restore balance towards anti-inflammatory mucosa-healing profiles. Extracellular vesicles (EVs) from beneficial microbes are emerging as potential...
Hypoxia, a hallmark of many solid tumors, is linked to increased cancer aggressiveness, metastasis, and resistance conventional therapies, leading poor patient outcomes. This challenges the efficiency photodynamic therapy (PDT), which relies on generation cytotoxic reactive oxygen species (ROS) through irradiation photosensitizer (PS), process partially dependent levels. In this work, we introduce novel family potent PSs based ruthenium(II) polypyridyl complexes with 2,2′-bipyridyl ligands...
Despite the potential of photodynamic therapy (PDT), this oxygen-dependent oncological treatment is greatly restricted in clinic by well-known hypoxic feature solid tumors. Here we provide new insights...
Abstract Background Regeneration is the ability of an organism to rebuild a body part that has been damaged or amputated, and can be studied at molecular level using model organisms. Drosophila imaginal discs, which are larval primordia adult cuticular structures, capable undergoing regenerative growth after transplantation in vivo culture into abdomen. Results Using expression profile analyses, we behaviour wing discs 0, 24 72 hours fragmentation implantation females. Based on level,...
Abstract Background Resonance frequency analysis ( RFA ) is applied clinically for the assessment of implant stability, and relevance this application widely accepted. However, relationship between resonance RF other parameters such as histomorphometrical bone‐to‐implant contact BIC parameter, has become controversial in last decade. Objective To analyse clarify measurements. Material methods A total 36 dental implants (9 mm length, Ø 4.0 mm; Biohorizons ® Implant Systems Inc., B irmingham,...
Trefoil factor 3 (TFF3) plays a key role in the maintenance and repair of intestinal mucosa. TFF3 expression is upregulated by microbiota through TLR2. At posttranscriptional level, downregulated miR-7-5p. Reduced levels have been detected damaged tissue IBD patients. Here, we investigate regulation extracellular vesicles (EVs) LS174T goblet cells using RT-qPCR inhibitors TLR2 or PI3K pathways. To evaluate subsequent impact on epithelial barrier function, conditioned media from control...
Following a period of neglect, there has been resurgence interest in <i>Drosophila</i> imaginal discs as model with which to analyze the relationships between growth and pattern formation during regeneration. To broaden our understanding this process, we used cell lineage techniques trace origin blastema cells early late boundaries regenerating 3/4 wing disc fragments, examined distribution S-phase, mitotic dead cells, undertook clonal analysis determine topology proliferation its...
Replacement of electron-donating N,N-dialkyl groups with three- or four-membered cyclic amines (e.g., aziridine and azetidine, respectively) has been described as a promising approach to improve some the drawbacks conventional fluorophores, including low fluorescent quantum yields (ΦF) in polar solvents. In this work, we have explored influence azetidinyl substitution on nonconventional coumarin-based COUPY dyes. Two azetidine-containing scaffolds were first synthesized four linear synthetic...
Mitochondrial dysfunction has been associated with several human pathological conditions, including cancer, aging, and neurodegenerative diseases. Thus, the availability of selective fluorescent probes for mitochondria could play an important role in future monitoring cellular functions disease progression. In this work, we have studied how photophysical properties subcellular accumulation nonconventional coumarin-based COUPY fluorophores can be fine-tuned through replacement para-pyridinium...
Fluorophores based on organic molecules hold great potential for ligand-targeted imaging applications, particularly those operating in the optical window biological tissues. In this work, we have developed three straightforward solid-phase approaches amide-bond formation or a Cu(I)-catalyzed azide–alkyne click (CuAAC) reaction labeling an octreotide peptide with far-red emitting coumarin-based COUPY dyes. First, conjugatable versions of fluorophores incorporating required functional groups...
Polyurethane–polyurea hybrid nanocapsules can be used as efficient delivery systems of anticancer Ir( iii ) metallodrugs.
Abstract Aim The voltage‐gated Kv7.1 channel, in association with the regulatory subunit KCNE1, contributes to I Ks current heart. However, both proteins travel plasma membrane using different routes. While KCNE1 follows a classical Golgi‐mediated anterograde pathway, is located endoplasmic reticulum‐plasma junctions (ER‐PMjs), where it associates before being delivered membrane. Methods To characterize channel routing these spots we used wide repertoire of methodologies, such as protein...
Abstract Single‐bath potentiostatic‐pulsed electrodeposition enables the synthesis of bicomponent (i.e., gold and nickel–nickel oxide) multi‐segmented magnetic nanowires that, with extraordinarily low cytotoxicity, are ideal three‐functional medical nanoplatforms because they can transport two types functional molecules be magnetically actuated for both controlled targeting inducing cancer cell death. Alternated segments Au Ni–Ni oxide selected to confer a character nanowires, prevent their...